The Anti-Proliferation Activity and Mechanism of Action of K12[V18O42(H2O)]∙6H2O on Breast Cancer Cell Lines

Abstract

Polyoxometalates (POMs) are inorganic clusters that possess potential anti-bacterial, anti-viral, and anti-tumor activities. Herein, the in vitro anti-proliferation activities of K12[V18O42(H2O)]∙6H2O (V18) have been investigated on the MCF-7 and MDA-MB-231 cell lines. The results indicated that V18 could inhibit the proliferation of MCF-7 (IC50, 11.95 μM at 48 h) in a dose-dependent manner compared to the positive control, 5-fluorouracil (5-Fu, p < 0.05). The anti-proliferation activity of V18 might be mediated by arrest of the MCF-7 cells in the G2/M phase and induction of apoptosis and necrosis. Moreover, V18 can effectively quench the fluorescence of ctDNA. The binding mode between them may be groove or outside stacking binding. V18 can also effectively quench the intrinsic fluorescence of bovine serum albumin (BSA) and human serum albumin (HSA) via static quenching, and changed the conformation of BSA and HSA.

Stabilization and Binding of [V4O12]4− and Unprecedented [V20O54(NO3)]n− to Lysozyme upon Loss of Ligands and Oxidation of the Potential Drug VIVO(acetylacetonato)2

“…These present various combinations of V IV and V V centres, [36] from which the value of the charge nÀ , the structural features and the magnetic, [37] catalytic, [49,50] and biological properties depend. [46,51] These POVs are able to incorporate in the centre of the cage various types of anions such as Cl À , Br À , I À , HS À , HCOO À , CO 3 2À , N 3 À , NO 2 À , NO 3 À or, also, neutral species like water. To the best of our knowledge, the cage observed in our structure is the second one with composition V 18 O 46 , after [V V 16 V IV 2 O 46 (NO 3 )] 5À .…”

“…Instead, in literature, five types of octadecavanadate (V 18 ) cages with composition [V 18 O 39 ] n− , [34] [V 18 O 41 ] n− , [35] [V 18 O 42 ] n− , [36–45] [V 18 O 44 ] n− , [46,47] and [V 18 O 46 ] n− , [48] have been reported. These present various combinations of V IV and V V centres, [36] from which the value of the charge n−, the structural features and the magnetic, [37] catalytic, [49,50] and biological properties depend [46,51] . These POVs are able to incorporate in the centre of the cage various types of anions such as Cl − , Br − , I − , HS − , HCOO − , CO 3 2− , N 3 − , NO 2 − , NO 3 − or, also, neutral species like water.…”

The Future Is Bright for Polyoxometalates

“…The anti-proliferation activity of another POV, V 18 , was observed to affect the cellular cycle, and to mediate the arrest of MCF-7 cells in the G2/M phase and induction of apoptosis, besides DNA-, BSA-, and HSA-binding [85]. POV studies were also performed with U-87 and human liver SMMC-7721 cancer cells, and cell cycle arrest, DNA damage, and apoptosis were observed [85,94].…”

“…Similarly, POT and POMos studies together represent the majority (90%), whereas for POVs and PONbs, lower percentages can be found. In fact, more than 120 articles/papers have been published with POMos and polyoxotungstates POTs in antitumor studies [8,[10][11][12][13][80][81][82][83][84][85][86][87][88][89]. POMs in cancer therapy and diagnostics, their modes of action, and future perspectives were reviewed [2,[90][91][92][93].…”

In Vitro, Oral Acute, and Repeated 28-Day Oral Dose Toxicity of a Mixed-Valence Polyoxovanadate Cluster

“…Mixed-valence polyoxovanadates (MVPs) have contributed to an increase in the number of potential therapeutic agents for metabolic diseases and cancer [ 25 , 26 , 27 , 28 ]. The MVP [(Me 4 N) 6 [V 15 O 36 Cl] is a polyoxovanadate (abbreviated V 15 , see Figure 1 ) that contains six Me 4 N + counterions, and the vanadium-oxygen cage of V 15 consists of one central chlorine atom, eight vanadium(IV) and seven vanadium(V) atoms, and 36 oxygen atoms ( Figure 1 ).…”