The anion exchanger PAT-1 (Slc26a6) does not participate in oxalate or chloride transport by mouse large intestine

Whittamore, Jonathan M.; Hatch, Marguerite

doi:10.1007/s00424-020-02495-x

Cited by 7 publications

(4 citation statements)

References 92 publications

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“…Unlike the distal ileum, PAT1 does not contribute to the secretory flux of oxalate in murine cecum, at least under basal conditions (Whittamore & Hatch, 2020). However, DRA has been shown to mediate the oxalate absorptive flux in this segment (Freel et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…In the distal colon, a significant portion of oxalate absorption has been shown to occur through DRA under symmetrical, short‐circuit conditions in vitro (Freel et al, 2013), and similar to the cecum, there does not appear to be a role for PAT1 or any other DIDS‐sensitive anion transporter (Whittamore & Hatch, 2020). However, oxalate secretion in the murine distal colon is dependent on carbonic anhydrase (Whittamore et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Individual tissue sections (~ 2 cm long each) of the distal ileum (up to 4 cm proximally from the ileocecal valve), cecum, and distal colon (up to 4 cm proximally from the peritoneal border) were then prepared by cutting along the mesenteric line and mounting on Ussing chamber sliders (P2304; Physiologic Instruments, San Diego, CA, USA) to expose 0.3 cm 2 of surface area. These intestinal segments were chosen and left intact to match the segments examined in previous studies that identified DRA and PAT1 as oxalate transporters (Freel et al, 2006(Freel et al, , 2013Whittamore & Hatch, 2020). Furthermore, sodium transport across the murine distal ileum, cecum, and distal colon has only been previously measured in intact tissues (Charney et al, 2004;Homaidan et al, 1999).…”

Section: Transepithelial Flux Experimentsmentioning

confidence: 99%

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The role of NHE3 (Slc9a3) in oxalate and sodium transport by mouse intestine and regulation by cAMP

2021

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Transepithelial Flux Experimentsmentioning

confidence: 99%

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The role of NHE3 (Slc9a3) in oxalate and sodium transport by mouse intestine and regulation by cAMP

2021

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“…In a mouse model of chronic kidney disease, SLC26A6-mediated intestinal oxalate secretion is essential to reduce the body burden of oxalate ( 98 ). A net secretion of oxalate exists in the distal colon of mice, but it does not require the involvement of SLC26A6 and may have other unknown transporters ( 99 ). SLC26A6 overexpression in the kidney increases urinary oxalate excretion and promotes stone formation, whereas overexpression of SLC26A6 in the intestine increases intestinal oxalate secretion, reduces urinary oxalate excretion, and plays a protective role in stone formation.…”

Section: Oxalate Excretionmentioning

confidence: 99%

Oxalate as a potent promoter of kidney stone formation

et al. 2023

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Kidney stones are among the most prevalent urological diseases, with a high incidence and recurrence rate. Treating kidney stones has been greatly improved by the development of various minimally invasive techniques. Currently, stone treatment is relatively mature. However, most current treatment methods are limited to stones and cannot effectively reduce their incidence and recurrence. Therefore, preventing disease occurrence, development, and recurrence after treatment, has become an urgent issue. The etiology and pathogenesis of stone formation are key factors in resolving this issue. More than 80% of kidney stones are calcium oxalate stones. Several studies have studied the formation mechanism of stones from the metabolism of urinary calcium, but there are few studies on oxalate, which plays an equally important role in stone formation. Oxalate and calcium play equally important roles in calcium oxalate stones, whereas the metabolism and excretion disorders of oxalate play a crucial role in their occurrence. Therefore, starting from the relationship between renal calculi and oxalate metabolism, this work reviews the occurrence of renal calculi, oxalate absorption, metabolism, and excretion mechanisms, focusing on the key role of SLC26A6 in oxalate excretion and the regulatory mechanism of SLC26A6 in oxalate transport. This review provides some new clues for the mechanism of kidney stones from the perspective of oxalate to improve the understanding of the role of oxalate in the formation of kidney stones and to provide suggestions for reducing the incidence and recurrence rate of kidney stones.

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Oxalate Flux Across the Intestine: Contributions from Membrane Transporters

Whittamore

Hatch

2021

Comprehensive Physiology

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Epithelial oxalate transport is fundamental to the role occupied by the gastrointestinal (GI) tract in oxalate homeostasis. The absorption of dietary oxalate, together with its secretion into the intestine, and degradation by the gut microbiota, can all influence the excretion of this nonfunctional terminal metabolite in the urine. Knowledge of the transport mechanisms is relevant to understanding the pathophysiology of hyperoxaluria, a risk factor in kidney stone formation, for which the intestine also offers a potential means of treatment. The following discussion presents an expansive review of intestinal oxalate transport. We begin with an overview of the fate of oxalate, focusing on the sources, rates, and locations of absorption and secretion along the GI tract. We then consider the mechanisms and pathways of transport across the epithelial barrier, discussing the transcellular, and paracellular components. There is an emphasis on the membrane-bound anion transporters, in particular, those belonging to the large multifunctional Slc26 gene family, many of which are expressed throughout the GI tract, and we summarize what is currently known about their participation in oxalate transport. In the final section, we examine the physiological stimuli proposed to be involved in regulating some of these pathways, encompassing intestinal adaptations in response to chronic kidney disease, metabolic acid-base disorders, obesity, and following gastric bypass surgery. There is also an update on research into the probiotic, Oxalobacter formigenes, and the basis of its unique interaction with the gut epithelium.

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The anion exchanger PAT-1 (Slc26a6) does not participate in oxalate or chloride transport by mouse large intestine

Cited by 7 publications

References 92 publications

The role of NHE3 (Slc9a3) in oxalate and sodium transport by mouse intestine and regulation by cAMP

The role of NHE3 (Slc9a3) in oxalate and sodium transport by mouse intestine and regulation by cAMP

Oxalate as a potent promoter of kidney stone formation

Oxalate Flux Across the Intestine: Contributions from Membrane Transporters

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