The Analysis of Heavy Metal Concentrations in Eggs of Collared Flycatchers, Ficedula Albicollis (Passeriformes, Muscicapidae), and Tits, Parus Major, Parus Caeruleus (Passeriformes, Paridae), in Different Areas of North-Eastern Ukraine

Чаплыгина, А. Б.; Yuzyk, D. I.

doi:10.1515/vzoo-2016-0030

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…Adults of Parus major Linnaeus, 1758 and, more oft en, the younger birds of this species fl y hundreds of kilometers away for wintering. Th ose birds migrate mainly from west to east than from north to south and back (Poluda, 2011). In such areas, ixodid ticks can create spontaneous foci (Nebogatkin, 1996), which either become permanent or decline.…”

Section: Resultsmentioning

confidence: 99%

Distribution of Ixodes ricinus (Arachnida, Ixodidae) in Ukraine in the Context of Tick Hazard, and Factors Favoring its Persistence in Conditions of Fast-Going Environmental Change

Akimov

Nebogatkin

2022

Zoodiversity

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Habitat conditions and factors were studied contributing to the spread of I. ricinus over vast territories. There are excellent conditions for the existence of hard ticks in the settlement agglomerations throughout Ukraine: suitable biotopes with expedient litter and a large number of hosts for all stages of development of ticks. The castor bean tick lives in all of Ukraine, adapting its size of idiosome and diapause to changing environmental conditions, and using the ability to parasitize on animals well adapted to urban conditions. I. ricinus prefers urban landscapes in areas with unfavorable environmental conditions. The hiatus in the range of the castor bean tick between the southern mainland of Ukraine and the steppe regions of Crimea may be restored due to global climatic changes.

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Section: Resultsmentioning

confidence: 99%

Distribution of Ixodes ricinus (Arachnida, Ixodidae) in Ukraine in the Context of Tick Hazard, and Factors Favoring its Persistence in Conditions of Fast-Going Environmental Change

Akimov

Nebogatkin

2022

Zoodiversity

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“…In general, it should be noted that if it is necessary to study exclusively the sex of the bird by PCR, the parameters that influence the choice of tissue for use as a sample are the degree of discomfort caused in the animal during sampling, and the degree of success of the analysis after using a certain type. In our case, DNA was 100.0% isolated from blood samples and the follicle of feathers, but nevertheless we prefer the selection of biomaterial in the form of venous blood, since in addition to molecular genetic studies, for example, a clinical blood test can also be carried out (Drahulian et al, 2018a) or the blood can be examined for the presence of heavy metals (Chaplygina & Yuzyk, 2016).…”

Section: Discussionmentioning

confidence: 98%

Sex identification of different species of wild birds using a single universal protocol to the bird sexing method based on gene polymorphism

Rudaуа

Katerynych

Drahulian³

et al. 2020

Regul. Mech. Biosyst.

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This article presents an elaboration of the protocol for the method of sexing wild birds based on the polymorphism of the CHD gene using P2/P8 primer for Common Pheasant – Phasianus colchicus (Linnaeus, 1758; Galliformes, Phasianidae); Silver Lofur or Silver Pheasant – Lophura nycthemera (Linnaeus, 1758; Galliformes, Phasianidae), Budgerigar – Melopsittacus undulatus (Shaw, 1805; Psittaciformes, Psittacidae), Herring Gull – Larus argentatus (Pontoppidan, 1763; Charadriiformes, Laridae), and White Stork – Ciconia ciconia (Linnaeus, 1758; Ciconiiformes, Ciconiidae). Blood samples were taken from Common Pheasant, Silver Pheasant and White Stork using the “drop of blood on paper” method. For the Budgerigar and the Herring Gull, DNA was isolated from the feather follicle. To isolate DNA, a commercial NeoPrep 100 DNA reagent kit (Neogen, Ukraine) was used. Primers P2/P8 were used for PCR; PCR was performed using GenPac PCR Core reagents (Neogen, Ukraine). We selected the optimal amount of Tag polymerase, the amount of DNA and primers and, according to the amount of reagents, set acceptable amplification modes and electrophoresis agarose gel percentage. Prior to PCR, additional DNA gel electrophoresis purification is proposed, which increases the percentage of positive sex determination results. It was found that the ideal mixture for the 5 bird species was an amplification mixture (total volume 20 µL, containing 1 U Tag polymerase, 100 ng DNA and 0.6 µM of each primer). The amplified CHD-Z fragment of Common and Silver pheasants is of ~340 n. p., CHD-W ~360 n. p. Herring Gull and Budgerigar have ~350 n. p. of CHD-Z length, and ~400 n. p. of CHD-W length, White Stork has its CHD-Z of ~ 370 n. p. long. It is advisable to investigate the genome of the experimental bird species using horizontal electrophoresis in agar’s gel with the concentration of 5%, which makes it possible to clearly visualize the female genotype. The universal protocol of the method of sex determination based on polymorphism of the CHD gene for the 5 studied bird species is described. These results of the study led to the conclusion that for the simultaneous sexing of several species of birds, it is advisable to develop a unified protocol for determining the status of the CHD gene, with the aim of clarifying the gender, as well as new approaches in ornithology and ecology aimed at determining interspecific differences associated with gene polymorphism. Identification of differences in fragment sizes may be useful for identifying the species in cases when birds form mixed pairings for taxonomic and phylogenetic comparisons.

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“…This result showed an increase in Cd with the three adsorbents which proved that some level of concentration of Cd is present in all the adsorbents which is in accordance to the literature especially in ES. Birds eliminate heavy metals from their body through their discharges and eggs that they lay [49,50].…”

Section: Heavy Metalsmentioning

confidence: 99%

Treatment of Poultry Wastewater Using Shells from African Cherry Seeds, Egg and Crab

Aleshinloye

Ngangsso

Adaramola

et al. 2020

AJACR

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This study investigated the potential of some agricultural wastes viz; African Star apple seed shell (ASS, plant source), crab shell (CS, animal source) and chicken egg shell (ES, animal source) as eco-friendly and low-cost biological materials for the removal of heavy metals from poultry wastewater. TS, TSS and TDS of the wastewater sample were assayed by filtration methods, chloride content by previously reported method and heavy metal contents (Zn, V, Cd, Fe, Ni, Cu, Co, Pb, Cr and Mn); were analyzed using Microwave Plasma Atomic Emission Spectrometer. The results of the solids and chloride contents of the poultry wastewater were TDS (3100 mg/L), TS (3700 mg/L), TSS (6000 mg/L) and chloride (4.7 g/L); all above the EPA permissible limits. Results of the FTIR analysis showed that ASS is an amide polymer while the CS and ES shells are mixtures of amide and carbonate polymers. Also, results of heavy metal analysis before and after adsorption showed that ASS caused removal of Zn, V, Fe, Cu, Co/ Pb and Mn by 48.27, 32.22, 49.64, 91.44, 100 and 82.39% respectively while Cd, Ni and Cr contents increased by 31, 61 and 48.3% respectively. CS showed removal of Fe, Ni/ Co/ Cr, Pb and Mn by 89.64, 100, 3.51 and 95.96% respectively while Zn, V, Cd and Cu contents increased by 1.7, 61.2, 76.1 and 68.1% respectively. Meanwhile, with ES, the contents of Zn, Fe, Ni, Cu, Cr and Mn increased by 31.56, 86.36, 100, 55.5, 45.80 and 90.33% respectively while the contents of V, Cd, Co and Pb decreased by 78.9, 86.7, 42.5 and 46.2% respectively. This study demonstrated the use of ASS, CS and ES as low- cost and eco-friendly agricultural wastes with significant potential for removal of heavy metals from wastewaters.

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The Analysis of Heavy Metal Concentrations in Eggs of Collared Flycatchers, Ficedula Albicollis (Passeriformes, Muscicapidae), and Tits, Parus Major, Parus Caeruleus (Passeriformes, Paridae), in Different Areas of North-Eastern Ukraine

Cited by 3 publications

References 16 publications

Distribution of Ixodes ricinus (Arachnida, Ixodidae) in Ukraine in the Context of Tick Hazard, and Factors Favoring its Persistence in Conditions of Fast-Going Environmental Change

Distribution of Ixodes ricinus (Arachnida, Ixodidae) in Ukraine in the Context of Tick Hazard, and Factors Favoring its Persistence in Conditions of Fast-Going Environmental Change

Sex identification of different species of wild birds using a single universal protocol to the bird sexing method based on gene polymorphism

Treatment of Poultry Wastewater Using Shells from African Cherry Seeds, Egg and Crab

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