The anatomy of the tRNA-rRNA region of the Neurospora crassa mitochondrial DNA

Vries, H. de; Jonge, Jenny C. de; Bakker, H.; Kroon, A.M.

doi:10.1093/nar/6.5.1791

Cited by 34 publications

(10 citation statements)

References 19 publications

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“…This finding suggests that the production of defective mtDNAs in Neurospora is nonrandom, with a preferred mechanism leading to retention of EcoRI-1, -4, and -6, the segment that contains both rRNA genes and the large majority of tRNA genes (11)(12)(13)(14)(15). By (1980) identified so far retain EcoRI-6 (and possibly adjoining sequences in EcoRI-4) and (ii) a 1.4-MDal sequence at the boundary of EcoRI-4 and -6 has been found to be amplified by tandem reiterations in some mutant strains (6).…”

Section: Resultsmentioning

confidence: 99%

Deletion mutants of Neurospora crassa mitochondrial DNA and their relationship to the "stop-start" growth phenotype.

Bertrand

Collins

Stohl

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

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ABSTRACT"Stoppers" are a class of Neurospora crassa extranuclear mutants characterized by gross deficiencies of cytochromes b and aa3 and an unusual growth phenotype which involves irregular periods of growth and nongrowth. In the present work, mtDNAs from all four stopper mutants were found to contain deletions or insertions detectable by restriction enzyme analysis. [stp] mtDNA consists predominantly of defective molecules which retain a 16-megadalton segment 4, [stp] mtDNA (i.e., EcoRI-1, 4, and -6). The findings suggest that production of defective mtDNAs in Neurospora is nonrandom with a preferred mechanism leading to retention of this segment. It may be significant that the retained segment contains both mitochondrial rRNA genes and most mitochondrial tRNA genes. These deletion mutants may provide a tool for genetic mapping of Neurospora mtDNA."Stoppers" are a class of Neurospora crassa extranuclear mutants distinguished by an unusual growth pattern on solid media: in contrast to wild-type strains which grow by continuous extension of hyphal tips, stopper mutants alternate between periods of growth and nongrowth, either of which may last for a few hours or several days (1-5). Stopper mutants are also characterized by gross alterations in mitochondrial phenotype including severe deficiencies of cytochromes b and aa3 (1-5). These findings suggest that the stopper phenotype results from a mutation in mtDNA, but the relationship between the mitochondrial defect and the growth characteristics has remained obscure.Recently, in the course of studying variant Neurospora mtDNAs which contain tandem reiterations, Mannella et al. (6)

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Section: Resultsmentioning

confidence: 99%

Deletion mutants of Neurospora crassa mitochondrial DNA and their relationship to the "stop-start" growth phenotype.

Bertrand

Collins

Stohl

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

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“…One yeast mitochondrial tRNA (tRNA,h) is transcribed from the strand opposite that from which the other tRNA's, the rRNA's, and all of the mRNA's are transcribed (278). In N. crassa (127) and A. nidulans (248), tRNA genes are mostly clustered in two regions of the mitochondrial genome, one between the rRNA genes and another following the gene for the large rRNA. The rRNA-tRNA gene region in Aspergillus (10 kbp) appears to be a condensed version of the corresponding Neurospora region (17 kbp), which contains about the same number of tRNA genes but longer intron, spacer, and rRNA sequences (248,269).…”

Section: Genetic Function Of Mitochondrialmentioning

confidence: 99%

Has the endosymbiont hypothesis been proven?

Gray¹,

Doolittle²

1982

Microbiological Reviews

327

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“…50], are nevertheless part of the same transcriptional unit [53,54]. In animal mitochondria, the genes for the large and small subunit rRNAs are adjacent [10][11][12], but in some other mitochondrial systems, notably Neurospora [6][7][8], yeast [9], and Tetrahymena [55], the genes for the large and small subunit rRNAs are far apart [> 5 kbp]. However, in none of these mitochondrial systems has a 5S rRNA molecule been described.…”

Section: Wheat Mitochondrial Genomementioning

confidence: 99%

“…3B), where there were four labeled fragments 10-15 times the size of an rRNA structural gene--each fragment approaching or even exceeding the size of the entire animal mitochondrial genome! Admittedly, the presence of long intervening sequences within rRNA structural genes [6][7][8]57] might be expected to increase the probability of a gene being distributed between two or more restriction fragments, as, for example, in the case of the ovalbumin gene [58]. However, the possibility of internal cleavage is not compatible with the fact that both 18S and 5S rRNAs hybridized to all of the scane fragments in Eco RI and Sal I digests of wheat mtDNA (Fig.…”

Section: Wheat Mitochondrial Genomementioning

confidence: 99%

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Organization and expression of the mitochondrial genome of plants I. The genes for wheat mitochondrial ribosomal and transfer RNA: evidence for an unusual arrangement

1980

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We show here that mitochondrial-specific ribosomal and transfer RNAs of wheat (Triticum vulgare Vill. [Triticum aestivum L.] var. Thatcher) are encoded by the mitochondrial DNA (mtDNA). Individual wheat mitochondrial rRNA species (26S, 18S, 5S) each hybridized with several mtDNA fragments in a particular restriction digest (Eco RI, Xho I, or Sal I). In each case, the DNA fragments to which 18S and 5S rRNAs hybridized were the same, but different from those to which 26S rRNA hybridized. From these results, we conclude that the structural genes for wheat mitochondrial 18S and 5S rRNAs are closely linked, but are physically distant from the genes for wheat mitochondrial 26S rRNA. This arrangement of rRNA genes is clearly different from that in prokaryotes and chloroplasts, where 23S, 16S and 5S rRNA genes are closely linked, even though wheat mitochondrial 18S rRNA has previously been shown to be prokaryotic in nature. The mixed population of wheat mitochondrial 4S RNAs (tRNAs) hybridized with many large restriction fragments, indicating that the tRNA genes are broadly distributed throughout the mitochondrial genome, with some apparent clustering in regions containing 18S and 5S rRNA genes.

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The anatomy of the tRNA-rRNA region of the Neurospora crassa mitochondrial DNA

Cited by 34 publications

References 19 publications

Deletion mutants of Neurospora crassa mitochondrial DNA and their relationship to the "stop-start" growth phenotype.

Deletion mutants of Neurospora crassa mitochondrial DNA and their relationship to the "stop-start" growth phenotype.

Has the endosymbiont hypothesis been proven?

Organization and expression of the mitochondrial genome of plants I. The genes for wheat mitochondrial ribosomal and transfer RNA: evidence for an unusual arrangement

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