The anomaly of the $\nu$<sub>1</sub>‐resonance Raman band of bβ‐carotene in solution and in photosystem I and II

Tschirner, Norman; Brose, Katharina; Schenderlein, Matthias; Zouni, Athina; Schlodder, E.; Mroginski, María Andrea; Hildebrandt, Peter; Thomsen, C.

doi:10.1002/pssb.200982299

Cited by 19 publications

(10 citation statements)

References 19 publications

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“…Vibrational mode Assignment 747 γ(C-O-H) of COOH Pectin (Synytsya et al, 2003) 915 ν(C-O-C) in plane, symmetric Cellulose, lignin (Edwards et al, 1997) 1000 ν 3 (C-CH 3 stretching) and phenylalanine Carotenoids (Tschirner et al, 2009;Kurouski et al, 2015) 1048-1068…”

Section: Bandmentioning

confidence: 99%

Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Salinity Stresses, Nitrogen, Phosphorus, and Potassium Deficiencies in Rice

et al. 2020

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Proper management of nutrients in agricultural systems is critically important for maximizing crop yields while simultaneously minimizing the health and environmental impacts of pollution from fertilizers. These goals can be achieved by timely confirmatory diagnostics of nutrient deficiencies in plants, which enable precise administration of fertilizers and other supplementation in fields. Traditionally, nutrient diagnostics are performed by wet-laboratory analyses, which are both time-and labor-consuming. Unmanned aerial vehicle (UAV) and satellite imaging have offered a non-invasive alternative. However, these imaging approaches do not have sufficient specificity, and they are only capable of detecting symptomatic stages of nutrient deficiencies. Raman spectroscopy (RS) is a non-invasive and non-destructive technique that can be used for confirmatory detection and identification of both biotic and abiotic stresses on plants. Herein, we show the use of a hand-held Raman spectrometer for highly accurate presymptomatic diagnostics of nitrogen, phosphorus, and potassium deficiencies in rice (Oryza sativa). Moreover, we demonstrate that RS can also be used for pre symptomatic diagnostics of medium and high salinity stresses. A Raman-based analysis is fast (1 s required for spectral acquisition), portable (measurements can be taken directly in the field), and label-free (no chemicals are needed). These advantages will allow RS to transform agricultural practices, enabling precision agriculture in the near future.

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Section: Bandmentioning

confidence: 99%

Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Salinity Stresses, Nitrogen, Phosphorus, and Potassium Deficiencies in Rice

et al. 2020

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“…Vibrational mode Assignment 747 γ(C─O─H) of COOH Pectin [25] 915 ν(C─O─C) in plane, symmetric Cellulose and lignin [26] 1,000 ν 3 (C─CH 3 stretching) and phenylalanine Carotenoids and protein [27,28] 1,155 asym ν(C─C) ring breathing Carbohydrates and cellulose [26] 1,184 ν(C─O─H) next to aromatic ring+σ (CH) Xylan [29,30] 1,218-1,226 δ(C─C─H) Aliphatic [31] and xylan [29] 1,247 C─O stretching (aromatic) Lignin [32] 1,288 δ(C─C─H) Aliphatic [31] 1,326 δCH 2 bending vibration Cellulose and lignin [26] 1,382 δCH 2 bending vibration Aliphatic [31] 1,440 δ (CH 2 ) + δ (CH 3 ) Aliphatic [31] 1,455 δCH 2 bending vibration Aliphatic [31] 1,488 δ (CH 2 ) + δ (CH 3 ) Aliphatic [31] 1,527-1,551 ─C═C─ (in plane) Carotenoids [33,34] 1,601 ν(C─C) aromatic ring+σ (CH) Lignin [35,36] 1,630 C═C─C (ring) Lignin [35][36][37] intensities of all other bands in the spectra collected from leaves of CA and HLB + BL exhibited only very small variations compared with the spectra collected from leaves of HL trees.…”

Section: Bandmentioning

confidence: 99%

Detection and identification of canker and blight on orange trees using a hand‐held Raman spectrometer

Sanchez

Pant²,

Irey³

et al. 2019

J Raman Spectroscopy

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Huanglongbing (HLB), or citrus greening, is a devastating disease of citrus that is debilitating the U.S. citrus industry. The infected trees exhibit yellowing leaves, premature defoliation, and ultimately death of the entire plant. In addition to the devastating impact of HLB alone, infected trees become an easy target for other diseases. This further decreases the fruit yield, shortens the tree life, and complicates management practices. Raman spectroscopy is a noninvasive and nondestructive analytical technique that provides insight on the chemical structure of a specimen. In this study, we demonstrate that utilization of a hand‐held Raman spectrometer in combination with chemometric analyses enables detection and identification of the secondary disease such as blight on HLB‐infected orange trees (HLB + BL). We also showed that using this spectroscopic approach, we could detect and identify canker and distinguish this disease from healthy trees, HLB, and HLB + BL with high accuracy.

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“…30 Besides, Tschirner et al 28 found a wavelength-dependent variation of the peak maximum of the C=C stretch vibration for β-carotene in dichloromethane and photosystems I and II 28 that they attributed to different relative resonances of the two underlying modes, and pointed out that their quantum chemical calculations could not reproduce the effect. 28,31 According to their experimental data, the peak maximum shifts from 1525 cm −1 at 1064 nm to 1521 cm −1 at around 514 nm and up again to Raman spectra are mapped for excitation wavelengths between 446 nm and 534 nm, and the resonance profile of the strongest carotenoid Raman peak at ca. 1528 cm −1 (C=C stretch) is displayed here.…”

Section: Raman Peak Positionsmentioning

confidence: 99%

Absorption and resonance Raman characteristics of β-carotene in water-ethanol mixtures, emulsion and hydrogel

et al. 2018

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Absorption or resonance Raman scattering are often used to identify and even quantify carotenoids in situ. We studied the absorption spectra, the Raman spectra and their resonance behavior of β-carotene in different molecular environments set up as mixtures from lipid (emulsion) and non-polar (ethanol) solvents and a polar component (water) with regard to their application as references for in situ measurement. We show how both absorption profiles and resonance spectra of β-carotene strongly depend on the molecular environment. Most notably, our data suggests that the characteristic bathochromic absorption peak of J-aggregates does not contribute to carotenoid resonance conditions, and show how the Raman shift of the C=C stretching mode is dependent on both, the molecular environment and the excitation wavelength. Overall, the spectroscopic data collected here is highly relevant for the interpretation of in situ spectroscopic data in terms of carotenoid identification and quantification by resonance Raman spectroscopy as well as the preparation of reference samples. In particular, our data promotes careful consideration of appropriate molecular environment for reference samples.

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The anomaly of the $\nu$₁‐resonance Raman band of bβ‐carotene in solution and in photosystem I and II

Cited by 19 publications

References 19 publications

Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Salinity Stresses, Nitrogen, Phosphorus, and Potassium Deficiencies in Rice

Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Salinity Stresses, Nitrogen, Phosphorus, and Potassium Deficiencies in Rice

Detection and identification of canker and blight on orange trees using a hand‐held Raman spectrometer

Absorption and resonance Raman characteristics of β-carotene in water-ethanol mixtures, emulsion and hydrogel

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The anomaly of the $\nu$1‐resonance Raman band of bβ‐carotene in solution and in photosystem I and II

Cited by 19 publications

References 19 publications

Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Salinity Stresses, Nitrogen, Phosphorus, and Potassium Deficiencies in Rice

Raman Spectroscopy Enables Non-invasive and Confirmatory Diagnostics of Salinity Stresses, Nitrogen, Phosphorus, and Potassium Deficiencies in Rice

Detection and identification of canker and blight on orange trees using a hand‐held Raman spectrometer

Absorption and resonance Raman characteristics of β-carotene in water-ethanol mixtures, emulsion and hydrogel

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The anomaly of the $\nu$₁‐resonance Raman band of bβ‐carotene in solution and in photosystem I and II