The Antibacterial Effects of Apacaries Gel on Streptococcus mutans: An in vitro Study

Abstract

Background: New approaches for chemomechanical caries removal require effective materials with antibacterial properties for removal of infected dentin. Apacaries gel is a newly developed material comprised polyphenol from mangosteen extracts and papain mixed in gel preparation.Aim: This study evaluated the antibacterial effects of Apacaries gel on Streptococcus mutans in vitro.Materials and methods: Mangosteen pericarp powder was extracted. The amount of phenolic compounds was determined using the Folin-Ciocalteu method. The time-kill kinetics were investigated. Mangosteen extract and papain were mixed with gel base to develop Apacaries gel. The inhibition zone of the Apacaries gel was determined using agar well diffusion methods.Results: The mangosteen pericarp extract, which contains α-mangostin, was active against S. mutans strain ATCC25175. The time-kill kinetics curve showed that applying 1 mg/ml of mangosteen extract can reduce S. mutans by 50% within approximately 5 seconds; after this reduction, the bacterial count rapidly dropped to 0 within 60 seconds. Using mangosteen extract and papain mixture gel preparation resulted in a larger inhibition zone than using the mangosteen extract gel or papain gel separately.Conclusion: Apacaries gel can effectively inhibit S. mutans strain ATCC25175. Apacaries is capable of S. mutans inhibition better than both mangosteen extract or papain separately.How to cite this article: Juntavee A, Peerapattana J, Ratanathongkam A, Nualkaew N, Chatchiwiwattana S, Treesuwan P.The Antibacterial Effects of Apacaries Gel on Streptococcus mutans: An in vitro Study. Int J Clin Pediatr Dent 2014;7(2):77-81.

Polyphenols in Dental Applications

“…Apacaries gel is a material containing polyphenols from mangosteen extracts and papain as an enzyme [104]. This gel, including polyphenolic compounds, such as gallic acid, which have antibacterial effects, could play an interesting role in the removal of various tissues.…”

Alternative Caries Removal Methods

“…36 Apacarie jel ise daha önce de belirtildiği gibi mangosten ekstractlarından elde edilen polifenol ve papain jel karışımı olup antibakteriyel etkisi üzerine yoğunlaşılmaktadır. 47 F Fo ot to o--a ab bl la as sy yo on n Y Yö ön nt te em ml le er ri i: : Günümüzde çürü-ğün uzaklaştırılmasında lazer uygulanması da ayrıca büyük ilgi görmektedir. Kavite preparasyonunda kullanılmak üzere birçok lazer araştırıl-mış; en efektif olarak erbium bazlı (Er:YAG ve Er,Cr:YSGG) lazerler bulunmuştur.…”

Development of a new Colombian product, effective for the treatment of carious lesions: a case study

“…The α1-anti-trypsin inhibited the digestion of proteins, therefore, as the infected tissue does not present α1-antitrypsin, papain acts by "breaking" the collagen molecules partially degraded by the action of caries, since the It has the capacity to digest dead cells [15].…”