The Analysis of Mixtures of Ethyl Alcohol, Ethyl Acetate, Acetic Acid and Water

Poznañski, S.

doi:10.1021/ja01391a007

Cited by 7 publications

(4 citation statements)

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“…The simultaneous saccharification and fermentation was carried out at 27°C for 5 days in stationary condition. At the end of incubation, the culture broth was qualitatively tested for alcohol production using the K2Cr2O7 reagent test [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential

Gupta

Samant

Sahu

2012

International Journal of Microbiology

391

197

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Eight isolates of cellulose-degrading bacteria (CDB) were isolated from four different invertebrates (termite, snail, caterpillar, and bookworm) by enriching the basal culture medium with filter paper as substrate for cellulose degradation. To indicate the cellulase activity of the organisms, diameter of clear zone around the colony and hydrolytic value on cellulose Congo Red agar media were measured. CDB 8 and CDB 10 exhibited the maximum zone of clearance around the colony with diameter of 45 and 50 mm and with the hydrolytic value of 9 and 9.8, respectively. The enzyme assays for two enzymes, filter paper cellulase (FPC), and cellulase (endoglucanase), were examined by methods recommended by the International Union of Pure and Applied Chemistry (IUPAC). The extracellular cellulase activities ranged from 0.012 to 0.196 IU/mL for FPC and 0.162 to 0.400 IU/mL for endoglucanase assay. All the cultures were also further tested for their capacity to degrade filter paper by gravimetric method. The maximum filter paper degradation percentage was estimated to be 65.7 for CDB 8. Selected bacterial isolates CDB 2, 7, 8, and 10 were co-cultured withSaccharomyces cerevisiaefor simultaneous saccharification and fermentation. Ethanol production was positively tested after five days of incubation with acidified potassium dichromate.

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Section: Methodsmentioning

confidence: 99%

Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential

Gupta

Samant

Sahu

2012

International Journal of Microbiology

391

197

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“…Short evolution of ethanol production was conducted by acidified potassium dichromate method. Both VS15 and Gd7 were grown in a 8% wheat bran medium at 37 • C and 150 rpm for 54 h. After incubation, the culture broths were inoculated by 1% saccharomyces cerevisae with the addition of 0.04% KH 2 PO 4 , 0.005% CaCO 3 , 0.002% MgSO 4 , and 0.001%NaCl, and incubated for five days [76] and the amount of ethanol produced by fermentation was estimated by the potassium dichromate method [77].…”

Section: Short Evolution Of Ethanol Production Acidified Potassium DImentioning

confidence: 99%

Comparative Analysis of Structural Variations Due to Genome Shuffling of Bacillus Subtilis VS15 for Improved Cellulase Production

Lakshmi

Drendel

Petrovski

et al. 2020

IJMS

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Cellulose is one of the most abundant and renewable biomass products used for the production of bioethanol. Cellulose can be efficiently hydrolyzed by Bacillus subtilis VS15, a strain isolate obtained from decomposing logs. A genome shuffling approach was implemented to improve the cellulase activity of Bacillus subtilis VS15. Mutant strains were created using ethyl methyl sulfonate (EMS), N-Methyl-N′ nitro-N-nitrosoguanidine (NTG), and ultraviolet light (UV) followed by recursive protoplast fusion. After two rounds of shuffling, the mutants Gb2, Gc8, and Gd7 were produced that had an increase in cellulase activity of 128%, 148%, and 167%, respectively, in comparison to the wild type VS15. The genetic diversity of the shuffled strain Gd7 and wild type VS15 was compared at whole genome level. Genomic-level comparisons identified a set of eight genes, consisting of cellulase and regulatory genes, of interest for further analyses. Various genes were identified with insertions and deletions that may be involved in improved celluase production in Gd7. Strain Gd7 maintained the capability of hydrolyzing wheatbran to glucose and converting glucose to ethanol by fermentation with Saccharomyces cerevisiae of the wild type VS17. This ability was further confirmed by the acidified potassium dichromate (K2Cr2O7) method.

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“…The mixtures were centrifuged at 5,000 g for 10 minutes to separate the hydrolysates were first supplemented with filter-sterilized salt solution (0.4 g/L KH 2 PO 4 , 0.02 g/L MgSO 4 , 0.05 g/L CaCO 3 , and 0.01 g/L NaCl) and then fermented using S. cerevisae. At the end of this incubation, the culture broth was tested for alcohol production using the K 2 Cr 2 O 7 reagent test [20].…”

Section: Bioethanol Productionmentioning

confidence: 99%

Isolation of Cellulolytic Organisms from the Gut Contents of Termites Native to Nepal and Their Utility in Saccharification and Fermentation of Lignocellulosic Biomass

Sharma¹,

Joshi²,

Bhatt³

et al. 2015

JBB

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Lignocellulose is one of the most abundant renewable materials that could serve as a source for fermentable sugars. However, its commercial deployment is limited by the fact that lignocellulose is recalcitrant and requires heroic efforts to release sugars. Although very few, there are cellulose degrading organisms in nature that can efficiently degrade the cellulosic biomass and one among them is termite. Termites are responsible for mass turnover of the cellulosic biomass and they accomplish this task by harbouring a consortium of cellulolytic and lignolytic microorganisms. In the present study, we have isolated eight bacterial and five fungal cellulose degrading organisms from the gut of a termite native to Nepal via basal culture medium enrichment with filter paper as substrate. The isolated organisms were identified as Bacillus sp., Cellulomonas sp., Enterobacter sp., and Aspergillus sp. The Congo red screening assay for cellulase production showed the largest zone of hydrolysis (38 mm) for Aspergillus sp. The carboxymethyl cellulose assay revealed that the Bacillus sp (S3B8; 0.12± 0.01 IU/ml obtained within96 hours) and Aspergillus sp. (S3F3; 0.07 ±0.01 IU/ml obtained within 168 hours) were most efficient cellulase producers. The saccharification and fermentation of lignocellulosic biomass to ethanol was successfully achieved via culturing of selected bacterial [Bacillus (S3B8)] and fungal isolates [Aspergillus (S3F3)] and then fermentation with Saccharomyces cerevisiae (S2Y1). Further characterization of these organisms and optimization of fermentation of lignocellulosic biomass is ongoing.

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The Analysis of Mixtures of Ethyl Alcohol, Ethyl Acetate, Acetic Acid and Water

Cited by 7 publications

References 0 publications

Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential

Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential

Comparative Analysis of Structural Variations Due to Genome Shuffling of Bacillus Subtilis VS15 for Improved Cellulase Production

Isolation of Cellulolytic Organisms from the Gut Contents of Termites Native to Nepal and Their Utility in Saccharification and Fermentation of Lignocellulosic Biomass

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