The analysis of oligonucleotide preparations by fractal measures

Földes-Papp, Zeno; Baumann, Gerd; Birch-Hirschfeld, Eckhard; Eickhoff, Holger; Greulich, Karl Otto; Kleinschmidt, Albrecht K.; Seliger, H.

doi:10.1002/(sici)1097-0282(19980415)45:5<361::aid-bip5>3.0.co;2-j

Cited by 9 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A problem inherent to polymer‐support synthesis is that the product is always a more or less complex mixture of the desired chain with truncated and failure sequences (Földes‐Papp et al, , and references therein). In view of the efficiency and selectivity of affinity techniques, a solution‐phase synthesis using “affinity protecting groups” would be an interesting alternative to solid‐phase preparations (Seliger, ).…”

Section: Acid‐labile Protecting Groupsmentioning

confidence: 99%

“…Recently, an estimation of capping efficiency has been available through the correlation of calculations using fractal mathematics with the results of capillary electrophoretic separation of the crude product of oligonucleotide preparations of up to 60 bases in length. For support syntheses using the common controlled‐pore glass (CPG) or polystyrene supports, the best correlation between calculated and experimentally determined chain length distributions was observed if capping was assumed to proceed with not more than 70% to 80% of the non‐elongated chain ends (Földes‐Papp et al, , ).…”

Section: Blocking Groups Labile To Nonacidic Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Protection of 5′‐Hydroxy Functions of Nucleosides

Seliger

2000

CP Nucleic Acid Chemistry

View full text Add to dashboard Cite

show abstract

Section: Acid‐labile Protecting Groupsmentioning

confidence: 99%

Section: Blocking Groups Labile To Nonacidic Conditionsmentioning

confidence: 99%

Protection of 5′‐Hydroxy Functions of Nucleosides

Seliger

2000

CP Nucleic Acid Chemistry

View full text Add to dashboard Cite

show abstract

“…The heavy and light chains of both Fab fragment and antibody are interconnected by disulfide bonds. Therefore, they exhibit an overall more rigid structure than the oligonucleotide sequences do. , …”

mentioning

confidence: 99%

Spatial Separation of Microbeads into Detection Levels by a Bioorthogonal Porous Hydrogel for Size-Selective Analysis and Increased Multiplexity

et al. 2019

View full text Add to dashboard Cite

Multiplex detection techniques are emerging within the fields of life science research and medical diagnostics where it is mandatory to analyze a great number of molecules. The detection techniques need to be highly efficient but often involve complicated and expensive fabrication procedures. Here, we present the immobilization and geometric separation of fluorescence-labeled microbeads for a multiplex detection in k levels. A compound of differently sized target molecules (DNA, proteins) is channeled into the respective detection levels by making use of a hydrogel as a size selective filter. The immobilized microbeads (10–20 μm) are considerably larger than the pores of the hydrogel network and therefore stay fixed at the well bottom and in higher elevations, respectively. Small biomolecules can diffuse through the pores of the network, whereas medium-sized biomolecules pass slower and large molecules will be excluded. Besides filtering, this method discriminates the used microbeads into k levels and thereby introduces a geometric multiplexity. Additionally, the exclusion of large entities enables the simultaneous detection of two target molecules, which exhibit the same affinity interaction. The hydrogel is formed through the combination of two macromonomers. One component is a homobifunctional polyethylene glycol linker, carrying a strained alkyne (PEG-BCN) and the second component is the azide-functionalized dendritic polyglycerol (dPG-N3). They react via the bioorthogonal strain-promoted azide alkyne cycloaddition (SPAAC). The hydrogel creates a solution-like environment for the diffusion of the investigated biomolecules all the while providing a stable, bioinert, and surface bound network.

show abstract

Introduction

Seliger

2007

Microarrays

View full text Add to dashboard Cite

Microarray technology has its roots in high-throughput parallel synthesis of biomacromolecules, combined with combinatorial science. In principle, the preparation of arrays can be performed either by in situ synthesis of biomacromolecules on solid substrates or by spotting of ex situ synthesized biomacromolecules onto the substrate surface. The application of microarrays includes spatial addressing with target (macro) molecules and screening for interactions between immobilized probe and target. The screening is simplified by the microarray format, which features a known structure of every immobilized library element. The area of nucleic acid arrays is best developed, because such arrays are allowed to follow the biosynthetic pathway from genes to proteins, and because nucleic acid hybridization is a most straightforward screening tool. Applications to genomics, transcriptomics, proteomics, and glycomics are currently in the foreground of interest; in this postgenomic phase they are allowed to gain new insights into the molecular basis of cellular processes and the development of disease.

show abstract

The analysis of oligonucleotide preparations by fractal measures

Cited by 9 publications

References 25 publications

Protection of 5′‐Hydroxy Functions of Nucleosides

Protection of 5′‐Hydroxy Functions of Nucleosides

Spatial Separation of Microbeads into Detection Levels by a Bioorthogonal Porous Hydrogel for Size-Selective Analysis and Increased Multiplexity

Introduction

Contact Info

Product

Resources

About