The Anti-depressive Effect of Samul-tanggahyangbuja on Chronic Mild Stress in Ovariectomized Rats

Abstract

Objectives: The purpose of the present study is to investigate anti-depressive effects of Samul-tanggahyangbuja (SGH) on ovariectomized and chronic mild stress (CMS) induced rats. Methods: Ovariectomized rats were exposed to CMS for 4 weeks. Changes of depression behavior were tested by using sucrose intake test (SIT), elevated plus maze (EPM), forced swimming test (FST) and Morris water maze test (MWMT) in rats until being orally medicated with SGH (100 or 400 mg/kg/day). In addition, the serum levels of corticosterone (CORT), IL-4, IL-1β and changes of 5-HT in the brain were measured. Results: 1. SGH 400 mg/kg treated group (SGH 400) significantly increased amount of sucrose intake compared with the control group (p<0.05). 2. SGH 100 mg/kg treated group (SGH 100) and SGH 400 significantly increased the time spent in the open arms of the EPM compared with the control group (p<0.01). SGH 400 also significantly increased the number of crossing of the open and closed arms compared with the control group (p<0.05). 3. SGH significantly shortened the immobility time in FST compared with the control group (SGH 100 p<0.05, SGH 400 p<0.01). 4. SGH significantly increased performance of acquisition trials compared with the control group (p<0.05, on day 4, 5 of SGH 100 and 400). SGH 400 also significantly increased performance of retention trials compared with the control group (p<0.05). 5. The serum levels of corticosterone and IL-4 were not significantly different among the groups. There were no changes on the serum levels of corticosterone, IL-1β and IL-4 after administration with SGH. 6. SGH 400 significantly increased the level of 5-HT in the hippocampus compared with the control group (p<0.05). SGH significantly increased the levels of 5-HT in the hypothalamus compared with the control group (SGH 100 p<0.05, SGH 400 p<0.01). Conclusions: These results suggest that SGH has the anti-depressive effect on ovariectomized rat and affect 5-HT system rather than hypothalamic-pituitary-adrenal (HPA) axis and immune system.

Prescription patterns of herbal medicine for polycystic ovarian syndrome in major Korean medicine hospitals: a multicenter retrospective study

“…A study evaluating the effectiveness of an herbal-based obesity management program on an obese PCOS patient concluded that Changbudodam-tang (Cangfu Daotan-tang) is effective in treating oligomenorrhea associated with obesity in PCOS [20]. Additionally, herbal prescriptions such as Chokyung-san (Tiaojing-san), Changbudodamtang (Cangfu Daotan-tang), Onkyung-tang (Wenjingtang), Gyejibokryeong-hwan (Guizhi Fuling-wan), and Chokyungjongok-tang (Tiaojing Zhongyu-tang) were found to alleviate ovulatory dysfunction and apoptosis of granulosa cells, normalize the endometrium, and improve acne vulgaris, oligomenorrhea and excess androgen-related conditions associated with female ovarian dysfunction [21][22][23]. Several studies using animal PCOS models also tested the effectiveness of major herbal medicine prescriptions, such as Chokyung-san (Tiaojing-san), Gamiguibi-tang (Jiawei Guipi-tang), and Changbudodam-tang (Cangfu Daotan-tang) [23][24][25][26][27], and presented favorable results.…”

The Effect of Poria Cocos Pharmacopuncture at SP6(Sameumgyo) and ST36(Joksamni) on Chronic Stress Model(CSM) Rats

“…모델 흰쥐에 대하여 四物湯加香附子의 항우울 효과 9) , 加 減酸棗仁湯의 항스트레스 효과 10) , 하엽추출물의 항우울 효과 11) 등이 있고, 白茯笭藥鍼에 대한 실험적 연구로 문 등이 정상 흰쥐의 五臟兪穴에 白茯笭藥鍼을 투여하여 腎 臟機能에 유의한 효과를 얻은 실험 12) , Kim et al이 흰쥐 의 陰谷穴에 白茯笭藥鍼을 투여하여 Lipopolysaccharide 로 유발된 신기능 손상에 유효한 효과 13…”

“…足三里와 三陰交는 寧心健脾, 鎭靜安神하는 효능이 있어 서 정신신경계 질환에 다빈도 처방이다 36) . 지금까지의 스트레스에 대한 연구는 실험방법이 약물 의 경구투여 방식 [9][10][11]…”