The Antifungal Activity of Loquat (Eriobotrya japonica Lindl.) Leaves Extract Against Penicillium digitatum

Shen, Yuting; Chen, Chuying; Cai, Nan; Yang, Ruopeng; Chen, Jinyin; Kahramanoğlu, İbrahim; Okatan, Volkan; Rengasamy, Kannan R.R.; Wan, Chunpeng

doi:10.3389/fnut.2021.663584

Cited by 17 publications

(5 citation statements)

References 43 publications

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“…This is consistent with the antifungal mechanism of the Eriobotrya japonica leaf extract and the Chinese herbal extract compound (CHEC) on MDA. 67,68 The destruction of the cell membrane was also confirmed through the phenomenon of the mycelium being transparent and ruined by light microscopy. In the meantime, according to the increased transparency and breaking of the mycelium, we speculate that the petroleum ether extract may also do harm to the cell wall.…”

Section: ■ Discussionmentioning

confidence: 83%

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Zhao

Wang

et al. 2022

ACS Omega

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Rice sheath blight (RSB) caused by Rhizoctonia solani is one of the most destructive diseases of rice (Oryza sativa). Although chemical fungicides are the most important control methods, their long-term unreasonable application has brought about problems such as environmental pollution, food risks, and non-target poisoning. Therefore, considering the extraction of fungistatic substances from plants may be an alternative in the future. In this study, we found that the Moutan cortex ethanol extract has excellent antifungal activity against R. solani, with a 100% inhibition rate at 1000 μg/mL, which aroused our great exploration interest. In-depth exploration found that the antifungal active ingredients of M. cortex were mainly concentrated in the petroleum ether extract of the M. cortex ethanol extract, which still maintained a 100% inhibition rate with 250 μg/mL, and its effective medium concentration (EC 50 ) was 145.33 μg/mL against R. solani. Through the measurement of extracellular relative conductivity and OD 260 , the petroleum ether extract induced leakage of intracellular electrolytes and nucleic acids, indicating that the cell membrane was ruined. Therefore, we preliminarily determined that the cell membrane may be the target of the petroleum ether extract. Moreover, we found that petroleum ether extract reduced the content of ergosterol, a component of the cell membrane, which may be one of the reasons for the cell membrane destruction. Furthermore, the increase of MDA content would lead to membrane lipid peroxidation, further aggravating membrane damage, resulting in increased membrane permeability. Also, the destruction of the cell membrane was observed by the phenomenon of the mycelium being transparent and broken. In conclusion, this is the first report of the M. cortex petroleum ether extract exhibiting excellent antifungal activity against R. solani. The effect of the M. cortex petroleum ether extract on R. solani may be on the cell membrane, inducing the disorder of intracellular substances and metabolism, which may be one of the antifungal mechanisms against R. solani.

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Section: ■ Discussionmentioning

confidence: 83%

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Zhao

Wang

et al. 2022

ACS Omega

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“…All strains showed similar biofilm development, maturation, and diffusion stages, but the maximum biofilm biomass formed by the four mutants was remarkably smaller than the WT strain (p < 0.001). Cell membranes serve as selective semi-permeable barriers whose integrity, fluid and selective permeation control the movement of various substances, playing a piv role in microbial growth and pathogenicity [31]. Based on the above results, we fur asked whether the deletion of the VpaChn25_RS25055, VpaChn25_0713, VpaChn25_0714 genes would influence bacterial cell membrane structure (Figures 5 S13).…”

Section: 4mentioning

confidence: 99%

“…2.6. Cell Surface Hydrophobicity, Cell Membrane Permeability, and Fluidity of the ∆VpaChn25_RS25055, ∆VpaChn25_0713, ∆VpaChn25_0714, and ∆VpaChn25_RS25055-0713-0714 Mutants Cell membranes serve as selective semi-permeable barriers whose integrity, fluidity, and selective permeation control the movement of various substances, playing a pivotal role in microbial growth and pathogenicity [31]. Based on the above results, we further asked whether the deletion of the VpaChn25_RS25055, VpaChn25_0713, and VpaChn25_0714 genes would influence bacterial cell membrane structure (Figures 5 and S13).…”

Section: 4mentioning

confidence: 99%

Biological Function of Prophage-Related Gene Cluster ΔVpaChn25_RS25055~ΔVpaChn25_0714 of Vibrio parahaemolyticus CHN25

Zhao,

Xu,

Yang

et al. 2024

IJMS

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Vibrio parahaemolyticus is the primary foodborne pathogen known to cause gastrointestinal infections in humans. Nevertheless, the molecular mechanisms of V. parahaemolyticus pathogenicity are not fully understood. Prophages carry virulence and antibiotic resistance genes commonly found in Vibrio populations, and they facilitate the spread of virulence and the emergence of pathogenic Vibrio strains. In this study, we characterized three such genes, VpaChn25_0713, VpaChn25_0714, and VpaChn25_RS25055, within the largest prophage gene cluster in V. parahaemolyticus CHN25. The deletion mutants ΔVpaChn25_RS25055, ΔVpaChn25_0713, ΔVpaChn25_0714, and ΔVpaChn25_RS25055-0713-0714 were derived with homologous recombination, and the complementary mutants ΔVpaChn25_0713-com, ΔVpaChn25_0714-com, ΔVpaChn25_RS25055-com, ΔVpaChn25_RS25055-0713-0714-com were also constructed. In the absence of the VpaChn25_RS25055, VpaChn25_0713, VpaChn25_0714, and VpaChn25_RS25055-0713-0714 genes, the mutants showed significant reductions in low-temperature survivability and biofilm formation (p < 0.001). The ΔVpaChn25_0713, ΔVpaChn25_RS25055, and ΔVpaChn25_RS25055-0713-0714 mutants were also significantly defective in swimming motility (p < 0.001). In the Caco-2 model, the above four mutants attenuated the cytotoxic effects of V. parahaemolyticus CHN25 on human intestinal epithelial cells (p < 0.01), especially the ΔVpaChn25_RS25055 and ΔVpaChn25_RS25055-0713-0714 mutants. Transcriptomic analysis showed that 15, 14, 8, and 11 metabolic pathways were changed in the ΔVpaChn25_RS25055, ΔVpaChn25_0713, ΔVpaChn25_0714, and ΔVpaChn25_RS25055-0713-0714 mutants, respectively. We labeled the VpaChn25_RS25055 gene with superfolder green fluorescent protein (sfGFP) and found it localized at both poles of the bacteria cell. In addition, we analyzed the evolutionary origins of the above genes. In summary, the prophage genes VpaChn25_0713, VpaChn25_0714, and VpaChn25_RS25055 enhance V. parahaemolyticus CHN25’s survival in the environment and host. Our work improves the comprehension of the synergy between prophage-associated genes and the evolutionary process of V. parahaemolyticus.

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“…Loquat fruit is rich in essential dietary nutrients including sugars, protein, carotenoids, ber, vitamins A and C, and minerals (Tian et al 2011;Li et al 2016). Loquat leaves are rich in ber, minerals, and vitamins B2, B6, and B12, and have several medicinal properties such as antioxidant, antidiabetic, antitumoral, anti-in ammatory, antinociceptive, and antimicrobial (Cha et al 2011; Khouya et al 2022; Kuraoka-Oliveira et al 2020; Shen et al 2021). These properties lead loquat to be commercially cultivated in more than 20 countries including China, India, Australia, Brazil, Italy, Spain, Turkey, and Japan (Tian et al 2007(Tian et al , 2011.…”

Section: Introductionmentioning

confidence: 99%

Colletotrichum species associated with loquat anthracnose in Kagawa and Tokushima prefectures, Japan

AKIMITSU,

Poti,

Kisaki

et al. 2023

Preprint

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Anthracnose caused by Colletotrichum species is one of the serious diseases of loquat, but diversification of the species of Colletotrichum associated with loquat in Japan has not been adequately surveyed. In this study, 46 isolates were isolated from diseased leaves and fruits of loquat in Kagawa and Tokushima prefectures in 2017 and 2018. Using a combination of morphological features and molecular approaches, seven Colletotrichum species were identified: C. fioriniae, C. nymphaeae, C. karsti, C. aenigma, C. fructicola, C. gloeosporioides s. s., and C. siamense. The most prevalent species was C. karsti followed by C. fioriniae and C. gloeosporioides s. s., accounting for 43.5, 21.7, and 13.0%, respectively. All identified species were confirmed to be the causal agents of loquat anthracnose by applying Koch’s postulates. Moreover, potential cross-infection from loquat to kiwifruit, satsuma mandarin, persimmon, and peach was found, in which loquat isolates of C. fioriniae, C. fructicola, and C. siamense caused symptoms on all the tested plants. This report is the first on C. karsti and C. aenigma associated with loquat anthracnose and the first record of C. fructicola and C. gloeosporioides s. s. in Japan, providing crucial information for epidemiology.

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The Antifungal Activity of Loquat (Eriobotrya japonica Lindl.) Leaves Extract Against Penicillium digitatum

Cited by 17 publications

References 43 publications

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Biological Function of Prophage-Related Gene Cluster ΔVpaChn25_RS25055~ΔVpaChn25_0714 of Vibrio parahaemolyticus CHN25

Colletotrichum species associated with loquat anthracnose in Kagawa and Tokushima prefectures, Japan

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