The antifungal properties of a 2S albumin-homologous protein from passion fruit seeds involve plasma membrane permeabilization and ultrastructural alterations in yeast cells

Agizzio, Ana Paula; Cunha, Maura Da; Carvalho, André O.; Oliveira, Marco Antônio de; Ribeiro, Suzanna F. F.; Gomes, Valdirene Moreira

doi:10.1016/j.plantsci.2006.06.001

Cited by 36 publications

(32 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4b, f). Different plant proteins/peptides have also been found to permeabilize membranes and to modulate ion flux across membranes and have since become popular models for understanding how ion channel proteins function (Abad et al 1996;Regente et al 2005;Agizzio et al 2006).…”

Section: Resultsmentioning

confidence: 99%

“…During recent years, many plant proteins and peptides that can inhibit fungal development have been isolated and characterized. Examples of these compounds include 2S albumins (Agizzio et al 2003(Agizzio et al , 2006Ribeiro et al 2011), thionins (García-Olmedo et al 1998Stec 2006;Berrocal-Lobo et al 2009), defensins (Thevissen et al 2003;Games et al 2008;Mello et al 2011;Rogozhin et al 2011), lipid transfer proteins (LTPs) (Diz et al 2006;Carvalho and Gomes 2007;Zottich et al 2011) and proteinase inhibitors (PIs) (Terras et al 1993;Macedo et al 2007;Ribeiro et al 2007;Lopes et al 2009). …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Ribeiro

Silva

Cunha

et al. 2011

Antonie van Leeuwenhoek

Self Cite

View full text Add to dashboard Cite

A 6,000 Da peptide, named CaTI, was isolated from Capsicum annuum L. seeds and showed potent inhibitory activity against trypsin and chymotrypsin. The aim of this study was to determine the effect of CaTI on Saccharomyces cerevisiae, Candida albicans, Candida tropicalis and Kluyveromyces marxiannus cells. We observed that CaTI inhibited the growth of S. cerevisiae, K. marxiannus as well as C. albicans and induced cellular agglomeration and the release of cytoplasmic content. No effect on growth was observed in C. tropicalis but morphological changes were noted. In the spot assay, different degrees of sensitivity were shown among the strains and concentrations tested. Scanning electron microscopy showed that S. cerevisiae, K. marxiannus and C. albicans, in the presence of CaTI, exhibited morphological alterations, such as the formation of pseudohyphae, cellular aggregates and elongated forms. We also show that CaTI induces the generation of nitric oxide and interferes in a dose-dependent manner with glucose-stimulated acidification of the medium mediated by H(+)-ATPase of S. cerevisiae cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Ribeiro

Silva

Cunha

et al. 2011

Antonie van Leeuwenhoek

Self Cite

View full text Add to dashboard Cite

show abstract

“…SEM was performed as described already in the literature [10]. Acinetobacter baumannii was grown in MH agar at 37 ºC and in a saline solution containing 0.2 % Tween-80.…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Antibacterial Activity and Mode of Action of <i>Mentha arvensis</i> Ethanol Extract against Multidrug-Resistant <i>Acinetobacter baumannii</i>

Zhang¹,

Xu²,

Wei³

et al. 2015

Trop. J. Pharm Res

View full text Add to dashboard Cite

show abstract

“…The fixed material was mounted on aluminum stubs using double-sided carbon tape and coated with gold in a sputter coater system (EMITECH K575X, UK) for 420 s at 10 mA. Finally, the samples were examined with the scanning electron microscope at an accelerating voltage of 10 kV as described (Agizzio et al, 2006).…”

Section: Release Of Cellular Materialsmentioning

confidence: 99%

Antifungal activity of eugenol against Botrytis cinerea

Wang¹,

Zhang

Chen³

et al. 2010

Trop. plant pathol.

111

View full text Add to dashboard Cite

The antifungal properties of eugenol were tested against ten plant pathogenic fungal species and detailed studies were conducted regarding in vitro activity of eugenol on Botrytis cinerea. The EC 50 value of eugenol on mycelial radial growth of B. cinerea was 38.6 µg/ mL; however, eugenol had no bioactivity against conidia germination. B. cinerea hyphae treated with eugenol showed strong propidium iodide fluorescence in the cytosol. Eugenol increased the concentration of potassium ion and cellular materials in the medium. Furthermore, light and scanning electron microscopy observations on hyphae exposed to eugenol revealed considerable morphological alterations in hyphae, such as cytoplasmic coagulation, vacuolation, and hyphal shriveling. Eugenol induced the generation of H 2 O 2 and increased free Ca 2+ in the cytoplasm. These results strongly support the idea that the antifungal activity of eugenol is due to membrane binding and permeability alteration, leading to destabilization and disruption of the plasma membrane.

show abstract

The antifungal properties of a 2S albumin-homologous protein from passion fruit seeds involve plasma membrane permeabilization and ultrastructural alterations in yeast cells

Cited by 36 publications

References 27 publications

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Antibacterial Activity and Mode of Action of <i>Mentha arvensis</i> Ethanol Extract against Multidrug-Resistant <i>Acinetobacter baumannii</i>

Antifungal activity of eugenol against Botrytis cinerea

Contact Info

Product

Resources

About