The antiangiogenic effects of tyroservatide on animal models of hepatocellular carcinoma

Zhang, Ning; Wang, Lu; Li, Ying; Zhao, Yiming; Qin, Xue; Wu, Wei‐Zhong; Sun, Hui‐Chuan; Fan, Jia; Tang, Zhao–You

doi:10.1007/s00432-009-0589-1

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…1B, and top ve mutated genes were BCLAF1, MUC4, TP53, FMN2 and TTC7A with mutation prevalence rates of 73%, 57%, 49%, 49% and 46% in our cohort, respectively. The results are basically consistent with previous research reports [16,17]. Moreover, we identi ed 32 differential genes (p < 0.05, Supplementary table 1) between two groups, of which 25 genes were only detected in the recurrence group (Fig.…”

Section: Clinical Characteristics Analysis Of Recurrence Group and No...supporting

confidence: 92%

Genomic alterations in hepatocellular carcinoma patients undergoing liver transplantation predict recurrence and prognosis

Li,

Wang,

et al. 2024

Preprint

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Liver transplantation (LT) stands as a pivotal treatment for hepatocellular carcinoma (HCC), outperforming comprehensive treatments in long-term efficacy. However, the 5-year post-LT survival rate hovers between 60% and 70%, largely due to recurrent HCC, spotlighting the critical need for biomarkers that can predict recurrence and prognosis following LT. Our study embarked on this challenge by retrospectively analyzing data from 37 HCC patients who underwent LT from January 2019 to January 2021. Employing whole exome sequencing on tissue and control blood samples, we segregated these patients into recurrence (n = 14) and non-recurrence groups (n = 23), based on a one-year postoperative threshold. Our analysis unveiled a distinctive genomic mutation spectrum in these patients, highlighting five predominantly mutated genes: BCLAF1, MUC4, TP53, FMN2, and TTC7A. Notably, clinical features between the two groups showed no significant divergence. However, the recurrence group demonstrated markedly inferior overall survival (OS) compared to their counterparts (p < 0.0001). Multivariate regression pinpointed 304 genes as independent predictors for recurrence-free survival (RFS) and 482 genes for OS (p < 0.05). Additionally, our research led to the development of a novel 13-gene model, which markedly influences both RFS and OS. Patients classified within the high-risk category of this model experienced significantly poorer outcomes. This study is a trailblazer in linking genomic alterations with the recurrence and survival rates of HCC patients post-LT, introducing a 13-gene model that offers substantial predictive and prognostic utility.

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Section: Clinical Characteristics Analysis Of Recurrence Group and No...supporting

confidence: 92%

Genomic alterations in hepatocellular carcinoma patients undergoing liver transplantation predict recurrence and prognosis

Li,

Wang,

et al. 2024

Preprint

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“…Studies in animal tumor models demonstrate that the treatment with antiangiogenic agents is associated with a decrease in MVD ( 20,21 ). Thus, the assessment of MVD in biopsy samples taken over the course of tumor treatment is a straightforward strategy to monitor the effectiveness of antiangiogenic therapy and is commonly used in many antiangiogenic tumor treatment studies ( 22 ).…”

Section: Animal Modelmentioning

confidence: 99%

Quantitative Assessment of Tumor Blood Flow in Mice after Treatment with Different Doses of an Antiangiogenic Agent with Contrast-enhanced Destruction-Replenishment US

Zhou¹,

Cao²,

Liu³

et al. 2011

Radiology

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“…The final volume of each RT-PCR was 25 ll consisting of 12.5 ll of iQ TM SYBR Green PCR master mix, 5 ll of obtained cDNA (2 ng/ll; or water as a negative control), nuclear-free water (Ambion), as well as sense and anti-sense primers designed specifically for quantitative RT-PCR and having similar efficiencies (Bahrami et al 2011;Capasso et al 2012;Nahidi et al 2012;Watanabe et al 2013;Zhang et al 2009) (Table 1). Cycling temperatures for the RT-PCRs were as follows: initial hot start step of 2 min at 95°C for all reactions, and then 40 cycles of 95°C for 20 s, 63°C for 30 s and 72°C for 60 s. To ensure the specificity of amplification, each cycling programme was followed by a melting phase, and each melting curve was checked for the presence of a single peak to demonstrate a single amplification product.…”

Section: Rna Extraction and Amplification By Real-time Pcr (Rt-pcr)mentioning

confidence: 99%

The major pathway by which polymeric formula reduces inflammation in intestinal epithelial cells: a microarray-based analysis

et al. 2015

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Nutritional therapy is well established as a means to induce remission in active Crohn's disease (CD). Evidence indicates that exclusive enteral nutrition (EEN) therapy for CD both alters the intestinal microbiota and directly suppresses the inflammatory response in the intestinal mucosa. However, the pathway(s) through which EEN suppresses inflammation is still unknown. Therefore, the aim of the current study was to use microarray technology to investigate the major pathway by which polymeric formula (PF) alters inflammatory processes in epithelial cells in vitro. HT-29 cells were grown to confluence and then co-cultured with tumour necrosis factor (TNF)-a (100 ng/ml) for 5 h in the presence or absence of PF, as used for EEN. Following incubation, RNA was extracted and subjected to polymerase chain reaction (PCR) and microarray analysis. Enzyme-linked immunosorbent assays were employed to evaluate cytokine protein levels. Neither TNF-a nor PF had a toxic effect on cells over the experimental period. Microarray analysis showed that PF modulated the expression of genes specifically linked to nuclear factor (NF)-jB, resulting in downregulation of a number of genes in this pathway. These findings were further confirmed by real-time PCR of selected dysregulated genes as well as reduced expression of IL-6 and IL-8 proteins following PF treatment. The results arising from this study provide evidence that PF alters the inflammatory responses in intestinal epithelial cells through modulation of the NF-jB pathway.

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The antiangiogenic effects of tyroservatide on animal models of hepatocellular carcinoma

Abstract: YSV could inhibit the growth of human hepatocarcinoma SMMC-7721 tumor in nude mice, which might attribute to the inhibition of expression of angiogenesis-related factors in mRNA and protein level.

Cited by 5 publications

References 19 publications

Genomic alterations in hepatocellular carcinoma patients undergoing liver transplantation predict recurrence and prognosis

Genomic alterations in hepatocellular carcinoma patients undergoing liver transplantation predict recurrence and prognosis

Quantitative Assessment of Tumor Blood Flow in Mice after Treatment with Different Doses of an Antiangiogenic Agent with Contrast-enhanced Destruction-Replenishment US

The major pathway by which polymeric formula reduces inflammation in intestinal epithelial cells: a microarray-based analysis

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