The Analysis of Smad3 Gene Mutations in Patients with Osteoarthritis

Soyöz, Mustafa; Özçelik, Nurten; Calapoğlu, Nilüfer Şahin; Akkuş, Selami; İlgün, Erdem

doi:10.5336/medsci.2009-16002

Cited by 1 publication

(1 citation statement)

References 10 publications

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“…If any deviation is shown, it must be evaluated with the case II transport mechanism. [56][57][58] Further study might be conducted in order to precisely determine the release kinetics of our CPBH. Zero and first-order kinetics and their goodness of fit analysis according to Higuchi and Hixon Crowell equivalents may be an issue for another experimental study.…”

Section: Discussionmentioning

confidence: 99%

A Novel Designed Chitosan Based Hydrogel Which is Capable of Consecutively Controlled Release of TGF-Beta 1 and BMP-7

Yılmaz¹,

Gokay²,

Gökçe³

et al. 2013

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Both Bone Morphogenetic Protein 7 (BMP-7) and Transforming Growth Factor Beta 1 (TGF-β1) have stimulating effects on chondrocyte proliferation. These growth factors act at different time points of the biological repair process. We aimed to design a hydrogel, which would enable consecutive controlled release of the growth factors. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : We designed a chitosan particle impregnated Poly (vinylalcohol) (PVA)-borax hydrogel (CPBH). CPBH allowed controlled release of BMP-7 and TGF-β1 at different time points. Hydrogel structure was analyzed by Scanning Electron Microscope (SEM). The release kinetics of Growth Factors (GFs) were determined with enzyme-linked immunosorbent assay (ELISA) and UV-spectrophotometer. Chondrocyte viability and toxicity were tested through CellTiter 96 ® Non-Radioactive Cell Proliferation (MTS) ELISA. R Re es su ul lt ts s: : The designed hydrogel showed high swelling and mucoadhesion characteristics under acidic conditions. CPBH released BMP-7 first and rapidly and TGF-β1 consecutively and slowly. It also allowed controlled release of protein/peptide based drugs for 21 days without altering their bioactive properties. At the end of 21 days, 82.62% of BMP-7 and 98.34% of TGF-β1 were consecutively released. The difference between the groups was significant (for TGF-β1, p<0.05 and for BMP-7, p<0.001). C Co on nc cl lu us si io on n: : The controlled and slow release of growth factors has been shown to be beneficial on cartilage regeneration. As it is not cytotoxic, we suggest that this hydrogel might be used in medical and pharmaceutical applications areas. K Ke ey y W Wo or rd ds s: : Delayed-action preparations; hydrogels; TGF-β1 protein, human; bone morphogenetic protein 7 Ö ÖZ ZE ET T A Am ma aç ç: : Kemik Morfojenik Proteini-7 (BMP-7) ve Transforme edici Büyüme Faktörü Beta-1 (TGF-β1)'in kıkırdak hücreleri üzerinde prolifere edici etkileri bulunmaktadır. Bu büyüme faktörlerinin etkisi biyolojik tamir sürecinin farklı zamanlarında gerçekleşmektedir. Bu çalışmada, büyüme faktörlerinin ardışık ve kontrollü olarak salınımını gerçekleştirecek bir hidrojel tasarlamayı amaçladık. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Bu amaçla kitosan emdirilmiş polivinil alkol (PVA)-boraks hidrojeli (CPBH) tasarlandı. Bu hidrojel BMP-7 ve TGF-β1'in farklı zamanlarda kontrollü salınımını sağladı. Tasarladığımız hidrojelin yüzey yapısı taramalı elektron mikroskobu (SEM) ile değerlendirildi. Büyüme faktörlerinin salım kinetiği enzim aracılı immün deney (ELISA) ve ultraviyole (UV)spektrofotometre ile saptandı. Kondrosit canlılığı ve toksisite testi "CellTiter 96 ® Non-Radioactive Cell Proliferation (MTS)" ELISA yöntemiyle değerlendirildi. B Bu ul lg gu ul la ar r: : Tasarlanan hidrojel, asidik ortam koşullarında büyük oranda şişme ve mukoadezyon özellikleri sergiledi. CPBH, önce hızlı bir biçimde BMP-7'nin, sonra da yavaş olarak TGF-β1'in salınımını ardışık olarak gerçekleştirdi. İçerisindeki p...

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Section: Discussionmentioning

confidence: 99%