The Anti-Angiogenic Activity of a Cystatin F Homologue from the Buccal Glands of Lampetra morii

Zhu, Mingru; Li, Bowen; Wang, Jihong; Xiao, Rong

doi:10.3390/md16120477

Cited by 5 publications

(2 citation statements)

References 45 publications

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“…Amblyomin-X, a serine protease inhibitor from Amblyomma cajennense tick, impaired VEGF-A-induced angiogenesis by interfering with platelet-endothelial cell adhesion molecule-1 (PECAM-1) expression (31). Furthermore, a cystatin F homolog obtained from the buccal glands of Lampetra morii, which can suppress the activity of C1 cysteine proteases, was revealed to inhibit the key steps of angiogenesis, such as proliferation, adhesion, migration, invasion, and tube formation of human umbilical vein endothelial cells (HUVECs) (32). However, the antiangiogenic activities and underlying molecular mechanisms of ahpatinins C, E, and G have not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Antiangiogenic potentials of ahpatinins obtained from a Streptomyces species

Han

Jang

et al. 2019

Oncol Rep

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While exploring new angiogenesis inhibitors from microbial metabolites, we recently isolated ahpatinins C, E, and G from a soil-derived Streptomyces sp. 15JA150. Ahpatinins C, E and G are known to have pepsin and renin inhibitory activities; however, their antiangiogenic activities and underlying molecular mechanisms have not been fully elucidated. In the present study, the antiangiogenic properties of ahpatinins C, E and G were investigated. The results revealed that the natural compounds significantly inhibited the vascular endothelial growth factor (VEGF)-induced proliferation, invasion, adhesion, and tube formation of human umbilical vein endothelial cells (HUVECs) without exhibiting any cytotoxicity. It was also revealed that ahpatinin E effectively suppressed the neovascularization of the chorioallantoic membranes in growing chick embryos. Notably, ahpatinins C, E, and G led to the downregulation of VEGF-induced activation of VEGF receptor 2 (VEGFR2) and its downstream signaling mediators, including AKT, ERK1/2, JNK, p38, and NF-κB, in HUVECs. Moreover, they reduced the expression of matrix metalloproteinase (MMP)-2 and MMP-9 in the HUVECs following stimulation with VEGF. Furthermore, ahpatinins C, E, and G reduced the tumor cell-induced invasion and tube forming abilities of HUVECs, as well as the expression of VEGF, by suppressing hypoxia-inducible factor-1α (HIF-1α) activity in U87MG glioblastoma cells. Collectively, the present findings indicated that ahpatinins C, E, and G may be used in anticancer therapy by targeting tumor angiogenesis through the inhibition of both VEGFR2 and HIF-1α pathways.

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Section: Introductionmentioning

confidence: 99%

Antiangiogenic potentials of ahpatinins obtained from a Streptomyces species

Han

Jang

et al. 2019

Oncol Rep

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show abstract

“…Zhu and colleagues showed the antiangiogenic activity of rLm-cystatin F , a homologue of cystatin F, which was isolated from the buccal glands of Lampetra morii . rLm-cystatin F can suppress the migration, invasion, adhesion, and tube formation of HUVEC cells [ 26 ]. Ting and Chen demonstrated the anticancer activity of the antimicrobial peptide tilapia piscidin 4 (TP4) derived from the fish species Nile tilapia in non-small cell lung cancer cells.…”

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confidence: 99%