The Anti-inflammatory Action of a Xanthine Oxidase Inhibitor (Allopurinol)

The Antinociceptive and Anti-Exudative Action of a Xanthine Oxidase Inhibitor (Allopurinol) and of Xanthine

“…It is evident from the data presented in this paper, as well as from those published in a previous communication [4], that allopurinol is ca pable of inhibiting various models of increased vascular permeability, such as the mouse peritonitis models employed in this study as well as the early phases of turpentine-induced pleurisy and kaolin-induced paw oedema in the rat. The dosage range in which allopurinol exerts a significant anti exudative activity in the mouse peritonitis (3-30 mg/kg s.c., 30-100 mg/kg p.o.)…”

“…The dosage range in which allopurinol exerts a significant anti exudative activity in the mouse peritonitis (3-30 mg/kg s.c., 30-100 mg/kg p.o.) is much the same as that at which the compound was previously found to inhibit pleurisy or paw oedema in the rat [4], In addition to be ing anti-inflammatory in the above-mentioned models of an acute inflam matory reaction in two rodent species, allopurinol has now been shown to possess 'mild analgesic' properties. Taken together these effects further corroborate the hypothesis put forward in a previous paper [4] that allo purinol may well owe part of its clinical usefulness in gout not only to its inhibitory effect on xanthine oxidase.…”

“…In a recent report from this laboratory it was shown that 4-hydroxypyrazolo [3,4-d]pyrimidine (allopurinol) is capable of reducing predomi nantly the exudative phase-component of inflammatory reactions such as turpentine-induced pleurisy and kaolin-elicited paw oedema in the rat [4]. It was concluded 'that the compound might be beneficial in the case of acute attacks (of gout) in which "early" inflammatory changes possibly elicited by kinin-like mediators would seem to prevail'.…”

The Euglobulin Lysis Time: A Method for Evaluating the Plasma Fibrinolytic Activity of Normal and Arthritic Rats and the Therapeutic Value of Antiinflammatory (Steroidal and Non-steroidal) Agents

“…On days 18 and 22 post-adjuvant, the intensity of the secondary lesions of the right (uninjected) hind-paw was determined volumetrically and compared with that of control arthritic rats receiving saline instead of a drug. Body-weight changes were likewise recorded as a means for assessing the intensity of the disease [21]. …”

“…The citrated blood was centrifuged at 700 x g for 20 min at 0°C, the plasma thus obtained immediately frozen and stored at -18°C until used. The euglo bulin fraction was obtained according to Wintrobe [21] by precipitation with 0.1 N HC1 at pH 5.3 and centrifugation at 700 x g for 4 min at 0°C. The precip itate was dissolved in Veronal acetate buffer diluted with physiological saline (1 : 4; pH 7.42).…”