The antibacterial and anti-biofouling performance of biogenic silver nanoparticles byLactobacillus fermentum

Abstract

Biofouling is a major challenge in the water industry and public health. Silver nanoparticles (AgNPs) have excellent antimicrobial properties and are considered to be a promising anti-biofouling agent. A modified method was used to produce small sized and well-dispersed biogenic silver nanoparticles with a mean size of ~6 nm (Bio-Ag0-6) using Lactobacillus fermentum. The morphology, size distribution, zeta potential and oxidation state of the silver were systematically characterized. Determination of minimal inhibitory and bactericidal concentration results revealed that biogenic silver Bio-Ag(0-6) can effectively suppress the growth of the test bacteria. Additionally, the inhibition effects of Bio-Ag(0-6) on biofilm formation and on established biofilms were evaluated using P. aeruginosa (ATCC 27853) as the model bacterium. The results from microtiter plates and confocal laser scanning microscopy demonstrated that Bio-Ag(0-6) not only exhibited excellent antibacterial performance but also could control biofilm formation and induce detachment of the bulk of P. aeruginosa biofilms leaving a small residual matrix.

Silver Nanoparticles as Potential Antibacterial Agents

“…Indeed, Pseudomonas aeruginosa and Vibrio cholera were more resistant than E. coli and Salmonella typhi, but at concentrations above 75 μg/mL, the bacterial growth was completely abolished [50]. In this perspective, Kim et al [23] studied AgNPs antimicrobial activity against E. coli and S. aureus showing that E. coli was inhibited at low concentrations, while the inhibitory effects on the growth of S. aureus were less marked [46].…”

“…Alteration of membrane [28,41,47] Listeria monocytogenes Morphological changes, separation of the cytoplasmic membrane from the cell wall, plasmolysis [47] Micrococcus luteus Alteration of membrane [28] Nitrifying bacteria inhibits respiratory activity [31] Pseudomonas aeruginosa Irreversible damage on bacterial cells; Alteration of membrane permeability and respiration [17,28,32,33,36,[41][42][43][44][48][49][50] Proteus mirabilis Alteration of cell wall and cytoplasm. [43,44] …”

Nanomedicine opened new horizons for metal nanoparticles to treat multi-drug resistant organisms

“…Indeed, Pseudomonas aeruginosa and Vibrio cholera were more resistant than E. coli and Salmonella typhi, but at concentrations above 75 μg/mL, the bacterial growth was completely abolished (50). In this perspective, Kim et al,2007(34) studied NPs antimicrobial activity against E. coli and S. aureus showing that E. coli was inhibited at low concentrations, while the inhibitory effects on the growth of S. aureus were less marked (65).…”

Understanding bioenergy production and optimisation at the nanoscale – a review

“…[13] A research finding showed that, Pseudomonas aeruginosa and Vibrio cholera were more resilient than Escherichia coli and Salmonella typhi, but at concentrations above 75 mg/mL, the bacterial growth was completely abolished. [14] In this perspective, Sweet et al [15] studied Ag NPs' antimicrobial activity against E. coli and S. aureus showing that E. coli was inhibited at low concentrations, while the inhibitory effects on the growth of S. aureus were less noticeable. [16] Silver nanoparticles have also significant adverse effects on growth and morphology of filamentous green algae.…”