The anti-anemic effect of dried beet green in phenylhydrazine treated rats

Abstract

Phenylhydrazine (PHZ) and its derivatives were used firstly as antipyretics but the toxic action on red blood cells made their use dangerous. The aim of this study was to estimate the contents of iron and vitamins in different parts of beetroot as root and beet green (leave and stalk together) as well as their ethanol extracts. Then, the biochemical evaluation for the highest iron and vitamins contents as an anti-anemic effect against anemic rats caused by phenylhydrazine were studied. Anemia induction caused by intraperitoneal injection of phenylhydrazine at 40 mg/kg for 2 days. Rats were randomly divided into four groups (n=10/group). Group, I fed a standard diet (normal control). Group II fed standard diet contained 5% dried beet green for normal rats. Group III fed a standard diet for anemic rats. Group IV fed a standard diet containing 5% dried beet green for anemic rats. It was assayed the complete blood count (CBC): red blood cell (RBC) count, hemoglobin concentration (Hb) and haematocrit percentage (HCT) as indices measured of anaemia as well as white blood cell (WBC) count at the third day (D3), twenty-one day (D21) and forty-two day (D42). Also, the activity of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), the levels of serum urea, uric acid, creatinine and malondialdehyde (MDA), plasma hydrogen peroxide (H2O2) and total antioxidants capacity (TAC) contents were analyzed according to manufacturer's instructions. The dried beet green is a rich source of iron and vitamins contents. After feeding on standard diet containing 5% dried beet green for anemic rats, showed that levels of RBC, HB and HCT were increased and the WBC count was improved compared to anemic control group. The activity of ALT, AST, and ALP, the levels of serum urea, creatinine and MDA and plasma H2O2 content were significantly ameliorated while the TAC was significantly increased compared to the anemic control group. In addition, the results of the histological examination coincided with the results of blood analysis. We conclude that nutrition of 5% dried beet green for forty-two days is safe, non-toxic and reduces anemia.

Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats

“…On the other hand, the study showed after 20 days taking 8 g of beetroot the level of total iron binding capacity decreased and serum iron levels increased. This indicates that beetroot is an excellent source of iron [27]. 1a, b, c, d, e, significantly different from negative or positive control at p<0.05.…”

“…Also, vitamin B6 and B12 were in values of 5.46 and 54.48 mg /100g in beetroot compared to 0.081 and 0.00 mg /100g in eggplant, respectively as seen in table 1. Dried beet green has been a rich source of vitamins B6, B12, and folic acid [27]. The chemical composition of this part was estimated to prepare a diet to reduce anemia caused by PHZ in rats, anti-anemic effect of dried beet green in phenylhydrazine treated rats was approved Elaby and Ali., 2018 [27].…”

“…Dried beet green has been a rich source of vitamins B6, B12, and folic acid [27]. The chemical composition of this part was estimated to prepare a diet to reduce anemia caused by PHZ in rats, anti-anemic effect of dried beet green in phenylhydrazine treated rats was approved Elaby and Ali., 2018 [27]. On the other hand, the mean value of folate in beetroot and eggplant were 9.70 and0.02mg/100g, respectively.…”

“…The values of WBCs in all tested groups were returned back compared to negative control value with non-significant different among them as seen in figure 2A. The result was in agreement with Elaby and Ali., 2018 [27]. Con -: Rats fed on basal diet.…”

Antianemic activity of polyherbal formulation, Raktavardhak Kadha, against phenylhydrazine-induced anemia in rats

“…The phenylhydrazine-induced erythropenia lowered Hb and reduced HCT levels which persisted for 8–12 days [ 13 ]. Furthermore, phenylhydrazine also induces pathological changes in tissues such as heart, kidney, liver and spleen [ 16 ]. Therefore to screen the antianemic potential of RK, we evaluated levels of RBCs, Hb and HCT during experimental period as well as histopathological studies of major organs at the end of experimental period.…”