The antibacterial effect of nitric oxide against ESBL-producing uropathogenic E. coli is improved by combination with miconazole and polymyxin B nonapeptide

Bang, Charlotte Sahlberg; Kinnunen, Annica; Karlsson, Marie; Önnberg, Anna; Söderquist, Bo; Persson, Katarina

doi:10.1186/1471-2180-14-65

Cited by 27 publications

(14 citation statements)

References 41 publications

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“…It has already been shown that NO can have an antimicrobial effect against multidrug-resistant uropathogenic E. coli . [44] In addition, a manganese nitrosyl, entrapped in the mesoporous material MCM-41 has been discovered to strongly inhibit the growth of drug-resistant A. baumannii . [45] Nevertheless, most of these studies to date have focused on the bactericidal properties of NO on planktonic cells.…”

Section: Resultsmentioning

confidence: 99%

Thromboresistant/anti-biofilm catheters via electrochemically modulated nitric oxide release

Ren

Colletta

Koley

et al. 2015

Bioelectrochemistry

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Inexpensive nitric oxide (NO) release strategies to prevent thrombosis and bacterial infections are desirable for implantable medical devices. Herein, we demonstrate the utility of electrochemically modulated NO release from a catheter model using an inner copper wire working electrode and an inorganic nitrite salt solution reservoir. These catheters generate NO surface fluxes of >1.0×10−10 mol min−1 cm−2 for more than 60 h. Catheters with an NO flux of 1.1×10−10 mol min−1 cm−2 are shown to significantly reduce surface thrombus formation when implanted in rabbit veins for 7 h. Further, the ability of these catheters to exhibit anti-biofilm properties against bacterial species commonly causing bloodstream and urinary catheter infections is examined. Catheters releasing NO continuously during the 2 d growth of S. aureus exhibit a 6 log-unit reduction in viable surface bacteria. We also demonstrate that catheters generating NO for only 3 h at a flux of 1.0×10−10 mol min−1 cm−2 lower the live bacterial counts of both 2 d and 4 d pre-formed E. coli biofilms by >99.9%. Overall, the new electrochemical NO-release devices could provide a cost-effective strategy to greatly enhance the biocompatibility and antimicrobial properties of intravascular and urinary catheters, as well as other implantable medical devices.

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Section: Resultsmentioning

confidence: 99%

Thromboresistant/anti-biofilm catheters via electrochemically modulated nitric oxide release

Ren

Colletta

Koley

et al. 2015

Bioelectrochemistry

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“…5,25 More recent data correlated the antibacterial activity with the inhibition of bacterial flavohaemoglobins. 26,27 Especially in inflammatory skin disease, such as atopic dermatitis, seborrhoeic dermatitis and psoriasis, microbes are believed to trigger, exacerbate or sustain the pathological processes. 3,4,6 Azoles with their combined antifungal and antibacterial effects against staphylococci, streptococci, dermatophytes and yeasts can be of great use in dermatology where infections are often mixed.…”

Section: Discussionmentioning

confidence: 99%

New insights on the antibacterial efficacy of miconazole in vitro

Nenoff

Koch

Krüger

et al. 2017

Mycoses

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SummaryMiconazole is a broad-spectrum antifungal used in topical preparations. In the present investigation the minimal inhibitory concentration (MIC) of miconazole for eighty wild type strains of gram-positive and gram-negative bacteria isolated from infected skin lesions was assessed using a modified agar dilution test (adapted to CLSI, Clinical Laboratory Standards Institute). 14 ATCC reference strains served as controls.Miconazole was found efficacious against gram-positive aerobic bacteria (n=62 species), the MICs against Staphylococcus (S.) aureus, S. spp., Streptococcus spp. und Enterococcus spp. ranged between 0.78 and 6.25 μg/mL. Interestingly, there were no differences in susceptibility between methicillin-susceptible (MSSA, 3) methicillin- resistant (MRSA, 6) and fusidic acid-resistant (FRSA, 2) S. aureus isolates. Strains ofStreptococcus pyogenes (A-streptococci) (8) were found to be slightly more sensitive (0.78-1.563 μg/mL), while for gram-negative bacteria, no efficacy was found within the concentrations tested (MIC >200 μg/mL). In conclusion, for the gram-positive aerobic bacteria the MICs of miconazole were found within a range which is much lower than the concentration of miconazole used in topical preparations (2%). Thus topically applied miconazole might be a therapeutic option in skin infections especially caused by gram-positive bacteria even by those strains which are resistant to antibiotics.

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“…An extensive literature exists on the NO detoxification mechanisms of E. coli and is dominated by articles on the flavohemoglobin Hmp, reflecting the detoxification of NO to nitrate as an important process during infection. Indeed, the UPEC strain J96 has previously been shown to out-compete an isogenic hmp mutant in bladder and kidney colonisation in the mouse UTI model 14 , and pharmacological modulation of Hmp activity has recently been shown to elevate NO sensitivity in multidrug-resistant UPEC strains 34 . It is therefore reasonable to assume that Hmp provides considerable protection against nitrosative stress in vivo .…”

Section: Discussionmentioning

confidence: 99%

The cytochrome bd-I respiratory oxidase augments survival of multidrug-resistant Escherichia coli during infection

Shepherd

Achard

Idris

et al. 2016

Sci Rep

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Nitric oxide (NO) is a toxic free radical produced by neutrophils and macrophages in response to infection. Uropathogenic Escherichia coli (UPEC) induces a variety of defence mechanisms in response to NO, including direct NO detoxification (Hmp, NorVW, NrfA), iron-sulphur cluster repair (YtfE), and the expression of the NO-tolerant cytochrome bd-I respiratory oxidase (CydAB). The current study quantifies the relative contribution of these systems to UPEC growth and survival during infection. Loss of the flavohemoglobin Hmp and cytochrome bd-I elicit the greatest sensitivity to NO-mediated growth inhibition, whereas all but the periplasmic nitrite reductase NrfA provide protection against neutrophil killing and promote survival within activated macrophages. Intriguingly, the cytochrome bd-I respiratory oxidase was the only system that augmented UPEC survival in a mouse model after 2 days, suggesting that maintaining aerobic respiration under conditions of nitrosative stress is a key factor for host colonisation. These findings suggest that while UPEC have acquired a host of specialized mechanisms to evade nitrosative stresses, the cytochrome bd-I respiratory oxidase is the main contributor to NO tolerance and host colonisation under microaerobic conditions. This respiratory complex is therefore of major importance for the accumulation of high bacterial loads during infection of the urinary tract.

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The antibacterial effect of nitric oxide against ESBL-producing uropathogenic E. coli is improved by combination with miconazole and polymyxin B nonapeptide

Cited by 27 publications

References 41 publications

Thromboresistant/anti-biofilm catheters via electrochemically modulated nitric oxide release

Thromboresistant/anti-biofilm catheters via electrochemically modulated nitric oxide release

New insights on the antibacterial efficacy of miconazole in vitro

The cytochrome bd-I respiratory oxidase augments survival of multidrug-resistant Escherichia coli during infection

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