The Antibacterial Assay of Tectorigenin with Detergents or ATPase Inhibitors against Methicillin‐Resistant <i>Staphylococcus aureus</i>

Joung, Dae-Ki; Mun, Su‐Hyun; Lee, Kuang-Shim; Kang, Ok‐Hwa; Choi, Jang‐Gi; Kim, Sung‐Bae; Gong, Ryong; Chong, Myong-Soo; Kim, Youn‐Chul; Lee, Dong-Sung; Shin, Dong‐Won; Kwon, Dong‐Yeul

doi:10.1155/2014/716509

Cited by 16 publications

(21 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Understanding the fine ultrastructure of the bacterial cell wall is important to gain insight into bacterial physiology and the mechanism of action of antibiotics against bacteria (40). Using TEM to observe morphological changes in bacterial cells provides useful insights into the mechanism underlying the activity of antibacterial agents (41). When bacteria cells were treated with URS and OXA, cell membrane disintegration, cell lysis, and release of cytoplasmic contents were observed (Fig.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity and synergism of ursolic acid 3-O-α-L-arabinopyranoside with oxacillin against methicillin-resistant Staphylococcus aureus

Zhou

Kang

et al. 2017

International Journal of Molecular Medicine

Self Cite

View full text Add to dashboard Cite

The objective of the present study was to investigate the antibacterial activity of a single constituent, ursolic acid 3-O-α-L-arabinopyranoside (URS), isolated from the leaves of Acanthopanax henryi (Oliv.) Harms, alone and in combination with oxacillin (OXA) against methicillin-resistant Staphylococcus aureus (MRSA). A broth microdilution assay was used to determine the minimal inhibitory concentration (MIC). The synergistic effects of URS and OXA were determined using a checkerboard dilution test and time-kill curve assay. The mechanism of action of URS against MRSA was analyzed using a viability assay in the presence of a detergent and an ATPase inhibitor. Morphological changes in the URS-treated MRSA strains were evaluated via transmission electron microscopy (TEM). In addition, the producing penicillin-binding protein 2a (PBP2a) protein level was analyzed using western blotting. The MIC value of URS against MRSA was found to be 6.25 µg/ml and there was a partial synergistic effect between OXA and URS. The time-kill growth curves were suppressed by OXA combined with URS at a sub-inhibitory level. Compared to the optical density at 600 nm (OD600) value of URS alone (0.09 µg/ml), the OD600 values of the suspension in the presence of 0.09 µg/ml URS and 0.00001% Triton X-100 or 250 µg/ml N,N'-dicyclohexylcarbodiimide reduced by 56.6 and 85.9%, respectively. The TEM images of MRSA indicated damage to the cell wall, broken cell membranes and cell lysis following treatment with URS and OXA. Finally, an inhibitory effect on the expression of PBP2a protein was observed when cells were treated with URS and OXA compared with untreated controls. The present study suggested that URS was significantly active against MRSA infections and revealed the potential of URS as an effective natural antibiotic.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity and synergism of ursolic acid 3-O-α-L-arabinopyranoside with oxacillin against methicillin-resistant Staphylococcus aureus

Zhou

Kang

et al. 2017

International Journal of Molecular Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…DCCD disturbs the H + -translocating sector (F 0 ) of the F 0 F 1 -ATP synthase in coupling membranes [ 32 ]. Most bacteria produce ABC transporters, which comprise an essential uptake system for amino acids in bacteria and are shown to be involved in multidrug resistance including that of MRSA [ 23 , 31 ]. NaN 3 is known to inhibit ATP synthesis in mitochondria and ATP-dependent transport systems such as endocytosis by disrupting electrochemical proton gradients in bacterial cells [ 20 , 24 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…To determine whether SKN directly bound PGN in S. aureus cell wall, SKN (7.8 μ g/mL, 0.5 MIC) was added together with PGN (1.9–125 μ g/mL) to MRSA cultures as previously described [ 23 ]. Lipopolysaccharide (LPS) was used as negative control; it did not demonstrate inhibitory activity against MRSA.…”

Section: Methodsmentioning

confidence: 99%

“…When MRSA cultures reached the midlogarithmic phase (approximately 1.5 × 10 6 colony-forming units [CFU]/mL), they were treated with 7.8 μ g/mL and 15.6 μ g/mL of SKN for 10 h. Then, 2 mL of the cultures was centrifuged at 10,000 ×g for 10 min, and the pellets were treated with Karnovsky's fixative and examined under an energy-filtering transmission electron microscope (LIBRA 120; Carl Zeiss, Oberkochen, Germany) at an accelerating voltage of 100 kV. Images were obtained using a 4 k × 4 k slow-scan charge-coupled device camera (Ultrascan 4000 SP; Gatan, Pleasanton, CA, USA) attached to the microscope [ 23 , 24 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-ResistantStaphylococcus aureus

Lee

Kim

et al. 2015

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Shikonin (SKN), a highly liposoluble naphthoquinone pigment isolated from the roots of Lithospermum erythrorhizon, is known to exert antibacterial, wound-healing, anti-inflammatory, antithrombotic, and antitumor effects. The aim of this study was to examine SKN antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). The SKN was analyzed in combination with membrane-permeabilizing agents Tris and Triton X-100, ATPase inhibitors sodium azide and N,N′-dicyclohexylcarbodiimide, and S. aureus-derived peptidoglycan; the effects on MRSA viability were evaluated by the broth microdilution method, time-kill test, and transmission electron microscopy. Addition of membrane-permeabilizing agents or ATPase inhibitors together with a low dose of SKN potentiated SKN anti-MRSA activity, as evidenced by the reduction of MRSA cell density by 75% compared to that observed when SKN was used alone; in contrast, addition of peptidoglycan blocked the antibacterial activity of SKN. The results indicate that the anti-MRSA effect of SKN is associated with its affinity to peptidoglycan, the permeability of the cytoplasmic membrane, and the activity of ATP-binding cassette (ABC) transporters. This study revealed the potential of SKN as an effective natural antibiotic and of its possible use to substantially reduce the use of existing antibiotic may also be important for understanding the mechanism underlying the antibacterial activity of natural compounds.

show abstract

“…Tec, which inhibits the inflammation of acute lung injury in mice, is derived from the Chinese herb Belamcanda chinensis ( Ma et al, 2014 ). Another study has shown Tec antibacterial activity against methicillin-resistant Staphylococcus aureus ( Joung et al, 2014 ). Therefore, Tec may represent a potentially effective option to treat inflammation.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory and anti-osteoarthritis effects of tectorigenin

Wang

et al. 2017

Biology Open

View full text Add to dashboard Cite

Osteoarthritis (OA) is a common and dynamic disease of the joints, including the articular cartilage, underlying bones and synovium. In particular, OA is considered as the degeneration of the cartilage. Tectorigenin (Tec) is known to affect many biological processes; however, its effects on articular chondrocytes remain unclear. This study aimed to assess the effects of Tec on articular cartilage. In vitro, Tec inhibited the expression levels of type X collagen, cyclooxigenase-2, matrix metalloproteinase (MMP)-3 and MMP-13, but enhanced the expression of Runx1, type II collagen and aggrecan in the presence of IL-1β. Meanwhile, Tec inhibited apoptosis through the Bax/Bcl-2/caspase-3 pathway, upregulating p-Bad, downregulating the Bax/Bcl-2 ratio, and activating caspase-3 compared with IL-1β treatment only. Moreover, this process was partially regulated by NF-κB P65. In vivo, the chondroprotective effects of Tec were assessed by establishing a model of surgically induced OA. Tec-treated joints exhibited fewer osteoarthritic changes than saline-treated joints. Meanwhile, 1.5 μg/kg Tec treatment produced a greater protective effect than 0.75 μg/kg Tec. The Osteoarthritis Research Society International (OARSI) scoring system, employed to assess histopathological grading of the models, as well immunohistochemistry for Aggrecan Neoepitope and MMP-3, further confirmed the results. In conclusion, this study showed that Tec plays a chondroprotective role in the OA process by preventing articular cartilage degeneration and chondrocyte apoptosis via the NF-κB P65 pathway.

show abstract

The Antibacterial Assay of Tectorigenin with Detergents or ATPase Inhibitors against Methicillin‐Resistant Staphylococcus aureus

Cited by 16 publications

References 34 publications

Antimicrobial activity and synergism of ursolic acid 3-O-α-L-arabinopyranoside with oxacillin against methicillin-resistant Staphylococcus aureus

Antimicrobial activity and synergism of ursolic acid 3-O-α-L-arabinopyranoside with oxacillin against methicillin-resistant Staphylococcus aureus

The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-ResistantStaphylococcus aureus

Anti-inflammatory and anti-osteoarthritis effects of tectorigenin

Contact Info

Product

Resources

About