The anatomical boundary of the rat claustrum

Dillingham, Christopher M.; Mathiasen, M.; Frost, Bethany E.; Lambert, Marie A. C.; Bubb, Emma J.; Jankowski, Maciej M.; Aggleton, John P.; O’Mara, Shane M.

doi:10.1101/596304

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…The claustrum was studied using a series of coronal brain sections from across the rostrocaudal axis (Figure 1a), giving high spatial resolution in the dorsoventral and mediolateral axes (see Materials and (Dillingham et al, 2019;Druga, Chen, & Bentivoglio, 1993;Mathur, Caprioli, & Deutch, 2009;Wang et al, 2017;White et al, 2017;Zingg et al, 2018). Comparing these two markers in dorsoventral, mediolateral, and rostrocaudal axes, showed a highly correlated spatial overlap, indicating that both methods identify a common region of the claustrum (Figure 1a-c).…”

Section: Resultsmentioning

confidence: 99%

“…First, we required a consistent anatomical landmark to spatially register claustrum neurons across experiments. Parvalbumin (PV) neuropil labeling, and retrograde tracing from the retrosplenial cortex (RSP) have both been used to topographically locate the claustrum (Dillingham et al, 2019;Druga, Chen, & Bentivoglio, 1993;Mathur, Caprioli, & Deutch, 2009;Wang et al, 2017;White et al, 2017;Zingg et al, 2018). Comparing these two markers in dorsoventral, mediolateral, and rostrocaudal axes, showed a highly correlated spatial overlap, indicating that both methods identify a common region of the claustrum (Figure 1a and lateral entorhinal cortex (ENTl) ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

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Topographic gradients define the projection patterns of the claustrum core and shell in mice

Marriott

Alison

Zahacy

et al. 2020

J of Comparative Neurology

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The claustrum is densely connected to the cortex and participates in brain functions such as attention and sleep. Although some studies have reported the widely divergent organization of claustrum projections, others describe parallel claustrocortical connections to different cortical regions. Therefore, the details underlying how claustrum neurons broadcast information to cortical networks remain incompletely understood. Using multicolor retrograde tracing we determined the density, topography, and co-projection pattern of 14 claustrocortical pathways, in mice. We spatially registered these pathways to a common coordinate space and found that the claustrocortical system is topographically organized as a series of overlapping spatial modules, continuously distributed across the dorsoventral claustrum axis. The claustrum core projects predominantly to frontal-midline cortical regions, whereas the dorsal and ventral shell project to the cortical motor system and temporal lobe, respectively. Anatomically connected cortical regions receive common input from a subset of claustrum neurons shared by neighboring modules, whereas spatially separated regions of cortex are innervated by different claustrum modules. Therefore, each output module exhibits a unique position within the claustrum and overlaps substantially with other modules projecting to functionally related cortical regions. Claustrum inhibitory cells containing parvalbumin, somatostatin, and neuropeptide Y also show unique topographical distributions, suggesting different output modules are controlled by distinct inhibitory circuit motifs. The topographic organization of excitatory and inhibitory cell types may enable parallel claustrum outputs to independently coordinate distinct cortical networks.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Topographic gradients define the projection patterns of the claustrum core and shell in mice

Marriott

Alison

Zahacy

et al. 2020

J of Comparative Neurology

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“…Currently, there are four parcellation schemes for the mouse CLA. In one, the CLA is split into the dorsal and ventral subdivisions underneath the gustatory and agranular insular areas, respectively (Dillingham et al, 2019;Paxinos and Franklin's, 2019). In a second scheme, the CLA is delineated largely underneath the gustatory, agranular insular, and visceral areas, but not subdivided.…”

Section: Introductionmentioning

confidence: 99%

Regional and cell type-specific afferent and efferent projections of the mouse claustrum

Wang

Xie

et al. 2022

Preprint

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The claustrum (CLA) is a conspicuous subcortical structure interconnected with cortical and subcortical regions. However, its regional anatomy and cell-type-specific connections in the mouse remain largely undetermined. Here, we accurately delineated the boundary of the mouse CLA and quantitatively investigated its inputs and outputs brain-wide using anterograde and retrograde viral tracing and fully reconstructed single claustral principal neurons. At a population level, the CLA reciprocally connects with all isocortical modules. It also receives inputs from at least 35 subcortical structures but sends projections back to only a few of them. We found that cell types projecting to the CLA are differentiated by cortical areas and layers. We classified single CLA principal neurons into at least 9 cell types that innervate the diverse sets of functionally linked cortical targets. Axons of interneurons within the CLA arborize along almost its entire anteroposterior extent. Together, this detailed wiring diagram of the cell-type-specific connections of the mouse CLA lays a foundation for studying its functions.

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“…To determine the mechanistic contributions of the claustrum to these putative functions, it is essential to understand both the intrinsic organization of claustrum neurons, as well as how this organization relates to connectivity and function (Edelstein and Denaro, 2004). However, such an interpretation is challenged by the fact that even the precise anatomical boundaries of the claustrum are a matter of debate (Dillingham et al, 2019;Smith et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Spatially patterned excitatory neuron subtypes and circuits within the claustrum

Erwin

Sullivan

et al. 2021

Preprint

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The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow region that primarily projects to the neocortex, whereas circuit-based approaches suggest a broader region embedding neocortical and other neural circuits. Here, we took a bottom up, cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum is comprised of two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential projection targets. Thus, the claustrum is comprised of excitatory neuron subtypes with distinct molecular and circuit properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum.

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The anatomical boundary of the rat claustrum

Cited by 4 publications

References 39 publications

Topographic gradients define the projection patterns of the claustrum core and shell in mice

Topographic gradients define the projection patterns of the claustrum core and shell in mice

Regional and cell type-specific afferent and efferent projections of the mouse claustrum

Spatially patterned excitatory neuron subtypes and circuits within the claustrum

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