The Anticancer Activity of Artemisia Judaica Crude Extract in Human Hepatocellular Carcinoma HepG2 Cells by Induction of Apoptosis and Cell Cycle Arrest

Al-Senosy, Neima K.; Ebeed, Naglaa; Salem, Lamiaa M.; Girgis, Shenouda M.; Ahmad, Ekram S.

doi:10.31782/ijcrr.2021.13732

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“…As seen in Figure 2 B, the crude extract significantly increased the cell population at G2/M phase by 2.26-fold change (27.7% compared to control 12.26%). These results agreed with some of previous work which exhibited the apoptosis-inducing activity by arresting cell cycle at G2/M phase [ 19 , 43 , 44 ].…”

Section: Resultssupporting

confidence: 93%

“…Moreover, the cytotoxic activity of the alcoholic crude extract was previously reported against hepatocellular carcinoma (HepG2), breast cancer (MCF-7), and colon tumor (LoVo) cell lines. This was explained through its ability to inhibit cell proliferation, angiogenesis, and inflammation in addition to its a beneficial protective effect against doxorubicin-induced toxicity in mice [ 15 , 16 , 17 , 18 , 19 ]. Both the ethyl acetate and petroleum ether fractions exhibited remarkable renal protective activity in hyperlipidemic and hyperglycemic rats [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro and In Vivo Studies of Anti-Lung Cancer Activity of Artemesia judaica L. Crude Extract Combined with LC-MS/MS Metabolic Profiling, Docking Simulation and HPLC-DAD Quantification

et al. 2021

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Artemisia judaica L. (Family: Asteraceae) exhibited antioxidant, anti-inflammatory, and antiapoptotic effects. The in vitro cytotoxic activity of A. judaica ethanolic extract was screened against a panel of cancer cell lines. The results revealed its cytotoxic activity against a lung cancer (A549) cell line with a promising IC50 of 14.2 μg/mL compared to doxorubicin as a standard. This was confirmed through the downregulation of antiapoptotic genes, the upregulation of proapoptotic genes, and the cell cycle arrest at the G2/M phase. Further in vivo study showed that a solid tumor mass was significantly reduced, with a tumor inhibition ratio of 54% relative to doxorubicin therapy in a Xenograft model. From a chemical point of view, various classes of natural products have been identified by liquid chromatography combined with tandem mass spectrometry (LC-MS/MS). The docking study of the detected metabolites approved their cytotoxic activity through their virtual binding affinity towards the cyclin-dependent kinase 2 (CDK-2) and epidermal growth factor receptor (EGFR) active sites. Finally, A. judaica is a fruitful source of polyphenols that are well-known for their antioxidant and cytotoxic activities. As such, the previously reported polyphenols with anti-lung cancer activity were quantified by high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD). Rutin, quercetin, kaempferol, and apigenin were detected at concentrations of 6 mg/gm, 0.4 mg/gm, 0.36 mg/gm, and 3.9 mg/gm of plant dry extract, respectively. It is worth noting that kaempferol and rutin are reported for the first time. Herein, A. judaica L. may serve as an adjuvant therapy or a promising source of leading structures in drug discovery for lung cancer treatment.

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Section: Resultssupporting

confidence: 93%