The analysis of combustion products

Egerton, Alfred Charles; Everett, A. J.; Minkoff, G. J.; Rudrakanchana, S.; Salooja, K.C.

doi:10.1016/s0003-2670(00)89683-x

Cited by 103 publications

(18 citation statements)

References 4 publications

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“…Cell protein -was assayed by the method of Lowry et al (1951), with crystalline bovine serum albumin as the standard. H202 was determined as described by Egerton et al (1954). fl-Carotene was measured by centrifuging culture medium to which fl-carotene had been added, at 2500rev./min for lOmin in an MSE bench centrifuge to remove any undissolved fl-carotene.…”

Section: Methodsmentioning

confidence: 99%

Induction of benzo[a]pyrene Mono-oxygenase in liver cell culture by the photochemical generation of active oxygen species. Evidence for the involvement of singlet oxygen and the formation of a stable inducing intermediate

Paine

1976

Biochemical Journal

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1. The photochemical generation of excited states of oxygen in liver cell culture by the mild ilumination of culture medium containing riboflavin, results in stimulation of benzo[a]pyrene 3-mono-oxygenase, a cytochrome P-450-linked mono-oxygenase. 2. The same large increase in mono-oxygenase activity was found when medium containing riboflavin was illuminated in the absence of cells and then stored in the dark for 24h before contact with the cells. From this it may be inferred that stimulation is due to the formation of a stable inducer in the culture medium. Further experiments indicate that the stable inducer is due to the photo-oxidation of an amino acid. 3. Evidence that singlet oxygen is responsible for initiating the stimulation of the mono-oxygenase is based on the use of molecules that scavenge particular active oxygen species. Of all the scavengers tested, only those that scavenge single oxygen inhibited the stimulation. 4. A hypothesis is developed to relate the stimulation of the mono-oxygenase by singlet oxygen in cultured cells to the regulation of the cytochrome P-450 enzyme system in vivo. It is suggested that single oxygen generation within cells may be a common factor linking the many structurally diverse inducers of the enzyme system.

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Section: Methodsmentioning

confidence: 99%

Induction of benzo[a]pyrene Mono-oxygenase in liver cell culture by the photochemical generation of active oxygen species. Evidence for the involvement of singlet oxygen and the formation of a stable inducing intermediate

Paine

1976

Biochemical Journal

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“…The titanous sulphate test [7] gave no indication of hydrogen peroxide in the reaction products. Hydrogen was not detected in the gaseous product.…”

Section: Resultsmentioning

confidence: 89%

Slow Combustion of Methane at Elevated Pressures

Tripathy

1975

Israel Journal of Chemistry

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Methane was partially oxidized with oxygen in a batch reactor at pressures of 1700 and 3400 atm and temperatures of 270–480°C. Residence times ranged from 0 to 60 min. Water, methanol, formaldehyde, acetone, carbon monoxide and carbon dioxide were produced in every reaction. In addition, ethanol was formed at high temperatures under nonisothermal conditions and formic acid was detected in reactions at 3400 atm. Products analyses were made with a two‐column gas chromatograph.

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“…To reduce hydroperoxides in the samples tested, two reducing columns were developed. The first was based on the use of SnCIR as described by Egerton et al (23). The sample to be reduced was passed through an l l-mm id x 26-cm column containing 4 g Celite impregnated with 1 g SnCI2 in 2 mL 0.1 N NaOH.…”

Section: Methodsmentioning

confidence: 99%

Quantification of carbonyl compounds in oxidized fats as trichlorophenylhydrazones

White

Hammond

1983

J Americ Oil Chem Soc

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A rapid determination of the carbonyl compounds in oxidized fats and oils was devised based on formation of trichlorophenylhydrazones (TCPH). Fat in cyclohexane/ether (99:1) was passed through a Florisil column to remove interfering hydrocarbons, and the fat and carbonyl compounds were eluted with ether. The eluate was reacted with trichlorophenylhydrazine with Florisil as a catalyst, and the TCPH were isolated from the fat by chromatography on a Florisil column. The TCPH were separated and quantified by gas chromatography on a 10‐m SE 30 capillary column. The method was applied to soybean oils stored under various conditions. The method also was applied to linoleic acid hydroperoxide to determine the extent to which decomposition of peroxides gave rise to carbonyl artifacts during the procedure. Although artifacts were minimal, they were reduced even further by passing the fat through a column of Celite impregnated with stannous chloride to reduce hydroperoxides before the carbonyl analysis.

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The analysis of combustion products

Cited by 103 publications

References 4 publications

Induction of benzo[a]pyrene Mono-oxygenase in liver cell culture by the photochemical generation of active oxygen species. Evidence for the involvement of singlet oxygen and the formation of a stable inducing intermediate

Induction of benzo[a]pyrene Mono-oxygenase in liver cell culture by the photochemical generation of active oxygen species. Evidence for the involvement of singlet oxygen and the formation of a stable inducing intermediate

Slow Combustion of Methane at Elevated Pressures

Quantification of carbonyl compounds in oxidized fats as trichlorophenylhydrazones

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