The antibacterial activity and modes of LI-F type antimicrobial peptides against<i>Bacillus cereus in vitro</i>

Han, Jun; Zhao, Shengming; Ma, Zhi; Gao, Ling; Liu, Hongxia; Umair, Muhammad; Lu, Zhaoxin; Lv, Fengting; Bie, Xiaomei

doi:10.1111/jam.13526

Cited by 23 publications

(12 citation statements)

References 51 publications

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“…These results demonstrated that both plantaricin GZ1-27 and nisin increased the permeability of the B. cereus cell membrane, which led to the efflux of K + . Similar results were reported for antimicrobial peptide F1, 32 antimicrobial peptide AMP-jsa9, 20 lactosporin, 33 and two-peptide (LtnA1 and LtnA2). 34 Effect of Plantaricin GZ1-27 on the Integrity and Viability of B. cereus Cells.…”

Section: ■ Results and Discussionsupporting

confidence: 88%

“…After centrifugation at 5000 × g for 10 min (4 °C), the supernatants were filtered through 0.22 μm filters and the K + concentrations were measured on an Optima 8000 plasma atomic emission spectrometry (PerkinElmer Inc., Waltham, MA). 20 Flow Cytometry. Bacterial membrane integrity was assayed by measuring the uptake of propidium iodide.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Purification, Characterization, and Mode of Action of Plantaricin GZ1-27, a Novel Bacteriocin against Bacillus cereus

Yang

et al. 2018

J. Agric. Food Chem.

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Bacillus cereus is an opportunistic pathogen that causes foodborne diseases. We isolated a novel bacteriocin, designated plantaricin GZ1-27, and elucidated its mode of action against B. cereus. Plantaricin GZ1-27 was purified using ammonium sulfate precipitation, gel-filtration chromatography, and RP-HPLC. MALDI-TOF/MS revealed that its molecular mass was 975 Da, and Q-TOF-MS/MS analysis predicted the amino acid sequence as VSGPAGPPGTH. Plantaricin GZ1-27 showed thermostability and pH stability. The antibacterial mechanism was investigated using flow cytometry, confocal laser-scanning microscopy, scanning and transmission electron microscopy, and RT-PCR, which revealed that GZ1-27 increased cell membrane permeability, triggered K leakage and pore formation, damaged cell membrane integrity, altered cell morphology and intracellular organization, and reduced the expression of genes related to cytotoxin production, peptidoglycan synthesis, and cell division. These results suggest that plantaricin GZ1-27 effectively inhibits B. cereus at both the cellular and the molecular levels and is a potential natural food preservative targeting B. cereus.

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Section: ■ Results and Discussionsupporting

confidence: 88%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Purification, Characterization, and Mode of Action of Plantaricin GZ1-27, a Novel Bacteriocin against Bacillus cereus

Yang

et al. 2018

J. Agric. Food Chem.

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“…The SEM examination was carried out using the method of Han et al. (2017). Compound 3 was treated with B .…”

Section: Methodsmentioning

confidence: 99%

LC‐ESI‐QTOF/MS characterization of antimicrobial compounds with their action mode extracted from vine tea (Ampelopsis grossedentata) leaves

Umair

Sultana

Zhu

et al. 2022

Food Science & Nutrition

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Vine tea (Ampelopsis grossedentata) is a tea plant cultivated south of the Chinese Yangtze River. It has anti‐inflammatory properties and is used to normalize blood circulation and detoxification. The leaves of vine tea are the most abundant source of flavonoids, such as dihydromyricetin and myricetin. However, as the main bioactive flavonoid in vine tea, dihydromyricetin was the main focus of previous research. This study aimed to explore the antibacterial activities of vine tea against selected foodborne pathogens. The antimicrobial activity of vine tea extract was evaluated by the agar well diffusion method. Cell membrane integrity and bactericidal kinetics, along with physical damage to the cell membrane, were also observed. The extract was analyzed using a high‐performance liquid chromatography‐diode array detector (HPLC‐DAD), and the results were confirmed using a modified version of a previously published method that combined liquid chromatography and electrospray‐ionized quadrupole time‐of‐flight mass spectrometry (LC‐ESI‐QTOF/MS). Cell membrane integrity and bactericidal kinetics were determined by releasing intracellular material in suspension and monitoring it at 260 nm using an ultraviolet (UV) spectrophotometer. A scanning electron microscope (SEM) was used to detect morphological alterations and physical damage to the cell membrane. Six compounds were isolated successfully: (1) myricetin (C15H10O8), (2) myricetin 3‐O‐rhamnoside (C21H20O12), (3) 5,7,8,3,4‐pentahydroxyisoflavone (C15H10O7), (4) dihydroquercetin (C15H12O7), (5) 6,8‐dihydroxykaempferol (C15H10O8), and (6) ellagic acid glucoside (C20H16O13). Among these bioactive compounds, C15H10O7 was found to have vigorous antimicrobial activity against Bacillus cereus (AS11846) and Staphylococcus aureus (CMCCB26003). A dose‐dependent bactericidal kinetics with a higher degree of absorbance at optical density 260 (OD260) was observed when the bacterial suspension was incubated with C15H10O7 for 8 h. Furthermore, a scanning electron microscope study revealed physical damage to the cell membrane. In addition, the action mode of C15H10O7 was on the cell wall of the target microorganism. Together, these results suggest that C15H10O7 has vigorous antimicrobial activity and can be used as a potent antimicrobial agent in the food processing industry.

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“…The viable counts of tested bacteria were determined based on J Han's detection method (Han et al, 2017). The FC at the MIC level was used to inoculate a bacterial suspension containing 10 7 CFU/ml and incubate at 37 °C with a 150 r/min shaking incubator for viable bacteria count.…”

Section: Antibacterial Potentialmentioning

confidence: 99%

UPLC/Q-TOF MS Screening and Identification of Antibacterial Compounds in Forsythia suspensa (Thunb.) Vahl Leaves

Zhou

Huo²,

Wang

et al. 2022

Front. Pharmacol.

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Forsythia suspensa (Thunb.) Vahl (F. suspensa) is a traditional Chinese medical herb and only its fruit is currently used in clinical therapies. However, the discarded parts like leaves also contain a large number of active components. In this study, we used macroporous adsorption resin to enrich the effective components from F. suspensa leaves. The separated active compounds were then identified and quantified by ultra-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC/Q-TOF MS) and high-performance liquid chromatography Active components with antibacterial properties extracted from F. suspensa leaves were confirmed in vitro and the corresponding mechanisms were explored. In sum, a stable and effective method for extracting antibacterial active components from F. suspensa leaves was established in this study, which proved the practicability of F. suspensa leaves as traditional Chinese medicine and is conducive to the more comprehensive utilization of the plant.

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The antibacterial activity and modes of LI-F type antimicrobial peptides againstBacillus cereus in vitro

Cited by 23 publications

References 51 publications

Purification, Characterization, and Mode of Action of Plantaricin GZ1-27, a Novel Bacteriocin against Bacillus cereus

Purification, Characterization, and Mode of Action of Plantaricin GZ1-27, a Novel Bacteriocin against Bacillus cereus

LC‐ESI‐QTOF/MS characterization of antimicrobial compounds with their action mode extracted from vine tea (Ampelopsis grossedentata) leaves

UPLC/Q-TOF MS Screening and Identification of Antibacterial Compounds in Forsythia suspensa (Thunb.) Vahl Leaves

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