The anti-invasive activity of Robinia pseudoacacia L. and Amorpha fruticosa L. on breast cancer MDA-MB-231 cell line

Cvetković, Danijela; Jovankić, Jovana; Milutinović, Milena; Nikodijević, Danijela D.; Grbović, Filip; Ćirić, Andrija; Topuzović, Marina; Marković, Snežana D.

doi:10.2478/s11756-019-00257-4

Cited by 4 publications

(3 citation statements)

References 57 publications

(54 reference statements)

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“…That extract did not express the cytotoxic effect against the palatal mesenchymal stem cells. Cvetković et al [ 41 ] analyzed the cytotoxic activity of methanolic extract of black locust flower obtained using Soxhlet extraction. They concluded that the extract had no significant cytotoxic activity on MRC-5 and MDA-MB-231 (human breast cancer) cell lines.…”

Section: Resultsmentioning

confidence: 99%

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Boškov,

Savić,

Grozdanić Stanisavljević

et al. 2024

Polymers

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Black locust flower extract contains various polyphenols and their glucosides contribute to the potential health benefits. After intake of these bioactive compounds and passage through the gastrointestinal tract, their degradation can occur and lead to a loss of biological activity. To overcome this problem, the bioactive compounds should be protected from environmental conditions. This study aimed to encapsulate the black flower extract in the microparticles based on biodegradable polysaccharides, alginate, and chitosan. In the extract, the total antioxidant content was found to be 3.18 ± 0.01 g gallic acid equivalent per 100 g of dry weight. Also, the presence of lipids (16), phenolics (27), organic acids (4), L-aspartic acid derivative, questinol, gibberellic acid, sterol, and saponins (2) was confirmed using the UHPLC–ESI–MS analysis. In vitro assays showed that the extract has weak anti-α-glucosidase activity and moderate antioxidant and cytotoxic activity against the HeLa cell line. The extrusion method with secondary air flow enabled the preparation of microparticles (about 270 μm) encapsulated with extract. An encapsulation efficiency of over 92% was achieved in the alginate and alginate–chitosan microparticles. The swelling study confirmed a lower permeability of alginate–chitosan microparticles compared with alginate microparticles. For both types of microparticles, the release profile of antioxidants in the simulated gastrointestinal fluids at 37 °C followed the Korsmeyer–Peppas model. A lower diffusion coefficient than 0.5 indicated the simple Fick diffusion of antioxidants. The alginate–chitosan microparticles enabled a more sustained release of antioxidants from extract compared to the alginate microparticles. The obtained results indicated an improvement in the antioxidant activity of bioactive compounds from the extract and their protection from degradation in the simulated gastric conditions via encapsulation in the polymer matrixes. Alginate–chitosan showed slightly slower cumulative antioxidant release from microparticles and better antioxidant activity of the extract compared to the alginate system. According to these results, alginate–chitosan microparticles are more suitable for further application in the encapsulation of black locust flower extract. Also, the proposed polymer matrix as a drug delivery system is safe for human use due to its biodegradability and non-toxicity.

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Section: Resultsmentioning

confidence: 99%

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Boškov,

Savić,

Grozdanić Stanisavljević

et al. 2024

Polymers

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“…Results showed that all traits were influenced by accessions, a key that may also demonstrate genetic content that probably influences variation. Furthermore, studies have been conducted on the expression of genes associated with drought [25], phenolic constituents [26], proteomic analysis of symbiotic proteins [27], and cancer research [28,29]. The aforementioned research signifies from one point of view the significance of A. fruticosa, although we cannot eliminate the negative effects that it has on ecosystems due to its rapid expansion.…”

Section: Discussionmentioning

confidence: 99%

First Report and Genetic Analysis of the Invasive Species A. fruticosa L. in Greece: A Combined Genetic and Regeneration Study

Avramidou,

Korakaki,

Malliarou

et al. 2023

Ecologies

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A first assessment of genetic diversity and expansion of the invasive species Amorpha fruticosa L. was estimated in the 91E0* (Annex I Dir. 92/43/EEC) priority habitat of Nestos. A. fruticosa is a perennial deciduous shrub belonging to the family Fabaceae and is native to the south-western part of North America, which recently was introduced to Europe as an ornamental plant. Its expansion through the cross-border river of Nestos has significant negative effects on the 91E0* habitat (a habitat with Alnus glutinosa, Populus alba, and Ulmus minor). Herein, we studied the genetic diversity with amplified fragment length polymorphisms fragments of eastern and western populations in order to see if there is one genetic pool for the species. Furthermore, we estimated the regeneration index (IR) in order to see the potential expansion of this invasive species. Results showed that eastern and western populations have almost equal heterozygosity (He) and with a slightly higher value for the western population, which was in agreement with the results of the IR. This is an innovative study that discusses the implications of A. fruticosa of 91E0* habitat in Nestos and proposes management measures to eliminate its expansion and improve the resilience of the habitat.

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“…In modern medicine, application have been diversified mainly due to beneficial properties of rotenoids and isoflavones. Therefore, A. fruticosa is used for stimulating immunity, treating diabetes, metabolic disease, and cancer (Cvetković et al, 2019 ; Kozuharova et al, 2017 ; Lee et al, 2006 ; Lee et al, 2016 ), as well as possessing antimicrobial properties and for treating stomach pain, intestinal worms, eczema, neuralgia, carbuncle, burns, wounds, and rheumatism. In search for possible antitumor agents, Li et al ( 1993 ) extracted 8 novel cytotoxic compounds, belonging to rotenoids and isoflavones.…”

Section: Future Prospects In Management Of a Fruticosamentioning

confidence: 99%

A review on invasive false indigo bush (Amorpha fruticosaL.): Nuisance plant with multiple benefits

Grabić

Ljevnaić‐Mašić

Zhan

et al. 2022

Ecology and Evolution

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Increased mobility of people around the globe has facilitated transferring species to new environments, where some have found suitable conditions and even become invasive. False indigo‐bush (Amorpha fruticosa L.) is a plant native to North America but has intentionally or unintentionally spread over the Northern Hemisphere, where it often becomes invasive. The plant is especially easily dispersed within the watersheds of large rivers, where seasonal flooding is regular. Seeds and other propagules are buoyant, and when the water recedes, new plants emerge, forming dense thickets where only a few other species can co‐exist. In order to sustain native biodiversity, spread control is needed. However, mechanical control and eradication measures currently in use are labor demanding and costly, while application of herbicides is limited. On the other hand, the plant possesses a number of beneficial properties, such as phytochemical applications (medical and insecticidal effects), biocoenotic uses (honey plant, ornamental features), and ecosystem services (soil stabilization, provision of food for animals, and fiber and biomass for industry, e.g., nanocellulose). For the reasons above mentioned, the plant is considered quite controversial, and the paper discusses both aspects: potential detrimental effects when introduced to new habitats and its beneficial uses for human society. In addition, the paper presents alternative measures of spreading control (e.g., grazing) and argues that exploiting it for beneficial purposes might help spread control, thus covering the expenses of controlling its distribution.

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The anti-invasive activity of Robinia pseudoacacia L. and Amorpha fruticosa L. on breast cancer MDA-MB-231 cell line

Cited by 4 publications

References 57 publications

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

First Report and Genetic Analysis of the Invasive Species A. fruticosa L. in Greece: A Combined Genetic and Regeneration Study

A review on invasive false indigo bush (Amorpha fruticosaL.): Nuisance plant with multiple benefits

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