The Angiogenesis Inhibitor TNP-470 Reduces the Growth Rate of Human Neuroblastoma in Nude Rats

Noninvasive estimation of tumour viability in a xenograft model of human neuroblastoma with proton magnetic resonance spectroscopy (1H MRS)

“…We could, however, detect higher ML/Cho ratios in relation to tumour volume in treated tumours ( Figure 5A). Concomitantly, marked histological changes were seen in TNP-470-treated tumours ( Figure 4E,F) similar to those previously reported (Wassberg et al, 1997). Taken together, our observations suggest that the higher ML/Cho ratios detected after short-term TNP-470 treatment indicate early antitumour effects as reflected in the histological appearance.…”

“…In our present study, neither creatine nor NAA signals were unequivocally accessible for spectroscopic analysis, nor were we able to show any correlation between ML/Cho ratio and proliferative activity . The antiangiogenic compound TNP-470 is an inhibitor of endothelial cells (Castronovo and Belotti, 1996) and has been shown to reduce the growth of neuroblastoma xenografts, causing increased necrosis and apoptosis (Wassberg et al, 1997;Katzenstein et al, 1999). In experimental studies of antiangiogenic drugs, tumour volume measurements have generally been employed for in vivo assessment of treatment efficacy.…”

“…Tumours were measured with a digital calliper every other day and tumour volume was calculated by length  width 2  0.44 (Wassberg et al, 1997). Tumours were allowed to reach a maximum length of 25 mm.…”

“…Animals in the treatment group received the antiangiogenic compound TNP-470 (kindly provided by Dr Rolf Christofferson, Uppsala) 10 mg kg À1 s.c. every other day for 10 days (Wassberg et al, 1997). Control animals received no treatment.…”

Reproducibility of total choline/water ratios in mouse U87MG xenograft tumors by 1H‐MRS

“…Body weights and tumor size measured by calipers were obtained twice per week beginning on day 7 after implantation. Tumor volume was calculated as length  width 2  0.44 (24). Animals with an average tumor volume of %150 mm 3 (determined by caliper measurement) on the first day of the MRI/MRS scans (12 or 13 days after cell implantation) were enrolled in the study (n ¼ 36).…”