The antibacterial activity of photodynamic agents against multidrug resistant bacteria causing wound infection

Akbıyık, Ayşe; Taşlı, Hüseyin; Topaloğlu, Nermin; Alptüzün, Vildan; Parlar, Sülünay; Kaya, Selçuk

doi:10.1016/j.pdpdt.2022.103066

Cited by 8 publications

(6 citation statements)

References 60 publications

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“…In this study, CPDs synthesized by us as photosensitizers named PM, PE, PN, and PL were used. CPDs can absorb a wavelength ranging from 250 to 800 nm in the broad spectrum and the maximum light absorption is 422±3 nm (27). The antimicrobial activities of CPDs without light exposure are almost non-existent (26).…”

Section: Methods and Materials 31 Photosensitizers Used In Experimentsmentioning

confidence: 99%

“…However, the survival reduction of up to 57% in fibroblast cells in live cell toxicity experiments was not a desired result for the photosensitizer. The toxicity was associated with the excess concentrations used in the experiments (27). Therefore, it is predicted that strong antimicrobial activity can be obtained at lower concentrations by using blue light instead of red light.…”

Section: Introductionmentioning

confidence: 99%

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An alternative approach for daily perineal care of patients with indwelling urinary catheterization: Photodynamic inactivation with cationic porphyrin derivatives

Akbıyık,

Kaya,

Topaloğlu Avşar

et al. 2023

HumanSciences

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Background: Catheter-associated urinary tract infections (CAUTI) constitute a significant portion of healthcare-associated infections. Using antiseptic for routine daily perineal care of patients with IUC may reduce CAUTIs. Aim: This study aimed to examine antimicrobial photodynamic inactivation (aPDI) against clinical isolates for use in the daily perineal care of patients with IUC. In addition, it was also aimed to compare the antimicrobial activities of aPDI and 0.1% chlorhexidine gluconate. Methods: In this in-vitro study, cationic porphyrin derivatives (CPDs) were used as photosensitizers in the experiments. CPDs, named PM, PE, PN, and PL were synthesized by the researchers. A diode laser device emitting light with a wavelength of 450 nm (blue light) was used as the light source. Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Klebsiella pneumoniae with multidrug-resistant (MDR) properties and Candida albicans were used. Photosensitizer (PS), aPDI, light (L), and control (C) groups in aPDI experiments; control (C) and chlorhexidine gluconate 0.1% groups were used in the chlorhexidine gluconate experiments. Survival was calculated based on CFU/mL in the control group. Results: In experiments, combinations of 25 J/cm² with 6.25 and 3.125 µM PM, PE reduced E. coli, K. pneumoniae, MRSA, and C. albicans survival in the range of 8.70 to 11.53 log₁₀. In aPDI experiments performed with 6.25 and 3.125 µM PN and PL concentrations at the same energy density, reductions in the range of 4.41 to 0.17 log₁₀ were observed in all four clinical isolates. In experiments where 1.5625 µM concentration was used, survival decreased in the range of 8.29 to 10.87 log₁₀ in PM and PE, while antimicrobial activity was limited in PN and PL. In the 0.1% chlorhexidine gluconate experiments, the survival reduction in all four clinical isolates ranged from 8.87 to 10.24 log₁₀. Conclusion: For PM and PE, a very strong aPDI was obtained in C. albicans, E.coli, K. pneumoniae, and MRSA at low concentrations and energy density. The same antimicrobial activity was found in experiments using 0.1% chlorhexidine gluconate. In this context, we would like to inform you that aPDI to be performed with a combination of 25 J/cm² at 6.25 and 3.125 µM concentrations of PM and PE has the potential to be an antiseptic in the daily perineal care of patients with IUC.

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Section: Methods and Materials 31 Photosensitizers Used In Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An alternative approach for daily perineal care of patients with indwelling urinary catheterization: Photodynamic inactivation with cationic porphyrin derivatives

Akbıyık,

Kaya,

Topaloğlu Avşar

et al. 2023

HumanSciences

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“…Chronic wound and skin infections can be caused by the colonization of bacteria which will delay the process of wound healing. [158] Wound healing is an intricate process in response to the injury site to regain the appearance of the damaged skin. [159] The skin is the front line of immune defense and acts as a shield against the attack of foreign microbes.…”

Section: Chronic Wound Infections and Skin Infectionsmentioning

confidence: 99%

Antimicrobial Photodynamic Therapy for the Remote Eradication of Bacteria

Xin

Kwek

et al. 2023

ChemPlusChem

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The emergence of multi‐drug resistant bacteria strains has been an uphill battle in modern healthcare worldwide, due to the increasing difficulty of killing them. The evolving pathogenicity of bacteria has led to researchers searching for more effective antimicrobial therapeutics to successfully eliminate them without undesirable consequences to the human body. In recent years, antimicrobial photodynamic therapy (APDT), an obsolete technique for cancer treatments, has been reported to eradicate bacteria and biofilm‐related infections. The principle of antimicrobial photodynamic therapy solely relies on the photosensitizers (PSs) generating reactive oxygen species, in the presence of oxygen and light, to destroy pathogens. Thus, it can target a broad spectrum of microorganisms, owing to the indirect interaction between PSs and the bacteria, resulting in the less likelihood for the development of drug resistant bacteria strains. This review will focus on the recent progress of APDT in the last five years and some future perspectives of APDT. The mechanism of APDT against bacteria and biofilms, various PSs used for APDT, and some common multidrug‐resistant bacteria strains will be briefly introduced. The reported in vivo applications of APDT in the several types of bacterial infections that includes periodontitis, wound infections, keratitis, endophthalmitis and tuberculosis in the last five years will be summarized in detail.

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“…[12,13] UV irradiation-based therapies, known as PDT, also became an alternative to the traditional medical treatments for inactivation of viruses, fungi, mycoplasma, bacteria, and multidrug-resistant bacteria. [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] Most of the suggested PDT-based applications involve nanoparticles or nanofiber-based biomaterials loaded with photosensitizers to accelerate wound healing. [29] Despite the profound benefits of PDT applications, photosensitizers have some negative effects on human skin, due to high toxicity and inhibition of collagen regeneration due to the release of photosensitizer into the wound.…”

Section: Introductionmentioning

confidence: 99%

Accelerating Wound Healing with Combinatorial Therapy Using Natural Melanin Nanoparticle‐Polyvinyl Alcohol Blend and Core‐Sheath Hybrid Nanofibers under UV‐A Irradiation

Bayrak,

Yiğit,

Bakay

et al. 2024

Macro Materials & Eng

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Wound healing can be a complex and slow process for the human body, particularly if it is chronic. Photothermal therapy (PTT) and photodynamic therapy (PDT) can be used to accelerate wound healing. As opposed to current PDT studies using well‐known photosensitizers, this work use novel polyvinyl alcohol (PVA) and melanin nanoparticles (MNPs) containing hybrid nanofibers prepared by electrospinning techniques to enhance wound healing with/without UV light. Blend and core‐sheath nozzles are utilized to obtain sustainable and biocompatible MNP‐PVA blend, and core‐sheath hybrid nanofibers and 30, 60, and 300 s of UV‐A irradiation are tested in terms of photoinactivation efficiencies for Escherichia coli (E. coli). The MNP‐PVA blend with a diameter of 324 nm and the core‐sheath nanofibers with a diameter of 468 nm both showed killing effect on E. coli about 41.6% and 32%, respectively, under 30 s of UV‐A irradiation. Increased irradiation time activates the protective effect of MNPs located in nanofibers, thereby decreasing photoinactivation efficiency. Moreover, the MNP‐PVA core‐sheath nanofibers with 30 s of UV‐A irradiation promote the closure of wound to 99.2% at 24 h.

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The antibacterial activity of photodynamic agents against multidrug resistant bacteria causing wound infection

Cited by 8 publications

References 60 publications

An alternative approach for daily perineal care of patients with indwelling urinary catheterization: Photodynamic inactivation with cationic porphyrin derivatives

An alternative approach for daily perineal care of patients with indwelling urinary catheterization: Photodynamic inactivation with cationic porphyrin derivatives

Antimicrobial Photodynamic Therapy for the Remote Eradication of Bacteria

Accelerating Wound Healing with Combinatorial Therapy Using Natural Melanin Nanoparticle‐Polyvinyl Alcohol Blend and Core‐Sheath Hybrid Nanofibers under UV‐A Irradiation

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