The anti-proliferative agent jasplakinolide rearranges the actin cytoskeleton of plant cells

Sawitzky, Heiko; Liebe, Susanne; Willingale−Theune, Julia; Menzel, Diedrik

doi:10.1016/s0171-9335(99)80085-5

Cited by 33 publications

(29 citation statements)

References 36 publications

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“…A broad array of experiments have demonstrated that jasplakinolide modulates actin polymerization (2,12,22,24). It is interesting to consider that the well-documented antiproliferative activity of jasplakinolide may correlate with reorganization of the actin cytoskeleton.…”

Section: Discussionmentioning

confidence: 99%

“…The results imply that jasplakinolide exerts its cytotoxic effect by inducing actin polymerization and/or inhibiting the depolymerization of actin filaments. In fact, it was demonstrated that exposure of living cells to jasplakinolide results in the formation of multinucleated cells and disruption of actin in these cells (12,22,24). Recently, this cell-permeable drug has been used to investigate the involvement of actin microfilaments in a variety of cellular processes, including cell movement, integrin-mediated adhesion, oocyte maturation, and transport in the endocytic pathways, including protein trafficking in the Golgi apparatus (4,9,11,18,25,26,28,31).…”

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confidence: 99%

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Jasplakinolide Induces Apoptosis in Various Transformed Cell Lines by a Caspase-3-Like Protease-Dependent Pathway

Odaka

Sanders

Crews

2000

Clin Diagn Lab Immunol

125

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Although it has been well established that murine immature thymocytes are sensitive to apoptosis when exposed to various apoptotic stimuli, these cells as well as mature T lymphocytes were resistant to jasplakinolide-induced apoptosis. The results suggest that jasplakinolide induces apoptotic cell death through a caspase-3-like protease-dependent pathway. Another important outcome is that transformed cell lines were more susceptible to jasplakinolide-induced apoptosis than normal nontransformed cells.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Jasplakinolide Induces Apoptosis in Various Transformed Cell Lines by a Caspase-3-Like Protease-Dependent Pathway

Odaka

Sanders

Crews

2000

Clin Diagn Lab Immunol

125

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“…al. 2001] or stabilize actin such as Jasplakinolide [Sawitzky et al, 1999;Ou et al, 2002]. To further characterize the nature of the filaments decorated by the BD1/2-GFP fusions, we treated roots expressing BD1/2-GFP with Lat B or Jasplakinolide.…”

Section: Bd1/2-gfp Expression Revealed a Dynamic Factin Network In Ramentioning

confidence: 99%

Green fluorescent protein fusions to Arabidopsis fimbrin 1 for spatio‐temporal imaging of F‐actin dynamics in roots

Wang

Motes

Mohamalawari

et al. 2004

Cell Motil. Cytoskeleton

116

107

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The visualization of green fluorescent protein (GFP) fusions with microtubule or actin filament (F-actin) binding proteins has provided new insights into the function of the cytoskeleton during plant development. For studies on actin, GFP fusions to talin have been the most generally used reporters. Although GFP-Talin has allowed in vivo F-actin imaging in a variety of plant cells, its utility in monitoring F-actin in stably transformed plants is limited particularly in developing roots where interesting actin dependent cell processes are occurring. In this study, we created a variety of GFP fusions to Arabidopsis Fimbrin 1 (AtFim1) to explore their utility for in vivo F-actin imaging in root cells and to better understand the actin binding properties of AtFim1 in living plant cells. Translational fusions of GFP to full-length AtFim1 or to some truncated variants of AtFim1 showed filamentous labeling in transient expression assays. One truncated fimbrin-GFP fusion was capable of labeling distinct filaments in stably transformed Arabidopsis roots. The filaments decorated by this construct were highly dynamic in growing root hairs and elongating root cells and were sensitive to actin disrupting drugs. Therefore, the fimbrin-GFP reporters we describe in this study provide additional tools for studying the actin cytoskeleton during root cell development. Moreover, the localization of AtFim1-GFP offers insights into the regulation of actin organization in developing roots by this class of actin cross-linking proteins.

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“…These changes are often mediated by dynamic cytoskeleton, the network of microfilaments, microtubules as well as their associated proteins (Fowler & Quatrano, 1997;Kropf et al, 1998). Most traditional studies of actin in a specific cellular response have been focused on cytoskeletal inhibitors including stabilizing agents (cytochalasins, phalloidin and jasplakinolide) (Sawitsky et al, 1999). Depolymerizing compounds or disrupters of actin polymerzation (macrolide and potassium iodide -KI) are also involved (La Claire, 1989;Shimmen et al, 1995;Sawitsky, et al, 1999;Spector et al, 1999).…”

Section: Produção De Látex Estimulada Pela Aplicação De Compostos Desmentioning

confidence: 99%

“…Most traditional studies of actin in a specific cellular response have been focused on cytoskeletal inhibitors including stabilizing agents (cytochalasins, phalloidin and jasplakinolide) (Sawitsky et al, 1999). Depolymerizing compounds or disrupters of actin polymerzation (macrolide and potassium iodide -KI) are also involved (La Claire, 1989;Shimmen et al, 1995;Sawitsky, et al, 1999;Spector et al, 1999). Natural rubber from rubber trees is strategically and widely used in military and civil industries.…”

Section: Produção De Látex Estimulada Pela Aplicação De Compostos Desmentioning

confidence: 99%

Latex production with depolymerizing compounds of actin cytoskeleton in rubber trees

Gao

Meng

2008

Pesq. agropec. bras.

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-The objective of this work was to assess stimulated latex flow from rubber trees (Hevea brasiliensis) with saturated macrolide (latrunculin A), 1, 5, and 10% potassium iodide in 2% methylcellulose compared with 0.3% ethylene in 2% methylcellulose (check) and 2% methylcellulose (blank). Latex output and contents of pure rubber, total solid, sucrose, inorganic phosphorus, thiol, and Mg 2+ were measured. The treatments containing 1% KI or saturated macrolide increased latex yields compared to the blank with 2% methylcellulose alone. The 1% KI or saturated macrolide treatments were equal to that of 0.3% ethylene check treatment. However, 5 and 10% KI were harmful to bark of rubber trees, even caused prolonged tapping panel dryness.Index terms: ethylene, latex yields, macrolide, microfilaments, physiological parameters, potassium iodide. Produção de látex estimulada pela aplicação de compostos despolimerizantes de citoesqueleto de actina em seringueirasResumo -O objetivo deste trabalho foi avaliar a produção de látex em seringueiras (Hevea brasiliensis) estimulada pela aplicação de macrolídeo saturado (latrunculina A), iodeto de potássio a 1, 5 e 10%, e etileno a 0,3%, preparados em solução de metilcelulose a 0,2%. O tratamento testemunha consistiu da aplicação de solução pura de metilcelulose a 2%. Foram medidos a produção de látex e o conteúdo de borracha pura, sólidos totais, sacarose, fósforo inorgânico, tiol e Mg 2+ . A aplicação de KI a 1% ou de macrolídeo saturado aumentou a produção de látex em comparação com o tratamento testemunha. Os tratamentos com KI a 1% e macrolídeo saturado foram iguais ao tratamento com etileno a 0,3%. No entanto, os tratamentos com KI a 5 e 10% foram prejudiciais à casca da seringueira, causando até mesmo o secamento do painel-de-sangria.

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The anti-proliferative agent jasplakinolide rearranges the actin cytoskeleton of plant cells

Cited by 33 publications

References 36 publications

Jasplakinolide Induces Apoptosis in Various Transformed Cell Lines by a Caspase-3-Like Protease-Dependent Pathway

Jasplakinolide Induces Apoptosis in Various Transformed Cell Lines by a Caspase-3-Like Protease-Dependent Pathway

Green fluorescent protein fusions to Arabidopsis fimbrin 1 for spatio‐temporal imaging of F‐actin dynamics in roots

Latex production with depolymerizing compounds of actin cytoskeleton in rubber trees

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