The antibacterial activity of mineral trioxide aggregate containing calcium fluoride

Lim, Miyoung; Yoo, Seung-Hoon

doi:10.1016/j.jds.2021.09.005

Cited by 14 publications

(11 citation statements)

References 35 publications

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“…This could be due to the presence of calcium silicates, which enhanced the formation of calcium hydroxide that had an acidic pH causing a bactericidal effect [ 15 ]. Also, the addition of calcium fluoride enhances the antimicrobial action of the cement [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

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Histologic Assessment of a Fast-Set Mineral Trioxide Aggregate (MTA) and Two Novel Antibacterial-Enhanced Fast-Set MTAs for Apexification and Periapical Healing of Teeth With Incomplete Root Formation in a Rat Model: An In Vivo Animal Study

Fathima,

Ravindran,

Jeevanandan

et al. 2024

Cureus

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Background Pulp necrosis in incomplete root formation halts dentine development, resulting in larger canals with fragile walls and an open apex, complicating canal instrumentation and apical stop formation. Bioactive endodontic cements such as mineral trioxide aggregate (MTA) are crucial for creating artificial apical barriers or inducing apical foramen closure, but challenges remain regarding their antimicrobial efficacy and cytotoxicity. Modifications to MTA formulations aim to address these concerns. Methods This in vivo animal study involved 80 Wistar albino rats, with incomplete root formation induced by pulp exposure. Rats were divided into four groups receiving different MTA formulations for apexification: conventional MTA, modified MTA, and MTA enhanced with metronidazole or doxycycline. Histopathological evaluations were conducted at seven and 28 days post-treatment to assess calcific barrier formation, inflammatory reactions, and antimicrobial efficacy. Results By day 7, modified MTA formulations exhibited enhanced antibacterial activity compared to conventional MTA (p = 0.000), with fewer inflammatory reactions and microorganisms. By day 28, modified formulations showed superior calcific barrier formation, particularly in the metronidazole- and doxycycline-enhanced groups compared to conventional MTA (p = 0.000). These outcomes suggest that modifications to MTA formulations improve antimicrobial efficacy and calcific barrier formation in vivo. Conclusion Novel modified MTA formulations, particularly those enhanced with metronidazole or doxycycline, exhibit superior antibacterial efficacy and calcific barrier formation compared to conventional MTA. Further long-term studies are warranted to validate these findings for potential clinical translation.

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Section: Discussionmentioning

confidence: 99%

“…Enterococcus faecalis stands out as the most commonly encountered bacterium in stubborn infections post-endodontic therapy [ 5 ]. Prior investigations have yielded contradictory findings concerning MTA's ability to combat E. faecalis .…”

Section: Introductionmentioning

confidence: 99%

Histologic Assessment of a Fast-Set Mineral Trioxide Aggregate (MTA) and Two Novel Antibacterial-Enhanced Fast-Set MTAs for Apexification and Periapical Healing of Teeth With Incomplete Root Formation in a Rat Model: An In Vivo Animal Study

Fathima,

Ravindran,

Jeevanandan

et al. 2024

Cureus

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“…The acknowledgment of low to moderate antimicrobial activity against E. faecalis in conventional MTA underscores the importance of addressing this limitation [ 12 ]. The addition of agents like calcium fluoride and fluorohydroxyapatite to enhance the antimicrobial activity of MTA is a well-founded strategy [ 13 , 14 ]. The inclusion of 5 wt% of doxycycline and metronidazole in groups 2 and 3, respectively, in the present study reflects a targeted approach to improving the antimicrobial properties of the modified MTA.…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Push-Out Bond Strength of Conventional Mineral Trioxide Aggregate, Biodentine, a Modified Mineral Trioxide Aggregate, and Two Novel Antibacterial-Enhanced Mineral Trioxide Aggregates

Shancy Merlin,

Ravindran,

Jeevanandan

et al. 2024

Cureus

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Background The challenges associated with incorporating antimicrobial agents, such as the potential diminishment of the cement's physical properties, highlight the need for comprehensive evaluations. Balancing antimicrobial efficacy with the maintenance of structural integrity is a crucial aspect of material development. The acknowledgment of cytotoxic properties associated with tricalcium aluminate, a major constituent in conventional mineral trioxide aggregate (MTA), is critical in terms of long-term evaluation of treatment procedures. The primary focus of the push-out test is to evaluate the resistance of the tested material to dislodgement. Greater push-out strength implies stronger adhesion between the tested material and the tooth surface. Aim This study aims to evaluate the push-out bond strength of two antibacterial-enhanced MTAs with conventional MTA and Biodentine. Material and methods A total of five materials were tested: a) modified MTA, b) doxycycline-enhanced MTA, c) metronidazole-enhanced MTA, d) conventional MTA, and e) Biodentine. All the materials were mixed based on a predetermined powder:liquid ratio and then carried using a plastic instrument to the desired experimental design. Single-rooted permanent teeth, preferably incisors, were used in the present study. Teeth were embedded vertically in a rubber mold, and sectioning of the tooth was performed. A single operator instrumented the canal space in each slice using Gates-Glidden burs, and the mixed cements were placed in the respective groups and stored for 72 hours. A push-out test was carried out using a universal testing machine. Following the bond failure, the slices were examined under a stereomicroscope to determine the nature of the bond failure. The collected data was subjected to a one-way analysis of variance test, post hoc test, and chi-square test for statistical analysis. Results The mean push-out bond strength was found to be the highest for Biodentine (43.25 ± 0.62 megapascals (MPa)), followed by doxycycline- and metronidazole-enhanced MTAs (39.54 ± 0.65 MPa and 39.29 ± 0.16 MPa, respectively), modified MTA formulation (37.75 ± 0.73 MPa), and the lowest for conventional MTA (25.93 ± 0.7 MPa). Conventional MTA samples had an adhesive failure (89.4%), while Biodentine samples had a cohesive failure (80.3%). Mixed failures were noticed with the samples containing modified MTA formulation (71.3%), doxycycline-enhanced MTA (76.6%), and metronidazole-enhanced MTA (78.0%). Conclusion Despite not surpassing Biodentine in bond strength, antibacterial-enhanced MTAs are considered potential alternatives to conventional MTA in day-to-day clinical practice.

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“…CuNP, an antibacterial agent [34], is still active even though combined with MTA. The antibacterial performance mechanism is probably different from MTA modified with CaF 2 [14] and fluorohydroxiapatite [15], in which the higher pH leads to bacterial death. The CuNP on the MTA surface may interact with the bacterial cells causing bacterial cell leakage and leading to lysed and death [35].…”

Section: Characteristics Of Cunpmentioning

confidence: 94%

“…Yuliatun et al [13] reported the addition of SrO 5% and hydroxyapatite 6% increased the compressive strength of MTA from 2.21 to 18.01 MPa and improved the adhesion between dentin surface and MTA. Lim and Yoo [14] reported the modification of MTA with calcium fluoride (CaF 2 ) to give antibacterial activity against Enterococcus faecalis, Porphyromonas endodontalis, and Porphyromonas gingivalis. Bolhari et al [15] improved the antibacterial activity of MTA by mixing MTA with fluorohydroxyapatite against E. faecalis.…”

Section: ■ Introductionmentioning

confidence: 99%

The Addition of Copper Nanoparticles to Mineral Trioxide Aggregate for Improving the Physical and Antibacterial Properties

Fakhriza,

Rusdiarso,

Sunarintyas

et al. 2023

Indones. J. Chem.

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The physical and antibacterial properties of mineral trioxide aggregate (MTA) have been improved by adding copper nanoparticles (CuNP). The CuNP colloid was synthesized by reacting CuCl2·2H2O and NaBH4 as the reducing agent using C6H8O6 as the capping agent. The Cu(II) concentration was varied by 3.0, 6.0, and 9.0 mM to produce CuNP-3, CuNP-6, and CuNP-9 colloids, respectively. The CuNP colloids were characterized with a UV-Vis spectrophotometer and TEM. MTA was hydrated with CuNP at a mass-to-volume ratio of 2:1 to produce Cu-MTA-3, Cu-MTA-6, and Cu-MTA-9, respectively. All products were characterized with XRD and SEM-EDX. The compressive strength, pH, Ca ion release, and solubility were measured, and antibacterial activity was tested. The results showed a spherical shape of the synthesized CuNP with a particle size of ~28.08 nm. Adding CuNP-9 to hydrated MTA increased the compressive strength, pH, Ca ion release, and solubility, with the value of 4.78±0.38 MPa; 9.01±0.03; 1718±63 ppm, and 22.48±0.37%, respectively. The highest antibacterial activity occurred for Cu-MTA-9, with an inhibition zone of 10.15±0.47 mm against S. aureus and 11.93±1.16 mm against P. aeruginosa. The findings show a potential application of the product for endodontic materials containing antibacterial agents.

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The antibacterial activity of mineral trioxide aggregate containing calcium fluoride

Cited by 14 publications

References 35 publications

Histologic Assessment of a Fast-Set Mineral Trioxide Aggregate (MTA) and Two Novel Antibacterial-Enhanced Fast-Set MTAs for Apexification and Periapical Healing of Teeth With Incomplete Root Formation in a Rat Model: An In Vivo Animal Study

Histologic Assessment of a Fast-Set Mineral Trioxide Aggregate (MTA) and Two Novel Antibacterial-Enhanced Fast-Set MTAs for Apexification and Periapical Healing of Teeth With Incomplete Root Formation in a Rat Model: An In Vivo Animal Study

Comparative Evaluation of Push-Out Bond Strength of Conventional Mineral Trioxide Aggregate, Biodentine, a Modified Mineral Trioxide Aggregate, and Two Novel Antibacterial-Enhanced Mineral Trioxide Aggregates

The Addition of Copper Nanoparticles to Mineral Trioxide Aggregate for Improving the Physical and Antibacterial Properties

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