The Angiogenic Paracrine Potential of Mesenchymal Stem Cells

Rezaie, Jafar; Heidarzadeh, Morteza; Hassanpour, Mehdi; Amini, Hassan; Shokrollahi, Elhameh; Ahmadi, Mahdi; Rahbarghazi‬, Reza

doi:10.5772/intechopen.84433

Cited by 16 publications

(15 citation statements)

References 130 publications

(139 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well documented that MSC are stimulating the environment and other cells through the paracrine activ- ity via production of growth factors, mediators and other bioactive molecules, included in a conditioned medium (Humenik et al 2019;Rezaie et al 2019;Maacha et al 2020). Previous study focused on proteomic analyses of MSCCM from adipose tissue and umbilical cord showed, that there are differences in composition of secretomes from these sources (Shin et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Impact of mesenchymal stem cells derived conditioned media on neural progenitor cells

Humeník¹,

Jego²,

Hornakova³

et al. 2021

gpb

View full text Add to dashboard Cite

Neurodegenerative diseases are common problem for companion animals. Due to the limited ability of injured axons to regenerate, innovative therapies combined with rehabilitation have been applied and evaluated. Among them, stem cells and their conditioned media implantation, which can ameliorate damaged tissue has been suggested as a promising treatment strategy. The main goal of our study was to characterize mesenchymal stem cells (MSC) derived from canine adipose tissue (AT-MSC) and umbilical cord (UC-MSC) and analyse effect of their conditioned media (CM) on neurite outgrowth of neural progenitor cells isolated from the brain cortex of neonatal rats. MSC from both sources showed high osteogenic and chondrogenic potential and expression of CD90 and CD29. Furthermore, both UC-MSCCM and AT-MSCCM stimulated neurite growth. Interestingly, this effect was more pronounced with UC-MSCCM when compared to AT-MSCCM in vitro, which may be related to the different content of neurotrophic factors included in the CM.

show abstract

Section: Discussionmentioning

confidence: 99%

Impact of mesenchymal stem cells derived conditioned media on neural progenitor cells

Humeník¹,

Jego²,

Hornakova³

et al. 2021

gpb

View full text Add to dashboard Cite

show abstract

“…Along with ECs, mesenchymal stem cells (MSCs) play a key role in the angiogenic process by facilitating blood vessel stabilization and maturation. [ 143 , 144 ] Specifically, MSCs actively participate in angiogenesis via secretion of proangiogenic factors (i.e., VEGF, MCP‐1, IL‐6, etc.) and MSC‐released paracrine factors are responsible for activation of the ECs angiogenic functions.…”

Section: Vascularization Approaches For Physiologically Relevant 3d Modelsmentioning

confidence: 99%

“…and MSC‐released paracrine factors are responsible for activation of the ECs angiogenic functions. [ 143 , 145 ] Given their multipotency, MSCs also induce direct differentiation and cell–cell interactions with endothelial lineage, suggesting that MSCs could be used to facilitate vascularization in spheroids and organoids. [ 144 ] For example, spheroids fabricated using only MSCs was found to generate vascularized spheroids with improved osteogenic differentiation and bone formation.…”

Section: Vascularization Approaches For Physiologically Relevant 3d Modelsmentioning

confidence: 99%

In Vitro Strategies to Vascularize 3D Physiologically Relevant Models

et al. 2021

View full text Add to dashboard Cite

Vascularization of 3D models represents a major challenge of tissue engineering and a key prerequisite for their clinical and industrial application. The use of prevascularized models built from dedicated materials could solve some of the actual limitations, such as suboptimal integration of the bioconstructs within the host tissue, and would provide more in vivo-like perfusable tissue and organ-specific platforms. In the last decade, the fabrication of vascularized physiologically relevant 3D constructs has been attempted by numerous tissue engineering strategies, which are classified here in microfluidic technology, 3D coculture models, namely, spheroids and organoids, and biofabrication. In this review, the recent advancements in prevascularization techniques and the increasing use of natural and synthetic materials to build physiological organ-specific models are discussed. Current drawbacks of each technology, future perspectives, and translation of vascularized tissue constructs toward clinics, pharmaceutical field, and industry are also presented. By combining complementary strategies, these models are envisioned to be successfully used for regenerative medicine and drug development in a near future.

show abstract

“…Since MSCs regulate vascular remodeling and angiogenesis (21), we assessed the proangiogenic activity of conditioned medium (CM) from B. henselae-infected MSCs. To this end, CM from uninfected or B. henselae-infected MSC cultures were tested in a scratch wound healing assay using HUVECs.…”

Section: B Henselae-infected Mscs Promote Angiogenesis and Infection Of Endothelial Cellsmentioning

confidence: 99%

Bartonella henselae Persistence within Mesenchymal Stromal Cells Enhances Endothelial Cell Activation and Infectibility That Amplifies the Angiogenic Process

et al. 2021

View full text Add to dashboard Cite

Some bacterial pathogens can manipulate the angiogenic response, suppressing or inducing it for their own ends. In humans, B. henselae is associated with cat-scratch disease and vasculoproliferative disorders such as bacillary angiomatosis and bacillary peliosis. Although endothelial cells (ECs) support the pathogenesis of Bartonella , the mechanisms by which Bartonella induces EC activation are not completely clear, as well as the possible contribution of other cells recruited at the site of infection. Mesenchymal stromal cells (MSCs) are endowed with angiogenic potential and play a dual role in infections exerting antimicrobial properties but also acting as a shelter for pathogens. Here we delved into the role of MSCs as reservoir of Bartonella and modulator of EC functions. B. henselae readily infected MSCs and survived in perinuclear bound vacuoles for up to 8 days. Infection enhanced MSC proliferation and the expression of EGFR, TLR2 and NOD1, proteins that are involved in bacterial internalization and cytokine production. Secretome analysis revealed that infected MSCs secreted higher levels of the proangiogenic factors VEGF, FGF-7, MMP-9, PIGF, serpin E1, TSP-1, uPA, IL-6, PDGF-D, CCL5 and CXCL8. Supernatants from B. henselae -infected MSCs increased the susceptibility of ECs to B. henselae infection and enhanced EC proliferation, invasion and reorganization in tube-like structures. Altogether, these results candidate MSCs as a still underestimated niche for B. henselae persistent infection and reveal a MSC-EC crosstalk that may contribute to exacerbate bacterial-induced angiogenesis and granuloma formation.

show abstract

The Angiogenic Paracrine Potential of Mesenchymal Stem Cells

Cited by 16 publications

References 130 publications

Impact of mesenchymal stem cells derived conditioned media on neural progenitor cells

Impact of mesenchymal stem cells derived conditioned media on neural progenitor cells

In Vitro Strategies to Vascularize 3D Physiologically Relevant Models

Bartonella henselae Persistence within Mesenchymal Stromal Cells Enhances Endothelial Cell Activation and Infectibility That Amplifies the Angiogenic Process

Contact Info

Product

Resources

About