The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): Variation in laboratory evaluation

Barnes, Kate M.; Dixon, Ronald; Gennard, Dorothy

doi:10.1016/j.mimet.2010.06.005

Cited by 47 publications

(20 citation statements)

References 16 publications

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“…Although several studies focused on MDT, a very limited data have been obtained until now. These researches are mainly based on ES and WBE of sterile and/or patient-applied maggots [24,[32][33][34][35][36][37][38]. In this study, maggots are collected from laboratory conditions and differently from "liver culture" to create a counterfeit environment that maggots suppose like they are on an infected wound.…”

Section: Discussionmentioning

confidence: 99%

Investigation of whole body extract metabolites of Lucilia sericata larvae and potential antibacterial effects

Sığ

Koru

Araz

2018

Turkish Journal of Clinics and Laboratory

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Aim: Complementary medicinal techniques have gained focus by modern medicine, recently. Maggot Debridement Therapy is a widely-used method worldwide. It is especially recommended for chronic wounds, and has serious advantages such as low cost, easily-applicability and rare adverse effects, but its effect mechanisms remains unclear. The aim of this study is to detect components and to investigate potential antibacterial effects of whole body extract metabolites of Lucilia sericata larvae. Material anf Methods:Due to potential antibacterial effects, agar well diffusion and flowcytometry methods were used against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis to do evaluation on whole body extracts of previously-cloned maggots in specialized climate room. After this antibacterial effect evaluation, 2-D PAGE analysis was done for protein investigation.Results: Inhibition zones were observed for S.aureus (16mm), E.coli (22mm) and E.faecalis (14mm), but for P.aeruginosa, the extract could not provide any inhibiton zone. In flow cytometry, different killing rates were detected in different extract dilutions, and for the lowest (1/64) dilution, killing rates were 51.9%, 75%, 80% and 98.7% for P.aeruginosa, E.faecalis, E.coli and S.aureus, respectively. 2-D PAGE showed various proteins with different molercular mass (<10-260kDa) and pI (3-9).Conclusion: Antibacterial effects of maggot whole body extracts on tested strains are obviously detected. Many protein spots with widely variable molecular mass and isoelectric points were observed. As a result, this antibacterial effects may be caused by these proteins, but it is necessary that these proteins must be further evaluated via mass spectrometry and protein databases. To cite this article: Sig AK, Koru O, Araz E. Investigation of whole body extract metabolites of Lucilia sericata larvae and potential antibacterial effects. Turk J Clin Lab 2018; 9(3): 191-198.ÖZ Amaç: Yakın dönemde, tamamlayıcı tıp uygulamaları modern tıbbın ilgi alanına girmiştir. Maggot Debritman Tedavisi dünya çapında yaygın olarak kullanılan bir yöntemdir. Bu yöntem, özellikle kronik yaraların tedavisinde tavsiye edilmektedir ve düşük maliyet, kolay uygulanabilirlik ve nadir yan etkiler gibi avantajları bulunmaktadır, ancak yöntemin etki mekanizması henüz tam olarak ortaya konulamamıştır. Bu çalışmanın amacı, Lucilia sericata larvalarının tüm vücut ekstraktının metabolitlerini ortaya koymak ve bunların potensiyel antibakteriyel niteliğini araştırmaktır. Gereç ve Yöntemler: Antibakteriyel etkinliği araştırmak için, önceden özel iklim odalarında üretilmiş larvalarının tüm vücut ekstraktları, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia colive Enterococcus faecalisbakterileri için agar difüzyon ve akan hücreölçer ile test edilmiştir. Antibakteriyel incelemeyi takiben, iki boyutlu elektroforez ile protein araştırılması yapılmıştır. Bulgular: S.aureus (16mm), E.coli (22mm) ve E.faecalis (14mm) için inhibisyon alanı gözlenmiş ancak P.aeruginosa için ala...

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Section: Discussionmentioning

confidence: 99%

Investigation of whole body extract metabolites of Lucilia sericata larvae and potential antibacterial effects

Sığ

Koru

Araz

2018

Turkish Journal of Clinics and Laboratory

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“…Recent studies by other investigators support this possibility [ 25 ] and form the basis for ongoing characterization of the plant’s bioactive properties by authors of the present report. The capacity of bioactive components of BM to mitigate ischemia/reperfusion-associated damage to cardiac tissue has been previously demonstrated in studies revealing that polysaccharides produced by the plant, inhibit cerebral ischemia/reperfusion injury [ 26 , 27 ]. Within the present study, evaluation of the plant’s ability to mitigate ischemia-reperfusion injury was conducted in experiments demonstrating bitter melon extract effects on ischemia/reperfusion-associated infarct size magnitude.…”

Section: Discussionmentioning

confidence: 99%

Effects of Momordica charantia (Bitter Melon) on Ischemic Diabetic Myocardium

et al. 2017

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Objective: A rat model is here used to test a hypothesis that Momordica charantia (Bitter melon (BM)) extract favorably alters processes in cardiovascular tissue and is systemically relevant to the pathophysiology of type 2 diabetes (T2DM) and related cardiovascular disease. Methods: Male Lean and Zucker Obese (ZO) rats were gavage-treated for six weeks with 400 mg/kg body weight bitter melon (BM) extract suspended in mucin–water vehicle, or with vehicle (Control). Animals were segregated into four treatment groups, 10 animals in each group, according to strain (Lean or ZO) and treatment (Control or BM). Following six-week treatment periods, peripheral blood was collected from selected animals, followed by sacrifice, thoracotomy and mounting of isolated working heart setup. Results: Body mass of both Lean and ZO rats was unaffected by treatment, likewise, peripheral blood fasting glucose levels showed no significant treatment-related effects. However, some BM treatment-related improvement was noted in postischemic cardiac functions when Lean, BM-treated animals were compared to vehicle treated Lean control rats. Treatment of Lean, but not ZO, rats significantly reduced the magnitude of infarcted zone in isolated hearts subjected to 30 min of ischemia followed by 2 h of working mode reperfusion. Immunohistochemical demonstration of caspase-3 expression by isolated heart tissues subjected to 30 min of ischemia followed by 2 h of reperfusion, revealed significant correlation between BM treatment and reduced expression of this enzyme in hearts obtained from both Lean and ZO animals. The hierarchy and order of caspase-3 expression from highest to lowest was as follows: ZO rats receiving vehicle > ZO rats receiving BM extract > Lean rats treated receiving vehicle > Lean rats administered BM extract. Outcomes of analyses of peripheral blood content of cardiac-related analytics: with particular relevance to clinical application was a significant elevation in blood of ZO and ZO BM-treated, versus Lean rats of total cholesterol (high density lipoprotein HDL-c + low density lipoprotein LDL-c), with an inferred increase in HDL-c/LDL-c ratio—an outcome associated with decreased risk of atherosclerotic disease. Conclusions: BM extract failed to positively affect T2DM- and cardiovascular-related outcomes at a level suggesting use as a standalone treatment. Nevertheless, the encouraging effects of BM in enhancement of cardiac function, suppression of post-ischemic/reperfused infarct size extent and capacity to modulate serum cholesterol, will likely make it useful as an adjuvant therapy for the management of T2DM and related cardiovascular diseases.

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“…The efÞcacy of Lucilia ES depends on the type of bacteria, the concentration of antimicrobial compounds in the ES (that is independent of bacterial concentration), and the level of bacterial stress (Barnes et al 2010). The majority of studies indicate that L. sericata ES are effective at killing gram-positive organisms, but not gram-negative (BexÞeld et al 2004(BexÞeld et al , 2008(BexÞeld et al , 2010Steenvoorde and Jukema 2004;Andersen et al 2010b).…”

Section: Insect Defense Against Pathogenic Bacteria Deleterious Bactmentioning

confidence: 99%

Bacterial Interactions with Necrophagous Flies

Thompson

Brogan

Scheifele

et al. 2013

Annals of the Entomological Society of America

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Animal remains represent ephemeral resources that provide nutrients to a wide range of organisms. On death, vertebrate carrion is immediately colonized with a variety of microorganisms (typically obligate or facultatively anaerobic bacteria from the air, from insects, or from the corpse itself), which produce odors through the breakdown of tissues, the alteration of volatile chemicals present in the environment, or both. Within minutes, certain necrophagous flies are attracted by these chemical signals, resulting in waves of oviposition and larviposition activity. Although there are certainly detrimental (pathogenic) bacteria in the milieu, there is significant evidence suggesting that the presence of bacteria in or on the corpse seems to aid in larval development and pupariation. This may be because of a change in larval nutrition, with the bacteria either being used as a food source themselves or making nutrients more available to larvae. Maggots also produce and secrete or excrete antimicrobial molecules that are effective in killing certain bacteria. It is unclear whether this is a defensive mechanism, a selective measure to enhance the survival of bacteria beneficial to the larva, or a combination of both. Significant research is still needed to fully appreciate the potential role that these bacteria—insect interactions have in conferring a competitive advantage for surviving in a carrion community.

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The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): Variation in laboratory evaluation

Cited by 47 publications

References 16 publications

Investigation of whole body extract metabolites of Lucilia sericata larvae and potential antibacterial effects

Investigation of whole body extract metabolites of Lucilia sericata larvae and potential antibacterial effects

Effects of Momordica charantia (Bitter Melon) on Ischemic Diabetic Myocardium

Bacterial Interactions with Necrophagous Flies

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