The amplitude of coagulation curves from thrombin time tests allows dysfibrinogenemia caused by the common mutation FGG‐Arg301 to be distinguished from hypofibrinogenemia

Jacquemin, Marc; Vanlinthout, Ingrid; Horenbeeck, Isa Van; Debasse, Mirjam; Toelen, Jaan; Schoeters, Joke; Lavend’homme, Renaud; Freson, Kathleen; Peerlinck, Kathelijne

doi:10.1111/ijlh.12625

Cited by 11 publications

(14 citation statements)

References 22 publications

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“…We also compared Fibrinography to the widely used turbidity measurement performed at a single wavelength (e.g., 7 and references herein). Our results demonstrate that measuring the optical density at a single wavelength mainly determines the fibrinogen concentration in the sample, as expected from the similarity of this assay with the prothrombin time-derived fibrinogen determination, 27–29 rather than the structure of the clot. Therefore, maximum turbidity in plasma clots is not a determinant of fibrin's structure, but is rather a measure of the fibrinogen concentration, even in the case of patients’ plasma as shown recently by Jacquemin et al 29 Besides, when comparing data from patients (Fig.…”

Section: Discussionsupporting

confidence: 73%

Fibrinography: A Multiwavelength Light‐Scattering Assay of Fibrin Structure

et al. 2019

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We have previously developed a fibrin structural assay dedicated to purified fibrinogen-thrombin system. Here, we extend the pertinence of this test, called Fibrinography, to tissue factor-triggered plasma coagulation. We show that Fibrinography determines quantitatively the structure of fibrin fibers in plasma with an excellent reproducibility. We compare this assay with the commonly used single wavelength turbidity method, showing that the latter is not a proper structural assay, but determines essentially the fibrinogen content in plasma. In addition, we also show, in model plasmas, that Fibrinography is able to discriminate normal and hypocoagulant plasmas, and even between hypercoagulant plasmas. Therefore, Fibrinography, by measuring the final step of the coagulation cascade, may be used to evaluate patients’ plasma in hypo- or hypercoagulant diseases.

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Section: Discussionsupporting

confidence: 73%

Fibrinography: A Multiwavelength Light‐Scattering Assay of Fibrin Structure

et al. 2019

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“…A ratio of functional on antigenic fibrinogen level higher than 0.7 is traditionally used to distinguish between hypofibrinogenemia and dysfibrinogenemia [ 8 ], although the sensitivity and specificity of this cut-off has never been established [ 9 ]. The amplitude of coagulation curves from thrombin time tests should allow for the easy determination of the type of fibrinogen deficiency when the antigenic fibrinogen assessment is not available [ 10 ]. Due to the limited sensitivity of coagulation assays in the case of very low fibrinogen levels (e.g., <0.5 g/L), it can be challenging to distinguish afibrinogenemia from severe hypofibrinogenemia [ 11 ].…”

Section: Diagnosis Of Quantitative Fibrinogen Disordersmentioning

confidence: 99%

Clinical Consequences and Molecular Bases of Low Fibrinogen Levels

Neerman-Arbez

Casini

2018

IJMS

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Abstract:The study of inherited fibrinogen disorders, characterized by extensive allelic heterogeneity, allows the association of defined mutations with specific defects providing significant insight into the location of functionally important sites in fibrinogen and fibrin. Since the identification of the first causative mutation for congenital afibrinogenemia, studies have elucidated the underlying molecular pathophysiology of numerous causative mutations leading to fibrinogen deficiency, developed cell-based and animal models to study human fibrinogen disorders, and further explored the clinical consequences of absent, low, or dysfunctional fibrinogen. Since qualitative disorders are addressed by another review in this special issue, this review will focus on quantitative disorders and will discuss their diagnosis, clinical features, molecular bases, and introduce new models to study the phenotypic consequences of fibrinogen deficiency.

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“…On the other hand, FGG, a gene encoding γ component of fibrinogen, has vasonstriction and chemotactic activities that regulates cell adhesion and spreading [41]. Upregulation of the gene in SCLC can be correlated with high metastatic capability of the subtype.…”

Section: Discussionmentioning

confidence: 99%

“…FGG, as discussed before is a gene encoding γ component of fibrinogen, which is involved in the process of blood clotting. Any perturbations in these genes are associated with the homeostatic imbalance between coagulation and anticoagulation [41]. Thus, differentially expressed FGG might contribute to the processes of pathologic thrombosis and angiogenesis associated with cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes in small and non-small cell lung cancer based on meta-analysis of mRNA

Shriwash

Singh

Arora

et al. 2019

Heliyon

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Lung cancer has the lowest survival rate spread globally resulting in a large number of deaths. This is attributed to insufficient measures such as lack of early detection and chemoresistance in the patients. It can be subdivided into two histological groups: Non-Small-Cell Lung Cancer (NSCLC), which is most prevalent (85% of all lung cancers) but less destructive; and Small-Cell Lung Cancer (SCLC), which is intermittently metastatic and less prevalent (15% of all lung cancers). The present study deals with the analysis of gene expression of two subtypes to identify the Differentially Expressed Genes (DEGs). For this study, we selected two datasets from the Omnibus database, which included 50 non-small cell lung cancer samples, 31 small cell lung cancer samples, and 48 samples from normal lung tissue. After DEGs identification using the meta-analysis approach, they were then subjected to further analysis following p-value adjustment via the Benjamini-Hochberg method. We identified 440 overexpressed and 489 underexpressed genes in NSCLC, and 489 overexpressed and 525 underexpressed genes in SCLC, compared with normal lung tissues. Furthermore, we identified 3 overlapping genes between upregulated DEGs in NSCLC and downregulated DEGs in SCLC; and 8 overlapping genes between upregulated DEGs in SCLC and downregulated DEGs in NSCLC. Accordingly, a Protein-Protein Interaction (PPI) network of the overlapping genes was generated, which contained a total of 261 genes, of which the top five were TRIM29, ANK3, CSTA, FGG, and AGR2. These five candidate genes reported herein may prove to be potential therapeutic targets.

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The amplitude of coagulation curves from thrombin time tests allows dysfibrinogenemia caused by the common mutation FGG‐Arg301 to be distinguished from hypofibrinogenemia

Abstract: Examination of the amplitude of coagulation curves generated during TT tests may provide additional information to enable the differential diagnoses of diseases following a low fibrinogen measurement by the Clauss method.

Cited by 11 publications

References 22 publications

Fibrinography: A Multiwavelength Light‐Scattering Assay of Fibrin Structure

Fibrinography: A Multiwavelength Light‐Scattering Assay of Fibrin Structure

Clinical Consequences and Molecular Bases of Low Fibrinogen Levels

Identification of differentially expressed genes in small and non-small cell lung cancer based on meta-analysis of mRNA

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