The analysis on the expression of gasotransmitters in early trauma patients

Abstract

Objective: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H 2 S) were endogenously-generated molecules gas. They owned important biological activity and participated in many pathophysiological processes. This study aimed to examine the levels of three gasotransmitters in the early phase of trauma patients. Method: Blood samples were collected from 60 trauma patients and ten healthy volunteers. Concentration of serum iNOS and HO-1 were analyzed by enzyme linked immunosorbent assay and plasma H 2 S was determined by colorimetric method. Meanwhile, the occurrence of multiple organ dysfunction syndrome (MODS) was also monitored. Results: The levels of iNOS, HO-1 and endogenous H 2 S in the patients group were significantly different from the healthy control group, and the difference was more obvious with the increase of ISS score. iNOS levels were positively correlated with ISS scores and blood lactic acid values, and HO-1 and endogenous H2S were negatively correlated with ISS scores and blood lactic acid values. Of 60 trauma patients, eight (13.33%) developed MODS. The level of iNOS in the MODS group was higher than that in non-MODS group, while HO-1 and H 2 S were significant lower in the MODS group. Conclusion: The three gasotransmitters participated in systemic inflammatory responses during early trauma and could be used as important indicators for trauma severity. Their measurements were meaningful for evaluating the severity and prognosis of trauma.

Microfluidics-based rapid measurement of nitrite in human blood plasma

“…7 It interacts with superoxide ions and produces toxic peroxynitrite that can damage cells. 4 It is also involved in neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). 1 Conditions such as trauma, shocks, and infections produce higher levels of NO, and in the experimental sepsis model, an elevated NO concentration was observed.…”

“…2 Studies have revealed that gasotransmitters control the generation of adenosine triphosphate (ATP), insulin sensitivity, and metabolism of glucose and lipids, and hence abnormal production of gasotransmitters is responsible for different metabolic diseases such as diabetes and obesity. 3 Furthermore, gasotransmitters are essential signalling molecules in inflammatory reactions, 4 and can also exhibit different protective roles against oxidative stress. 1 In general, gasotransmitters have a beneficial role if produced at low concentrations but lead to toxicity at higher concentrations.…”