The antibacterial and toxicological studies of mycosynthesis silver nanoparticles by isolated phenols from agaricus bisporus

Abstract

Silver nanoparticles (AgNPs) are widely utilized in biomedical and antibacterial fields. However, their use poses a risk and danger of Ag+ absorption into human tissue via skin penetration. This study focuses on the facile and eco-friendly approach to synthesizing uniformly distributed and spherically shaped phenol capped AgNPs using Agaricus bisporus. The synthesis process was monitored by UV–vis spectroscopy peak at 430 nm. Further characterizations were performed by XRD, and TEM. The phenol-capped AgNPs (average diameter is 11.81 nm) exhibit excellent colloidal stability in an aqueous solution with a zeta potential of −32.9 mV. The presence of phenols in the AgNPs suspension, which include CAFFICACID, QUERCETIN, GALLIC ACID, ROSMARINIC ACID, SINAPIC, and SYNIRGIC was confirmed by HPLC analysis. The study investigated their antibacterial activity against colonies of palm-hand bacteria, Pseudomonas aeruginosa, and Streptococcus pyogenes bacteria even in small doses was investigated. Damage to the cell wall, mitochondria, and DNA by nanoparticles has been proposed as an antibacterial mechanism. Toxicological parameters such as histological study of skin and vital organ tissue, hematological, serum biochemistry, and fatality incidence were investigated. There were no harmful effects or damage caused by phenol-capped AgNPs in albino mouse organs when compared to the vehicle group. Toxicological evidence supports the use of mycosynthesized phenol-capped AgNPs in non-alcohol hand sanitizer.