The antibacterial activity of contact lens solutions against microbial keratitis

Budiman, Arif; Fauzi, Haniq Juniswapy; Sulistiyaningsih, Rr; Sriwidodo, Sriwidodo

doi:10.5455/njppp.2017.7.0724228072017

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…However, to derive a conclusion, a more number of isolates are recommended and even other types of genes are also screened. [15,16] This indicates the important role of clinical microbiology laboratories to distinguish MBL-producing P. aeruginosa from strains with other mechanisms responsible for carbapenem resistance.…”

Section: Discussionmentioning

confidence: 99%

Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa

Raj¹,

Pa²,

Jain³

2018

Natl J Physiol Pharm Pharmacol

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Background: In recent decades, Pseudomonas aeruginosa has emerged as a multidrug-resistant bacteria by acquiring intrinsic resistance to a number of antimicrobial agents by uses distinctive resistant mechanisms to all the available antibiotics, which include metallo-β-lactamases (MBL) production, extended spectrum β-lactamase production, Amp C production, decreased permeability, altered penicillin binding proteins and rarely, and over expression of efflux pumps. Aims and Objectives: The aim of the study was to know the presence of IMP gene among P. aeruginosa isolates and to determine the carbapenem resistance mechanisms in carbapenem-resistant isolates of P. aeruginosa collected from sputum, blood, urine, pus, in Chennai, India. Materials and Methods: A total of 20 of non-repetitive clinical isolates of P. aeruginosa were collected and processed for biochemical tests and confirmed. Antibiotic susceptibility testing was determined by Kirby Bauer disc diffusion method. P. aeruginosa isolates were detected for the presence of bla IMP gene by polymerase chain reaction analysis. Results: Of the 20 clinical isolates of P. aeruginosa, 9/20 (45%) isolates were from sputum, 5/20 (25%) from blood, 3/20 (15%) from urine, and 3/20 (15%) from pus. Only 2/20 (10%) isolates showed sensitivity to imipenem. Other than that, for all other antibiotics isolates showed complete resistance 20/20 (100%). 17/20 (85%) clinical isolate of P. aeruginosa was found to possess bla IMP gene. Conclusion: The early detection of MBL-producing P. aeruginosa may help in appropriate antimicrobial therapy and avoid the development and dissemination of these multidrug-resistant strains. However, to derive a conclusion, a more number of isolates are recommended and even other types of genes are also screened.

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Section: Discussionmentioning

confidence: 99%

Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa

Raj¹,

Pa²,

Jain³

2018

Natl J Physiol Pharm Pharmacol

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“…The phenol content of black mulberry fruit can provide pharmacological activities such as antioxidant 13 , antibacterial 14 , hypolipidemic effect 15 and macrophage activation effect 16 . The highest concentration of phenol can be found in black mulberry fruit (Morus nigra L) 17 , and with this high phenolic content black mulberry has potential for antioxidant activity 1,18 .…”

Section: Chemical Constituents and Pharmacological Activitymentioning

confidence: 99%

Chemical Compounds and Pharmacological Activity of Morus Nigra as a Potential Product of Drug: A Review

Budiman¹,

Sulastri²,

Qoriah³

2018

Int. Res. J. Pharm.

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Black mulberry fruit (Morus nigra L.) is spread over the subtropical regions of the earth and can grow in various topography and climatic conditions. Its fruit mostly proceeds to become juice, jam, coloring agent and a flavoring for ice cream. Traditionally, the leaves and fruit of Morus nigra L. are used to treat dysentery, diabetes mellitus, hypertension, arthritis, and anemia and can also act as a laxative, odontalgia, anthelmintic, expectorant, hypoglycemic and emetic agent. These pharmacological activities might be caused by a high amount of active compounds in black mulberry. Based on the literature studies, there are 56 compounds isolated from various parts of black mulberry, including the root bark, roots, bark, wood, leaves and fruit. The chemical constituents found in black mulberry contribute to its pharmacological properties, such as antihyperglycemic, cytotoxic against leukemia cells, antioxidative, antibacterial, anti-inflammatory and inhibition of tyrosinase.

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“…Manufacturers recommend a minimum of 6 h contact with active MPDS agents to affect cell viability. [ 16 ] The effect of this is to disturb the microbial capacity for carrying out all cellular functions required for lens maintenance under normal living conditions. [ 17 ]…”

Section: Introductionmentioning

confidence: 99%

Multi-purpose Disinfecting Solutions only Partially Inhibit the Development of Ocular Microbes Biofilms in Contact Lens Storage Cases

Araújo

Morais

Oliveira

et al. 2021

Middle East African Journal of Ophthalmology

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PURPOSE: Certain ocular resident or pathogenic microbes may remain viable in the presence of multi-purpose disinfectant solutions (MPDSs), subsequently developing biofilms inside contact lens storage cases (CLSCs) which pose a risk of infection to wearers. This study evaluated the formation of ocular microbiota biofilms exposed to three top selling MPDS. METHODS: Crystal violet assay was carried out for the verification of biofilm formation. The in vitro assays evaluated Pseudomonas aeruginosa UFPEDA 416 and Staphylococcus aureus UFPEDA 02 exposure of 48 h to MPDS, as well as the use of 40 KHz ultrasound at the beginning and with 24 h immersion in the MPDS. Subsequently, in vivo assays evaluated the formation of microbial biofilms on the CLSC walls containing silicone-hydrogel contact lenses immersed in MPDS from 15 healthy volunteer patients, who had been wearing the lenses for 7 days. RESULTS: Biofilms were inhibited by 26%–98% in the in vitro assays, with a statistically significant difference only for P. aeruginosa UFPEDA 416 exposed to diluted MPDS. Most inhibitions occurred moderately and weakly. In addition, adherent cells were detected in more than 90% of the tests. Biofilm was not inhibited in more than one third of the results, nor was it disturbed, especially with the ultrasound treatments. The average of obtained optical densities at 590 nm was between 0.6 and 0.8 in the in vivo assays. The results were similar between the CLSC right and left wells. There was a correlation between microbial biofilm formation and the type of MPDS tested, with statistical difference between the three treatments. CONCLUSION: MPDS promoted a partial inhibition of microbial biofilm formation but only one MPDS proved to be more effective in vitro and in vivo. This study, however, could not distinguish the effect of possible errors in the good hygiene practices of the users.

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The antibacterial activity of contact lens solutions against microbial keratitis

Cited by 3 publications

References 9 publications

Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa

Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa

Chemical Compounds and Pharmacological Activity of Morus Nigra as a Potential Product of Drug: A Review

Multi-purpose Disinfecting Solutions only Partially Inhibit the Development of Ocular Microbes Biofilms in Contact Lens Storage Cases

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