The Antibacterial and Antioxidant Activity of Moringa Oleifera Seed Oil Extract Against Some Foodborne Pathogens

Abstract

Moringa oleifera is one of the most important and useful plants because it is both a medical and nutritional plant. The aim of this study is to determine the antioxidant and antibacterial activity of Moringa oleifera seed oil extract. Petroleum ether solvent in Soxhlet apparatus was used to prepare the seed oil. Many tests were conducted included, Gas Chromatography-Mass Spectrum (GC-MS), evaluation of antioxidant activity utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, total phenolic content. The oil extract of Moringa oleifera was investigated against some foodborne pathogens include Staphylococcus aureus, Bacillus cereus, Escherichia coli and Klebsiella pneumonia. The results of GC-MS revealed to the identification of twenty two components, the prevalent compounds were Oleic acid (14.61 %), Butyric acid (11.55 %), 8-Octadecenoic acid, methyl ester (11.16 %) and 1-Pentanol (10.75 %). The radical scavenging activity of the seed oil was 75.81% in concentration 100 µl/ml, and the (EC 50 ) was 25 µl \ml. Furthermore, the total phenolic content of the seed oil extract increased gradually with the increases of concentration. The results of the antibacterial activity of Moringa oleifera oil extract showed that the oil extract have the antibacterial effect against all tested bacteria in all concentrations except E. coli and K. pneumonia which were resistant to the seed's oil extract in concentration 125 µl/ml. The highest antibacterial activity was observed on Staph. aureus, B. cereus, E. coli and K. pneumonia with inhibition zone 18.50, 14.16, 11.83 and 11.33 mm in concentration 500 µl/ml respectively. Furthermore, the MIC of the oil extract was 64µl/ml for Staph. aureus, 128 µl/ml for Bacillus cereus and 256 µl/ml for each E. coli and K. pneumonia.

[Retracted] Comparative Analysis of Antioxidants Activity of Indigenously Produced Moringa Oleifera Seeds Extracts

“…The seeds of the plant exhibit various biological activities like, antidiabetic [ 31 ], anti-inflammatory [ 32 ], hepatoprotective [ 33 ], anticancer [ 34 ], cardiovascular, CNS, analgesic [ 35 ], wound healing [ 36 ], antiallergic, antifertility, antiasthmatic [ 37 ], antiulcer, and antipyretic activity [ 38 ]. Therefore, this study was designed to evaluate the antioxidant potential of M. oleifera seeds of two different accessions from different regions.…”