The analysis of actinomycete wall amino acids by gas chromatography

Abstract

ErratumThis article has been published earlier in FEMS Microbiology Letters, 15 (1982) 75-78. Due to a technical error the wrong Figures 1 and 2 were included. Therefore, the article is republished--with apologies to the authors--with the correct figures. The Publishers

Identification of bacterial isolates from biofilters as Paracoccus alkenifer sp. nov. and Paracoccus solventivorans with emended description of Paracoccus solventivorans

“…Cells were washed and lyophilized. Cell wall amino acids were purified and transformed to their heptafluorobutyryl isobutyl esters as described by O'Donnell et al (20). The amino acid esters were separated and identified by GC-MS with a HewlettPackard model 5890 series I1 gas chromatograph equipped with a 5 % phenylmethyl silicone capillary column (025 mm by 30 rn) and a model 5972 mass selective detector.…”

Arthrobacter bussei sp. nov., a pink-coloured organism isolated from cheese made of cow’s milk

“…Isolates were grown at 30 °C for 24 h in TSB, harvested by centrifugation, washed and lyophilised. Cell-wall amino acids were purified and derivatized to their heptafluorobutyryl isobutyl esters as described by O'Donnell et al [52]. Samples were analysed on an Agilent 7890A/5975C GC-MSD system equipped with an HP5-MS column.…”

Hongia gen. nov., a new genus of the order Actinomycetales.

“…All of the -DAP-containing actinomycete genera, with the exception of K. aurantiaca, have phospholipid type I or II patterns according to Lechevalier et al (1981) in that phosphatidylcholine or gucosaminecontaining phospholipids were absent from their phospholipid compositions (Prauser et al, 1997 ;Schumann et al, 1997). The diagnostic phospholipid profiles (of the phospholipid type III pattern ; Table 2) and the cellular fatty acid profiles of the isolate (Table 1) are clearly distinguishable from those of actinomycete genera with -DAP in their peptidoglycan (Collins et al, 1989 ;Miller et al, 1991 ;Nakamura et al, 1995 ;O'Donnell et al, 1982b ;Prauser et al, 1997 ;Rainey et al, 1993 ;Schumann et al, 1997 ;Williams et al, 1989). Although K. aurantiaca is similar to the isolate in terms of menaquinone types and amino acids in the peptide subunit of the peptidoglycan, as well as in terms of phospholipid composition, it differs from the isolate in having meso- Hongia gen. nov.…”

“…Purified cell wall peptidoglycan was prepared according to the method of Yamada & Komagata (1972) and its amino acid composition was analysed by twodimensional, ascending TLC on cellulose plates by using the solvent systems described previously (Schleifer & Kandler, 1972). The molar ratio of amino acids was determined by GC of N-heptafluorobutyryl amino acid isobutyl esters (O'Donnell et al, 1982b). The isomers of diaminopimelic acid were determined according to the method of Staneck & Roberts (1974).…”