The antibody response to specific immune complexes is under genetic control and correlates with the expression of a recurrent idiotype.

Abstract

The primary antigen-specific antibody response of various strains of mice to TEPC-15/PnC immune complexes has been examined. We found that both BALB/c and C3H mice were good responders to the PnC antigen; however, C3H mice were low responders, whereas BALB/c mice were high responders to the TEPC-15/PnC complexes. Using congenic strains on the C3H and BALB/c background, we have shown that the response to the complexes is not restricted by gene products of the H-2 complex or by the Igh (allotype) locus. However, responsiveness may be controlled by genes linked to the Igh locus, since we have shown that strains that are Ighj, Ighd, and Ighf are low responders, whereas strains that are Igha, Ighb, and Ighe are high responders to the immune complex. Moreover, responsiveness correlates with the expression of the T15 Id as measured using the anti-T15 monoclonal antibody, AB1-2. Thus, strains such as BALB/c, BALB.B, BALB.K, and CB-20, which express high levels of T15 (AB1-2) Id in their PFC response to PnC are relatively high responders to TEPC-15/PnC complexes, whereas C3H, C3H.SW, and C3H-OH, which express low levels of the T15 (AB1-2) Id, are low responders to the complexes. Finally, we found that BALB/c mice are high responders to complexes formed with T15+ antibodies, whereas they are low responders to complexes formed using T15- antibodies. The results suggest that the antigen-specific response to these immune complexes is Id-restricted.

Protective antibodies against a sphingomyelinase D from Loxosceles intermedia spider venom elicited in mice with different genetic background

“…In C57 mice, which is considering low responder HLA [35] we observed immunoglobulin secretion, but their sera was not as good as BC (high responder) sera in preventing the effects of rLiD1 in rabbits. The transgenic mice with DQ haplotypes were able to induce B cell response and this was effective in the induction of anti-rLiD1 antibodies important to prevent the venom pathogenesis.…”

B‐cell activation by T‐cell‐independent type 2 antigens as an integral part of the humoral immune response to pathogenic microorganisms

“…This T-cell response could be restored by infusion of a combination of normal mouse Ig and anti-IgM. These findings demonstrate the potential involvement of an idiotypic network (193,194) in which B cells would recruit T cells that showed specificity for MHC-presented idiotypic peptides, and are supported by the important role of Ig idiotypes in the selection of idiotypespecific T cells (195)(196)(197). In addition, Bogen and colleagues have established a functional in vivo idiotypic network consisting of idiotype-expressing B cells and idiotope-specific T cells (198).…”

Characterization of the immunodeficiency of RIIIS/J mice: immune response to polysaccharide antigens

“…PFC were detected by a slide version of the technique of localized hemolysis-in-gel, using indicator sheep erythrocyte (SRBC) coupled with the appropriate immunogen. The coupling of SRBC with SSS-III (9), E. coli 0113 LPS (25), dextran B1355 (33), BL 17, E. coli 055 LPS (15), TNP (44), and the cell wall polysaccharide phosphocholine (PC) extracted from S. pneumoniae R36A or PnC (12) have been described. PnC was provided by M. J. Caulfield (Cleveland Clinic Foundation, Cleveland, Ohio).…”