The analysis of blood flow indicators in the microvascular bed of the human skin and their relationship with central hemodynamic parameters

Introduction. For the treatment of patients with hypoxemic form of respiratory failure, thermal oxygen-helium mixture (OHM) therapy is used.The aim of the work is to study the effect of OHM on the state of the microcirculatory bed in viral pneumonia caused by COVID-19 in patients with aggravated and unaggravated anamnesis.Materials and methods. There were 8 patients with an uncomplicated anamnesis, 11 with AH1–2 degree, 11 with AH1–2 degree and type II diabetes. The parameters of blood flow in the MVB were recorded with an ultrasound dopplerograph. OHM therapy (O2 – 30 %, He – 70 %, t=+95 ºС): 5 min breathing, 5 min rest – 3 cycles.Results. Before the OHM therapy, the level of «basic perfusion» was increased in group 3: significantly high Vs (p=0.02) and Vd (p=0.006), low RI (p=0.015) relative to group 1, this indicates a reduced vascular tone and venous congestion. After the OHM session in patients of group 1, the speed of Vs and Vd increased, the indices RI and PI decreased. In group 2 – Vs, Vd and Vam increased, RI decreased. The effect is long lasting. In group 3, the effect was noted on day 5, short-term. Conclusions. OHM therapy has a positive effect.

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The relationship of hemorheological blood values and blood velocity of microcirculatory bloodstream in rats` skin vessels

Petrishchev,

Skedina,

Grishacheva

et al. 2024

Rossijskij fiziologičeskij žurnal im. I.M. Sečenova

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Non-invasive study of blood rheology is relevant, but quite complex issue. When systemic blood viscosity and hematocrit levels deviate, blood flow indicators in different parts of microvasculature change. Purpose of the study – research of blood flow characteristics in skin microcirculatory bloodstream of rats obtained by high-frequency ultrasound Dopplerography (HFUD) with given changes in rheological blood indicators. The studies were carried out on pubescent male Wistar rats. 3 experimental groups were formed. Group 1 (n = 21) “Hemodilution” – viscosity 1.99 ± 0.02 mPa*s, hematocrit 31.48 ± 0.31%. Group 2 (n = 32) “Reference values” – animals with unchanged blood levels – viscosity 2.84 ± 0.03 mPa*s, hematocrit 41.60 ± 0.3%. Group 3 (n = 32) “Erythrocytosis” – viscosity 3.95 ± 0.04 mPa*s, hematocrit 54.56 ± 0.23%. Dynamic blood viscosity in vitro studies were carried out on oscillatory viscometer. In order to evaluate hematocrit level heparinized whole blood was centrifuged in glass capillaries using; hematocrit values were assessed taking into account sedimentation of formed elements column using a hematocrit reader card. Blood flow in skin microcirculatory bloodstream of rats` left thigh area was estimated by HFUD method using Minimax-Doppler-K hardware and software system, with ultrasound transducer (frequency 20 MHz). Statistical analysis showed the models are correct. Blood indicators of the animals in three experimental groups differed statistically and significantly in terms of blood viscosity and hematocrit. Discriminant analysis was used to determine the relations between rheological blood parameters and characteristics of blood velocity in microcirculatory bloodstream which made it possible to identify the most significant characteristics of blood flow that tend to change depending on altered blood composition. These include: mean systolic velocity Vas (p 0.01), mean velocity Vam (p 0.001), mean volume velocity Qam (p 0.001), vascular resistance index RI (p 0.01) and the percentage of blood cells moving in low-speed H' (p = 0.03). The reliability of selected characteristics was checked with one-way analysis of variance; and their significance in determining membership in “Hemodilution”, “Reference values” or “Erythrocytosis” groups according to HFUD data was confirmed. Based on this analysis classification functions were generated for non-invasive dynamic blood viscosity determination according to ultrasound Dopplerography data.

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The analysis of blood flow indicators in the microvascular bed of the human skin and their relationship with central hemodynamic parameters

Cited by 3 publications

References 13 publications

The Relationship of Hemorheological Blood Values and Blood Velocity of Microcirculatory Bloodstream in Rats’ Skin Vessels

The Relationship of Hemorheological Blood Values and Blood Velocity of Microcirculatory Bloodstream in Rats’ Skin Vessels

Study of the response of the microvascular bed to oxygen-helium therapy using high-frequency Doppler ultrasound in patients with COVID-19

The relationship of hemorheological blood values and blood velocity of microcirculatory bloodstream in rats` skin vessels

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