The Anticarcinogenic Effect of the Apple Polyphenol Phloretin in an Experimental Rat Model of Hepatocellular Carcinoma

Alansari, Wafa S.; Eskandrani, Areej A.

doi:10.1007/s13369-020-04478-7

Cited by 10 publications

(8 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the fact that chemotherapeutic medications are effective therapies for HCC, their high toxicity, considerable side effects, and long-term drug resistance restrict clinical outcomes, resulting in an increasing death rate 61 . Several lines of evidence have been gathered to support potential HCC therapeutic regimens employing traditional medicinal plant extracts 6 – 14 .…”

Section: Discussionmentioning

confidence: 99%

“…DOX concentrations ranging from 1 to 100 µM have been reported to have potent anticancer activity against a variety of cancer cells, including cervical cancer HeLa cells 69 , murine breast cancer cells 70 , mouse E0771 breast cancer cells 71 , A-172 brain, glioblastoma and mammary gland, adenocarcinoma MCF-7 cells 72 , canine transitional cell carcinoma cells, canine osteosarcoma cells, canine hemangiosarcoma cells 73 , and human liver cancer BEL-7404 cells 74 . However, drug resistance and the occurrence of systemic adverse effects on the heart, liver, kidneys, and testis associated with DOX treatment, as well as its poor application due to its short half-life, limit its utility 14 , 24 – 26 . To overcome these barriers and achieve therapeutic success, some studies recommended a combination regimen of medicinal plant extracts and DOX.…”

Section: Discussionmentioning

confidence: 99%

“…The suppression of adenosine triphosphate (ATP) production, which mediates apoptotic activity, was investigated in HepG2 cells in this study. The extracts, when combined with the lowest dose of doxorubicin (DOX), the anthracycline antibiotic that inhibits topoisomerase II, were hypothesized to improve the anticancer activity of the extract compared to monotherapy, with less adverse toxicity to the heart, liver, kidneys, and testis, as previously reported 14 , 24 – 26 . In addition, HCC development in rats was studied using DEN to evaluate the effect of the extracts from stem bark of C. gigantea and a combination with DOX on the suppression of hepatocarcinogenesis over the course of 4 weeks of treatment.…”

Section: Introductionmentioning

confidence: 87%

“…This report is consistent with the antioxidant activity of extracts from Punica granatum (pomegranate) peel and seed oil 7 , blueberries 8 , lotus Nelumbo nucifera leaves 9 , ajwa dates ( Phoenix dactylifera L.) 10 , and Solanum trilobatum 11 , which inhibited DEN-induced hepatic injury in animal models. Ganoderic acid A isolated from Ganoderma lucidum 12 ; triterpenoid compounds found in the aerial parts of Wedelia calendulacea 13 , and dihydrochalcone flavonoids present in the leaves, bark, and fruit of apple trees decreased oxidative stress and inflammatory responses 14 , which could inhibit carcinogenesis against DEN exposure. Altogether, compounds in the abovementioned plant extracts have been proposed to possess potential anticancer activity.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Calotropis gigantea stem bark extracts inhibit liver cancer induced by diethylnitrosamine

Sawong

Pekthong

Suknoppakit

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Several fractions of Calotropis gigantea extracts have been proposed to have potential anticancer activity in many cancer models. The present study evaluated the anticancer activity of C. gigantea stem bark extracts in liver cancer HepG2 cells and diethylnitrosamine (DEN)-induced primary liver cancer in rats. The carcinogenesis model induced by DEN administration has been widely used to study pathophysiological features and responses in rats that are comparable to those seen in cancer patients. The dichloromethane (CGDCM), ethyl acetate, and water fractions obtained from partitioning crude ethanolic extract were quantitatively analyzed for several groups of secondary metabolites and calactin contents. A combination of C. gigantea stem bark extracts with doxorubicin (DOX) was assessed in this study to demonstrate the enhanced cytotoxic effect to cancer compared to the single administration. The combination of DOX and CGDCM, which had the most potential cytotoxic effect in HepG2 cells when compared to the other three fractions, significantly increased cytotoxicity through the apoptotic effect with increased caspase-3 expression. This combination treatment also reduced ATP levels, implying a correlation between ATP and apoptosis induction. In a rat model of DEN-induced liver cancer, treatment with DOX, C. gigantea at low (CGDCM-L) and high (CGDCM-H) doses, and DOX + CGDCM-H for 4 weeks decreased the progression of liver cancer by lowering the liver weight/body weight ratio and the occurrence of liver hyperplastic nodules, fibrosis, and proliferative cells. The therapeutic applications lowered TNF-α, IL-6, TGF-β, and α-SMA inflammatory cytokines in a similar way, implying that CGDCM had a curative effect against the inflammation-induced liver carcinogenesis produced by DEN exposure. Furthermore, CGDCM and DOX therapy decreased ATP and fatty acid synthesis in rat liver cancer, which was correlated with apoptosis inhibition. CGDCM reduced cleaved caspase-3 expression in liver cancer rats when used alone or in combination with DOX, implying that apoptosis-inducing hepatic carcinogenesis was suppressed. Our results also verified the low toxicity of CGDCM injection on the internal organs of rats. Thus, this research clearly demonstrated a promising, novel anticancer approach that could be applied in future clinical studies of CGDCM and combination therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Calotropis gigantea stem bark extracts inhibit liver cancer induced by diethylnitrosamine

Sawong

Pekthong

Suknoppakit

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…After the establishment of obesity model [8] mice, each group was fed a basal diet. Gastric administration at 9:00 every day: Apple Polyphenol low-dose group (4.2mg/ kGBW/ d), medium-dose group (8.4mg/ kGBW/ d), highdose group (12.6mg/ kGBW /d).…”

Section: Grouping and Administration Of Animalsmentioning

confidence: 99%

Effects of Apple Polyphenol on Fat Metabolism in Mice

Tang

Zhang

et al. 2021

E3S Web Conf.

View full text Add to dashboard Cite

To study the regulation effect of apple polyphenol on fat metabolism in mice. The mice were randomly divided into 5 groups, except the basic control group, the other 4 groups were given high fat diet, body weight was measured once a week, and the obesity model was established. The basal control group and the obesity model group were intragastrically given normal saline (4.2mg/(kgbw.d), The three dose groups were gavaged separately with apple polyphenol 4.2, 8.4, 12.6 mg/(kg bw. d). After 4 consecutive weeks, the body weight of mice, the contents of total cholesterol (TC), total triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA) in serum and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), liver lipase (HL) and lipoprotein lipase (LPL) in serum. should be measured respectively. Apple polyphenol can significantly reduce the body weight, TG, LDL-C, TC and MDA contents, coupled with the atherosclerosis index (AI) of obese mice. It can increase the serum HDL-C content of obese mice, and effectively increase the activities of SOD, GSH-Px, HL and LPL in mice. Apple Polyphenol can reduce blood lipid and prevent atherosclerosis via regulating fat metabolism in mice.

show abstract

Role of phytoconstituents in the hepatocellular carcinoma management: current perspective, challenges, and future perspectives

Sharbidre

2022

Theranostics and Precision Medicine for the Management of Hepatocellular Carcinoma, Volume 3

View full text Add to dashboard Cite

The Anticarcinogenic Effect of the Apple Polyphenol Phloretin in an Experimental Rat Model of Hepatocellular Carcinoma

Cited by 10 publications

References 68 publications

Calotropis gigantea stem bark extracts inhibit liver cancer induced by diethylnitrosamine

Calotropis gigantea stem bark extracts inhibit liver cancer induced by diethylnitrosamine

Effects of Apple Polyphenol on Fat Metabolism in Mice

Role of phytoconstituents in the hepatocellular carcinoma management: current perspective, challenges, and future perspectives

Contact Info

Product

Resources

About