The anti-hepatoma effect of nanosized Mn–Zn ferrite magnetic fluid hyperthermia associated with radiationin vitroandin vivo

Abstract

Joint therapy is a promising area of study in cancer treatment. In this paper, we prepared Mn-Zn ferrite (Mn0.5Zn0.5Fe2O4) magnetofluid using PEI as a surfactant, and investigated the anticancer effect of Mn0.5Zn0.5Fe2O4 magnetic fluid hyperthermia (MFH) combined with radiotherapy on hepatocellular carcinoma. Both in vitro and in vivo results suggest that this combined treatment with MFH and radiation has a better therapeutic effect than either of them alone. The apoptotic rate and necrotic rate of the combined treatment group was 38.80 and 25.20%, respectively. In contrast, it was only 7.49 and 3.62% in the radiation-alone group, 15.23 and 7.90% in the MFH-alone group, only 3.52 and 2.16% in the blank control group, and 23.56 and 27.56% in the adriamycin group. The cell proliferation inhibition rate of the combined treatment group (88.5%) was significantly higher than that of the radiation-alone group (37.5%), MFH-alone group (60.6%) and adriamycin group (70.6%). The tumor volume inhibition and mass inhibition rate of the combined treatment group was 87.62 and 88.62%, respectively, obviously higher than the 41.04 and 34.20% of the radiation-alone group, 79.87 and 77.92% of the MFH-alone group and 71.76 and 66.87% of the adriamycin group. It is therefore concluded that this combined application of MFH and radiation can give good synergistic and complementary effects, which offers a viable approach for treatment of cancer.

Magnetic nanoparticle clusters radiosensitise human nasopharyngeal and lung cancer cells after alternating magnetic field treatment

“…In clinical trials, Klaus et al used magnetic hyperthermia combined with radiotherapy to treat glioblastoma multiforme patients, and found that the combination therapy more effectively shrank tumours than either one of the therapies alone [45]. Using flow cytometry, we found that MNCs under an AMF displayed a slightly better killing effect than radiotherapy alone (at a dose of 4 Gy), while the group exposed to a combination of both treatments showed the highest apoptosis rate, which is consistent with the results reported by Lin et al [46]. The SER for CNE-2 and NCI-H460 cells was 1.35 and 1.44, respectively, confirming their radiosensitisation effect.…”

Magnetic nanoparticle-based therapeutic agents for thermo-chemotherapy treatment of cancer

“…122 They chose a magnetic fluid with a NP concentration of 10 g/L because it stabilized around 43 °C under application of an AMF of frequency = 230 kHz and amplitude = 1 kA/m. Tumours were grown into the right posterior limb rump of nude mice by injecting exponentially growing HepG2 cells.…”

Core/Shell Nanoparticles of Non-Stoichiometric Zn–Mn and Zn–Co Ferrites as Thermosensitive Heat Sources for Magnetic Fluid Hyperthermia

“…It therefore leads to increasing thermomagnetic coefficient 49,50 (݇ ்ெ = −∆ߪ ∆ܶ ⁄ , where ߪ is the magnetization per unit mass) and decreasing Curie temperature hence limiting the upper temperature reached by MFH to the safety range (41 -45 o C) [49][50][51][52][53][54] . This characteristic would be very promising because it could enable a local self-regulated temperature control 55,56 , which would avoid damages to healthy tissues due to overheating.…”