The antigenic character of zinc-binding proteins and their localization in boar reproductive tract--a preliminary study.

Abstract

In this study immunoelectrophoretic and double immunodiffusion analyses were used to investigate the antigenic character of zinc-binding proteins (ZnBPs), whereas the indirect immunofluorescence technique was used to identify their origin in boar reproductive tract. The mmunoelectrophoretic analysis of ZnBPs of the seminal plasma resulted in the appearance of three antigenic protein complexes, while specific immunoreactivity patterns of the anti-ZnBP serum were detected by double immunodiffusion analysis. Indirect immunofluorescence technique confirmed that ZnBPs were secreted by different reproductive tract tissues, suggesting their contributions to the seminal plasma.

Proteomic Characterization of Zinc‐Binding Proteins of Canine Seminal Plasma

“…; Mogielnicka‐Brzozowska et al. , ). In our laboratory, we isolated and initially characterized zinc‐binding proteins of canine seminal plasma (Mogielnicka‐Brzozowska et al.…”

“…Zinc affects spermatozoa motility and acrosome reaction (Riffo et al 1992;Henkel et al 1999). Seminal plasma zinc-binding proteins have been relatively extensively characterized in humans and boar (Siciliano et al 2000;Mogielnicka-Brzozowska et al 2011;Mogielnicka-Brzozowska et al 2012;Mogielnicka-Brzozowska et al 2013, 2014b. In our laboratory, we isolated and initially characterized zinc-binding proteins of canine seminal plasma (Mogielnicka-Brzozowska et al 2012).…”