The anticoagulant ability of ferulic acid and its applications for improving the blood compatibility of silk fibroin

Wang, Song; Gao, Zhen; Xiaomeng, Chen; Lian, Xiaojie; Zhu, Hesun; Zheng, Jun; Sun, Li‐Zhong

doi:10.1088/1748-6041/3/4/044106

Cited by 33 publications

(20 citation statements)

References 17 publications

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“…To date, a number of studies have evaluated the hemocompatibility of silk [19-21], silk alloys composed of silk and collagen [22, 23], silk and keratin [24], silk and chitosan [25], silk blends with either heparin [22] or ferulic acid [26], or silk modified chemically through sulfation [27, 28] or grafting with S-carboxymethyl keratin [24] or heparin [25]. We previously determined the impact of processing parameters on the hemocompatibility of pure silk films by using human whole blood and reference materials [29].…”

Section: Introductionmentioning

confidence: 99%

Multifunctional silk–heparin biomaterials for vascular tissue engineering applications

et al. 2014

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Over the past 30 years, silk has been proposed for numerous biomedical applications that go beyond its traditional use as a suture material. Silk sutures are well tolerated in humans, but the use of silk for vascular engineering applications still requires extensive biocompatibility testing. Some studies have indicated a need to modify silk to yield a hemocompatible surface. This study examined the potential of low molecular weight heparin as a material for refining silk properties by acting as a carrier for vascular endothelial growth factor (VEGF) and improving silk hemocompatibility. Heparinized silk showed a controlled VEGF release over 6 days; the released VEGF was bioactive and supported the growth of human endothelial cells. Silk samples were then assessed using a humanized hemocompatibility system that employs whole blood and endothelial cells. The overall thrombogenic response for silk was very low and similar to the clinical reference material polytetrafluoroethylene. Despite an initial inflammatory response to silk, apparent as complement and leukocyte activation, the endothelium was maintained in a resting, anticoagulant state. The low thrombogenic response and the ability to control VEGF release support the further development of silk for vascular applications.

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Section: Introductionmentioning

confidence: 99%

Multifunctional silk–heparin biomaterials for vascular tissue engineering applications

et al. 2014

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“…A number of studies reported rapid blood coagulation when exposed to soluble silk fibroin and silk-collagen scaffolds [14,15] and reduced clotting for functionalised silk substrates for example silk blends with either heparin, ferulic acid or chemical modification of silk such as sulphation or grafting of S-carboxymethyl keratin [14–17]. Qualitative methods suggested higher serum protein adsorption and fibrin polymerisation for silk fibres when compared to methanol treated silk films [18,19].…”

Section: Introductionmentioning

confidence: 99%

Impact of processing parameters on the haemocompatibility of Bombyx mori silk films

Seib

Maitz

et al. 2012

Biomaterials

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Silk has traditionally been used for surgical sutures due to its lasting strength and durability; however, the use of purified silk proteins as a scaffold material for vascular tissue engineering goes beyond traditional use and requires application-orientated biocompatibility testing. For this study, a library of Bombyx mori silk films was generated and exposed to various solvents and treatment conditions to reflect current silk processing techniques. The films, along with clinically relevant reference materials, were exposed to human whole blood to determine silk blood compatibility. All substrates showed an initial inflammatory response comparable to polylactide-co-glycolide (PLGA), and a low to moderate haemostasis response similar to polytetrafluoroethylene (PTFE) substrates. In particular, samples that were water annealed at 25 °C for 6 h demonstrated the best blood compatibility based on haemostasis parameters (e.g. platelet decay, thrombin-antithrombin complex, platelet factor 4, granulocytes-platelet conjugates) and inflammatory parameters (e.g. C3b, C5a, CD11b, surface-associated leukocytes). Multiple factors such as treatment temperature and solvent influenced the biological response, though no single physical parameter such as β-sheet content, isoelectric point or contact angle accurately predicted blood compatibility. These findings, when combined with prior in vivo data on silk, support a viable future for silk-based vascular grafts.

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“…[5][6][7] Ferulic acid have also been used to improve haemostatics during silk weaving. 8,9 Phenolic acids which includes about half a dozen chemicals in Ferula spp. possess emotional improving and antidepressant activities.…”

Section: Discussionmentioning

confidence: 99%

Psychopharmacological characterization of effects of Ferula asafoetida linn. formulation in mouse on a Y-maze, EPM, and open field apparatus

Narayanan

Gilbert

Ravichandran

et al. 2017

Int J Basic Clin Pharmacol

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Background: Asafoetida is an herbal food supplement that has numerous peripheral pharmacological actions. Its central nervous system effects have not yet been completely elucidated in both animals and human participants. The study focuses on deciphering the effects of this Herbaceutical with that of Donepezil and vitamin C in mouse models of short term novelty object recognition memory and open field test.Methods: The study was conducted in two stages - a pilot stage which assessed the most effective dose of asafoetida on cognition, followed by a post-pilot study phase wherein its effects at that dose were compared with those of Donepezil and vitamin C. Novelty Preference Test (NPT) on y-maze and Open field test on Open field box were used to evaluate the effects of the formulation on memory and motor activity in mice.Results: A dose of 400mg/day of asafoetida orally was found to improve memory significantly in comparison to 200mg/day in mice. Asafoetida showed a greater potency in improving memory than Donepezil and vitamin C. After 11 days of daily treatment with Asafoetida commercial powder, more than fifty percent of the mice showed an increase in recognition index of 0.7-0.8 as compared to 0.55 at baseline.Conclusions: Asafoetida powder has nootropic efficacy in mouse model - this has to be explored mechanistically and pharmaceutically to enable the psychopharmacological characterization of the active principles and its pharmacological effects in the central nervous system.

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The anticoagulant ability of ferulic acid and its applications for improving the blood compatibility of silk fibroin

Cited by 33 publications

References 17 publications

Multifunctional silk–heparin biomaterials for vascular tissue engineering applications

Multifunctional silk–heparin biomaterials for vascular tissue engineering applications

Impact of processing parameters on the haemocompatibility of Bombyx mori silk films

Psychopharmacological characterization of effects of Ferula asafoetida linn. formulation in mouse on a Y-maze, EPM, and open field apparatus

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