The antibiotic crisis: How bacterial predators can help

Pérez, Juana; Contreras-Moreno, Francisco Javier; Marcos‐Torres, Francisco Javier; Moraleda‐Muñoz, Aurelio; Muñoz‐Dorado, José

doi:10.1016/j.csbj.2020.09.010

Cited by 56 publications

(35 citation statements)

References 178 publications

(238 reference statements)

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“…Moreover, from 2001 to 2009 the number of hospitalizations due to S. aureus skin infections increased by 123% annually, from 160,811 to 358,212 [ 11 ]. Considering the intrinsic resistance of biofilms to antibiotic therapy, the discovery of innovative approaches able to address not only planktonic bacterial cells, but also specific features of the sessile life form, is highly pressing [ 12 ]. In this context, gene-encoded antimicrobial peptides (AMPs) of the innate immunity hold promise as novel anti-biofilm agents.…”

Section: Introductionmentioning

confidence: 99%

The Antimicrobial Peptide Temporin G: Anti-Biofilm, Anti-Persister Activities, and Potentiator Effect of Tobramycin Efficacy Against Staphylococcus aureus

Casciaro

Loffredo

Cappiello

et al. 2020

IJMS

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Bacterial biofilms are a serious threat for human health, and the Gram-positive bacterium Staphylococcus aureus is one of the microorganisms that can easily switch from a planktonic to a sessile lifestyle, providing protection from a large variety of adverse environmental conditions. Dormant non-dividing cells with low metabolic activity, named persisters, are tolerant to antibiotic treatment and are the principal cause of recalcitrant and resistant infections, including skin infections. Antimicrobial peptides (AMPs) hold promise as new anti-infective agents to treat such infections. Here for the first time, we investigated the activity of the frog-skin AMP temporin G (TG) against preformed S. aureus biofilm including persisters, as well as its efficacy in combination with tobramycin, in inhibiting S. aureus growth. TG was found to provoke ~50 to 100% reduction of biofilm viability in the concentration range from 12.5 to 100 µM vs ATCC and clinical isolates and to be active against persister cells (about 70–80% killing at 50–100 µM). Notably, sub-inhibitory concentrations of TG in combination with tobramycin were able to significantly reduce S. aureus growth, potentiating the antibiotic power. No critical cytotoxicity was detected when TG was tested in vitro up to 100 µM against human keratinocytes, confirming its safety profile for the development of a new potential anti-infective drug, especially for treatment of bacterial skin infections.

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Section: Introductionmentioning

confidence: 99%

The Antimicrobial Peptide Temporin G: Anti-Biofilm, Anti-Persister Activities, and Potentiator Effect of Tobramycin Efficacy Against Staphylococcus aureus

Casciaro

Loffredo

Cappiello

et al. 2020

IJMS

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show abstract

“…OMVs play important roles in the interactions of the microbiota, including interspecies competition. This innate antibiotic property has inspired some groups to propose the use of native OMVs as natural antibiotics ( 65 ). These “predatory OMVs” have been observed in many different systems, showing a conservation of this trait across bacterial species.…”

Section: Natural Antibiotic Properties Of Omvsmentioning

confidence: 99%

Bacterial Outer Membrane Vesicles as Antibiotic Delivery Vehicles

Collins

Brown

2021

Front. Immunol.

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Bacterial outer membrane vesicles (OMVs) are nanometer-scale, spherical vehicles released by Gram-negative bacteria into their surroundings throughout growth. These OMVs have been demonstrated to play key roles in pathogenesis by delivering certain biomolecules to host cells, including toxins and other virulence factors. In addition, this biomolecular delivery function enables OMVs to facilitate intra-bacterial communication processes, such as quorum sensing and horizontal gene transfer. The unique ability of OMVs to deliver large biomolecules across the complex Gram-negative cell envelope has inspired the use of OMVs as antibiotic delivery vehicles to overcome transport limitations. In this review, we describe the advantages, applications, and biotechnological challenges of using OMVs as antibiotic delivery vehicles, studying both natural and engineered antibiotic applications of OMVs. We argue that OMVs hold great promise as antibiotic delivery vehicles, an urgently needed application to combat the growing threat of antibiotic resistance.

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“…We are all at risk and we face a silent, slow-growing global threat that kills around 700,000 people worldwide each year due to the uncontrolled rise of superbugs resistant to antibiotics. This threat is on the rise and 10 million people will die every year due to antimicrobial-resistant infections by 2050 unless a substantial global response to the problem of AMR is executed [ 17 , 18 , 19 , 20 ]. Antibiotic resistance occurs when bacteria are not killed or their growth is not arrested by these drugs that were previously effective.…”

Section: Impact Of Antimicrobial Resistancementioning

confidence: 99%

Antibiotics Use in Food Animal Production: Escalation of Antimicrobial Resistance: Where Are We Now in Combating AMR?

Kasimanickam

2021

Medical Sciences

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The use of antibiotics has been very beneficial to human health, animal wellbeing, and food production, however, there are no alternatives to antimicrobials in treating infectious diseases. Their use can contribute to the development of antimicrobial resistance, but the world has realized the need to combat antimicrobial resistance in recent decades due to the continued escalation of the problem jeopardizing human and veterinary medicine and food and environmental safety. Understanding the AMR and judicious use of antimicrobials are critical, and one health approach involving several sectors and multiple disciplines is important to tackle the problem. National, regional, and global action plans have been instigated to tackle the escalation of AMR. Antimicrobials are frequently used in food animal production. Therefore, food animal producers are important participants to prevent overuse and misuse of antimicrobials. Recent regulations to address the challenges have not been perceived well in animal farming communities. More awareness regarding these action plans and understanding the impact of AMR are needed. A nationwide survey of perceptions of food animal producers regarding AMR mitigation approaches should be conducted to evaluate the effectiveness of the current policies regarding antibiotics use and AMR. These outcomes should be incorporated in future policies and awareness campaigns targeting food animal producers.

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The antibiotic crisis: How bacterial predators can help

Abstract: Graphical abstract

Cited by 56 publications

References 178 publications

The Antimicrobial Peptide Temporin G: Anti-Biofilm, Anti-Persister Activities, and Potentiator Effect of Tobramycin Efficacy Against Staphylococcus aureus

The Antimicrobial Peptide Temporin G: Anti-Biofilm, Anti-Persister Activities, and Potentiator Effect of Tobramycin Efficacy Against Staphylococcus aureus

Bacterial Outer Membrane Vesicles as Antibiotic Delivery Vehicles

Antibiotics Use in Food Animal Production: Escalation of Antimicrobial Resistance: Where Are We Now in Combating AMR?

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