The anti-inflammatory and osteogenic activity of chitosan/polyvinyl alcohol/graphene oxide/astaxanthin nanofibers membranes in vitro study

Abstract

Anti-inflammation and bone regeneration are the two major goals of periodontal therapy. We have demonstrated that chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO)/astaxanthin (ASTA) nanofibers membranes prepared by electrospinning had favorable micro-morphology, good mechanical properties, and no cytotoxicity. In this study, CS/PVA/GO/ASTA nanofibers membranes were prepared to modulate both inflammatory response and osteogenic induction in vitro study. When the nanofibers membranes were co-cultured with RAW264.7 cells, glycoprotein nonmetastatic melanoma protein in the cells was highly expressed and RAW264.7 cells were polarized to M2 phenotype at the same time. In addition, following stimulation with nanofibers membranes, the messenger RNA (mRNA) and protein levels of Osteocalcin (OCN) and Runx2 in Bone marrow mesenchymal stem cells (BMSCs) were highly expressed. Taken together, these results suggested CS/PVA/GO/ASTA nanofibers membranes may promote the dissipation of inflammation and stimulate the differentiation of BMSCs into osteoblasts.

Synthesis and characterization of gentamicin loaded ZSM-5 scaffold: Cytocompatibility and antibacterial activity

“…It can be related to the negative effect of antibiotics on cellular activities. [43][44][45][46][47][48][49][50][51][52][53][54][55][56] These results are also confirmed by quantitative results from sample absorbance at 612 nm (Figure 7(b)). Kumar et al 57 reported a similar result by increasing the concentration of GEN on calcium deposition.…”

Polyvinyl Alcohol-Chitosan Scaffold for Tissue Engineering and Regenerative Medicine Application: A Review

“…The electrospun membranes were found to stimulate a significant upregulation of osteogenic genes, osteocalcin (OCN), and RUNX2 in BMMSCs, and high expression of GPNMB in RAW26407 cells, leading to M2 polarization. These findings suggest that the nanofiber membranes can potentially enhance inflammatory dissipation and osteoblast differentiation [ 95 ].…”