The Anti-<i>Helicobacter</i> <i>pylori</i> Effects <i>of Lactobacillus acidophilus</i>, <i>L. plantarum</i>, and <i>L. rhamnosus</i> in Stomach Tissue of C57BL/6 Mice

Abstract

&lt;b&gt;&lt;i&gt;Background and Aim:&lt;/i&gt;&lt;/b&gt; &lt;i&gt;Helicobacter pylori&lt;/i&gt; is one of the most common pathogenic bacteria in the human gut, and is also one of the most important factors that cause digestive disorders such as chronic inflammation, gastric ulcers, and even gastric cancer. Since the use of various antibiotics to treat &lt;i&gt;H. pylori&lt;/i&gt; infection is associated with the development of resistance in this bacterium, the aim of this study was to determine the anti-&lt;i&gt;H. pylori&lt;/i&gt; effects of &lt;i&gt;Lactobacillus acidophilus&lt;/i&gt;, &lt;i&gt;L. plantarum&lt;/i&gt;, and &lt;i&gt;L. rhamnosus&lt;/i&gt; in the stomach tissue of C57BL/6 mice. &lt;b&gt;&lt;i&gt;Materials and Methods:&lt;/i&gt;&lt;/b&gt; In this experimental study, 70 mice in ten groups were evaluated from July to September 2017 in the microbiology laboratory of the School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. After induction of &lt;i&gt;H. pylori&lt;/i&gt; infection in mice with the standard strain of &lt;i&gt;H. pylori&lt;/i&gt; (ATCC 43504), the infected mice were treated with drug and &lt;i&gt;Lactobacillus&lt;/i&gt; species in different groups. Then, the anti-&lt;i&gt;H. pylori&lt;/i&gt; effects of lactobacilli were evaluated by stool antigen test and tissue staining. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; Based on ELISA results and histological findings, a reduction of inflammation was observed. The group which was only exposed to &lt;i&gt;L. rhamnosus&lt;/i&gt; and the one which was exposed to all three strains of &lt;i&gt;Lactobacillus&lt;/i&gt; showed the highest antimicrobial effect on &lt;i&gt;H. pylori&lt;/i&gt;. &lt;b&gt;&lt;i&gt;Conclusion:&lt;/i&gt;&lt;/b&gt; According to the results of this study, probiotic bacteria including &lt;i&gt;L. acidophilus&lt;/i&gt;, &lt;i&gt;L. plantarum&lt;/i&gt;, and &lt;i&gt;L. rhamnosus&lt;/i&gt; could be useful in the reduction of &lt;i&gt;H. pylori&lt;/i&gt; infection in the mouse model.

Association of LRRK2 rs11564258 single nucleotide polymorphisms with type and extent of gastrointestinal mycobiome in ulcerative colitis: a case–control study

“…We also reported Pichia kudriavzevii, a specific species that was not detected in previous IBD studies. These intestinal fungi alterations observed in UC patients might also lead to the development of specific fungal probiotics that lessens the inflammatory status resembling bacterial probiotics [ 31 , 32 ].…”

Eradication therapy of helicobacteriosis with probiotics, problems, and prospects

“…Как и в случае изучения эффектов пробиотиков в сиcтеме in vitro при использовании биологических моделей были обнаружены специфические особенности пробиотических штаммов. Это касалось не только антихеликобактерной активностью, отмеченной в большинстве статей, но и в ингибированием адгезии и инвазии [36], снижением лейкоцитарной инфильтрации, уменьшением продукции провоспалительных цитокинов, в частности, IL-8 [57,58,60], подавлением экспрессии генов патогенности НР [36].…”

Uso De Lactobacillus Na Erradicação De Helicobacter Pylori E Na Redução De Reações Adversas Ao Tratamento Convencional: Uma Revisão Integrativa

“…Em diversos estudos 15,19,21,30 , os resultados foram promissores em relação ao uso de Lactobacilos contra a infecção por H. pylori, juntamente com o tratamento de erradicação. E ainda há como, ponto positivo, para o uso de cepas de Lactobacilos, a redução das reações causados pela terapia com antibióticos e bismuto, que muitas vezes impedem a adesão ao tratamento, como: paladar amargo, vômitos e náusea 23,24 .…”

“…E ainda há como, ponto positivo, para o uso de cepas de Lactobacilos, a redução das reações causados pela terapia com antibióticos e bismuto, que muitas vezes impedem a adesão ao tratamento, como: paladar amargo, vômitos e náusea 23,24 . Alguns estudos demonstraram a melhora de patologias causadas pela infecção da H. pylori, como gastrite e úlcera péptica 17,30 . Embora, alguns estudos demonstraram que a redução de sinais e sintomas tenham sido ligeiramente menor em pacientes tratados com as cepas probióticas de Lactobacilos 26 ou apresentado melhora apenas em um único tipo de efeito, o paladar amargo 25 .…”