The analytical determination of polyphosphates in food: A point-to-point comparison between direct ion chromatography and indirect photometry

Iammarino, Marco; Haouet, Naceur; Taranto, Aurelia Di; Berardi, Giovanna; Benedetti, Ferdinando; Bella, Sara Di; Chiaravalle, Antonio Eugenio

doi:10.1016/j.foodchem.2020.126937

Cited by 10 publications

(9 citation statements)

References 18 publications

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“…The comparison among different approaches for a specific analytical determination is essential when a laboratory aims at implementing a new procedure. Point-to-point comparisons, mainly based on the available validation parameters and the application to commercial/real samples, are extremely useful for evaluating the pros/cons of each approach, as well as relating to the specific need, ability, equipment, and expertise of a laboratory [13,31,32]. However, to date, there is no available comparison regarding screening and confirmatory approaches in mycotoxin determination.…”

Section: Comparison Between the Elisa And Uplc/fld Methodsmentioning

confidence: 99%

ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals

et al. 2021

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T-2 and HT-2 toxins are secondary metabolites of various species of Fusarium. These molecules can have high potential toxic effects for human and animal health. In this work, ELISA and ultra performance liquid chromatography with fluorescence detection (UPLC/FLD) were implemented and validated as screening and confirmatory tests for the detection of these two toxins in cereal samples. The developed methods were tested by analyzing 100 samples of cereals by ELISA screening for reducing costs and analysis time and then using UPLC/FLD for confirmation purposes. Both methods met the performance criteria for sensitivity, linearity, selectivity, precision, and ruggedness, as reported in the European Decision No. 2002/657/EC and in Regulation (EC) No. 401/2006. The correlation between ELISA and UPLC/FLD approaches showed good results (r = 0.9056), confirming that these two techniques should be considered to be complementary in the official control activities of cereal and derived products.

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Section: Comparison Between the Elisa And Uplc/fld Methodsmentioning

confidence: 99%

ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals

et al. 2021

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“…Despite satisfactory results of HPIEC–SCD analysis reported recently [ 19 , 25 ], demand for more specific methods with satisfactory robustness has arisen due to the well-known (poly)phosphate dependence on the sample matrix type. Nevertheless, HPIEC with SCD remains a priority choice for the analysis of short-chain phosphate anions, as it was proven to be very sensitive and precise for the ortho-, pyro-, trimeta- and triphosphate, although its main drawback is that it cannot identify polymeric structures with more than four orthophosphate units [ 19 , 24 , 25 ]. However, this problem has been successfully resolved with the HPIEC–SCD protocol described above [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…Regulation 1169/2011 provides for transparent labelling and improves consumer protection against common fraud [ 6 ]. In order to verify conformity with legislation and to comply with correct labelling, both producers and inspection authorities have to (1) know the natural composition of food material and if there is any overlap with exogenously added substances, and (2) adopt more specific analytical techniques, e.g., IC with different detection modes [ 19 , 26 ]. Hence, this study analysed the undeclared food samples of animal origin belonging to the three main categories.…”

Section: Resultsmentioning

confidence: 99%

“…From an analytical point of view, the determination of (poly)phosphates has always represented a major problem for bodies responsible for controlling food of animal origin. In fact, the reference spectrophotometric method [ 18 ] does not provide the direct determination of (poly)phosphates but determines only the so-called “relevant phosphorus”, i.e., phosphorus content that, in relation to the protein content, can be considered adequate [ 19 ]. This technique is non-specific and can lead to overestimates if other phosphorous-containing additives are present [ 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…This technique is non-specific and can lead to overestimates if other phosphorous-containing additives are present [ 20 , 21 , 22 ]. In Italy, this issue has been extensively addressed, making it possible to compare the current spectrophotometric reference method with those introduced recently (e.g., high-performance ion-exchange chromatography with suppressed conductometry, HPIEC–SCD) [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Undeclared (Poly)phosphates Detection in Food of Animal Origin as a Potential Tool toward Fraud Prevention

Pavlović

Cesare

Longo

et al. 2021

Foods

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(Poly)phosphates are approved as water-preserving and emulsifying agents that improve the appearance and consistency of many food products. The labelling of added (poly)phosphates is essential for protecting vulnerable population groups and to prevent unfair trade practices resulting in economic fraud. The problems with (poly)phosphates’ utilisation concerns both analytical and legislative issues, such as: (1) their straightforward detection; (2) excessive addition altering freshness perception and misleading consumers; (3) uncontrolled usage increasing foodstuff weight; (4) application in products where they are not permitted; and (5) no indication on the label. Bearing all these issues in mind, the main purpose of this study was the quantification and screening of the (poly)phosphates profile in meat, marine and dairy products (160 samples), of which 43 were without declared (poly)phosphate treatment. Analysis was completed by high-performance ion-exchange chromatography either with conductometric detection or coupled to Q-Exactive Orbitrap high-resolution mass spectrometry. Although the (poly)phosphates profiles varied greatly according to species and processing type, the following criteria for detection of illicit treatment were established: high orthophosphate level, quantified short-chain (poly)phosphate anions and the presence of long-chain forms. In conclusion, the instrumental platforms used in this study can be recommended to inspection bodies as reliable methods for the detection of food adulteration with (poly)phosphates.

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Simple, fast, and simultaneous determination of orthophosphate, pyrophosphate, and tripolyphosphate by capillary electrophoresis with capacitively coupled contactless conductivity detection

Barboza,

Rocha,

de Jesus

2024

Electrophoresis

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This work describes a novel analytical method using capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C4D) for simultaneous, simple, and rapid determination of three inorganic phosphates (orthophosphate, pyrophosphate, and tripolyphosphate) widely used as food additives and in pharmaceutical formulations. A background electrolyte composed of 0.5 mol L–1 acetic acid provided fast separation (around 3.0 min) and good separation efficiency and peak resolution. Linearity in the concentration range of 10–500 mg L–1 was confirmed by the coefficients of determination (R2) higher than 0.99. The limits of detection varied from 0.41 to 0.58 mg L–1. The accuracy of the proposed method was assessed by recovery tests conducted at three concentration levels in tap water samples, food, and personal hygiene products. Recovery values varying from 81% to 118% were achieved, indicating an acceptable accuracy. The proposed CE‐C4D successfully determined the three inorganic phosphates in the analyzed samples.

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The analytical determination of polyphosphates in food: A point-to-point comparison between direct ion chromatography and indirect photometry

Cited by 10 publications

References 18 publications

ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals

ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals

Undeclared (Poly)phosphates Detection in Food of Animal Origin as a Potential Tool toward Fraud Prevention

Simple, fast, and simultaneous determination of orthophosphate, pyrophosphate, and tripolyphosphate by capillary electrophoresis with capacitively coupled contactless conductivity detection

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