The Antidiabetic Effect of Mesenchymal Stem Cells vs. Nigella sativa Oil on Streptozotocin Induced Type 1 Diabetic Rats

Abstract

Introduction: Diabetes is a chronic metabolic disorder that has no definitive cure till present. Currently; regenerative medicine using Mesenchymal Stem Cells (MSCs) offers promising treatment. Meanwhile, Nigella Sativa Oil (NSO) shows effectiveness for medication of various illnesses.Objective: This study compares the antidiabetic effect of MSCs vs. NSO on streptozotocin induced type 1 diabetes (T1D) in rat model. Methods:Human cord blood samples were collected. Isolated mononuclear cells were cultured and incubated to isolate MSCs. For experimental animals; after induction of T1D using multiple low dose streptozotocin (MLD-STZ), diabetic rats were divided into 6 groups (n =10); diabetic group 1 and 2 (sacrificed 15 and 30 days post-induction, consequently), NSO treated groups 1 and 2 (received daily NSO I.P. injection, sacrificed 15 and 30 days postinduction, consequently), MSCs treated groups 1 and 2 (72 hours post-induction, animals were treated with twice intravenous injection of 1×106 cells/rat, 24 hours apart, without immunosuppression, sacrificed 15 and 30 days of induction, consequently).Results: Low blood glucose levels were detected in both MSCs and NSO groups, which reached near normal levels within 15 days. This was accompanied by improvement of the histopathological changes and increase in the immunreactive insulin secreting cells, obviously detected after 30 days.

Adverse effects of energy drink on rat pancreas and the therapeutic role of each of bone marrow mesenchymal stem cells and Nigella Sativa oil

“…In the present work, fluorescent labelled-BMSCs homing was identified in the pancreatic lobules and in the interlobular spaces. This is similar to previous observation [31].…”

“…In current BMSCs-injected rats, the serum glucose is significantly decreased whereas the serum insulin is significantly increased. These findings confirm previous studies [29][30][31].…”

“…The NS-injected rats have demonstrated obvious islet and acinar improvement. These findings are consistent with previous findings [21,31]. The protective effect of NS could be attributed to its role in reduction of inflammatory cytokines and lipid peroxidation [12,48].…”

Effect of Stem Cells and Gene Transfected Stem Cells Therapy on the Pancreas of Experimentally Induced Type 1 Diabetes

“…Diabetes is one of the most recognizable endocrine metabolic disorders characterized by chronic hyperglycemia. Over time, it increased the risk of heart disease, stroke, diabetic nephropathy and blindness (2). Type 1 diabetes (T1DM), the chronic autoimmune disease, results from the destruction of the in-sulin producing beta (β) cells of the pancreas, leading to a gradual decrease in β-cell mass.…”