The antimicrobial activity of fruits from some cultivar varieties of Rubus idaeus and Rubus occidentalis

Abstract

Raspberries, derived from different cultivar varieties, are a popular ingredient of everyday diet, and their biological activity is a point of interest for researchers. The ethanol-water extracts from four varieties of red (Rubus idaeus'Ljulin', 'Veten', 'Poranna Rosa') and black (Rubus occidentalis'Litacz') raspberries were evaluated in the range of their antimicrobial properties as well as phenolic content - sanguiin H-6, free ellagic acid and anthocyanins. The antimicrobial assay was performed with the use of fifteen strains of bacteria, both Gram-negative and Gram-positive. The antimicrobial activity of the extracts varied and depended on the analysed strain of bacteria and cultivar variety, with the exception of Helicobacter pylori, towards which the extracts displayed the same growth inhibiting activity. Two human pathogens Corynebacterium diphtheriae and Moraxella catarrhalis proved to be the most sensitive to raspberry extracts. Contrary to the extracts, sanguiin H-6 and ellagic acid were only active against eight and nine bacterial strains, respectively. The determined MIC and MBC values of both compounds were several times lower than the tested extracts. The highest sensitivity of Corynebacterium diphtheriae to extracts from both black and red raspberries may be due to its sensitivity to sanguiin H-6 and ellagic acid.

In vitro Effects of Anthocyanidins on Sinonasal Epithelial Nitric Oxide Production and Bacterial Physiology

“…26 Because NO is an important component of sinonasal innate immunity, 19,27,28 we hypothesized that anthocyanidins may activate sinonasal T2Rs to generate NO. In addition, anthocyanidin-rich plant extracts have antibacterial properties against Staphylococcus aureus, 29,30 Escherichia coli, 29,31 Corynebacterium diphtheria, 32 Moraxella catarrhalis, 32 Bacillus cereus, 33 and Pseudomonas aeruginosa. 34 However, other studies have indicated that anthocyanidins do not have antibacterial effects against B. cereus, 34 E. coli, 34,35 or P. aeruginosa, 30 or that anthocyanidins only reduce inflammation.…”

Dietary amelioration of Helicobacter infection

“…Leaves of the tree Moringa oleifera , widely consumed in some tropical regions, also have anti-ulcer activity (reviewed in [149]), and certain glucosinolates/isothiocyanates from Moringa have strong antibacterial activity in vitro against H. pylori and a variety of other human pathogens [118,149]. Okra also has anti- H. pylori activity in vitro , thought to be an anti-adhesive effect of okra polysaccharides [150], and it is reported to provide some protection against duodenal ulcer [154]. Even a seaweed, the brown alga Cladosiphon fucoidan , has anti- H. pylori activity in vitro and in gerbils [136], also speculated to be due to the anti-adhesive effect of the alga’s glucuronic acid-rich polysaccharide (fucoidan).…”

“…The second study, by Gottland and colleagues in Chile, showed that administration of cranberry juice for three weeks inhibited H. pylori about 15% of asymptomatic, colonized children, and that in most subjects who became negative (as measured by UBT), the clearing effect did not persist following cessation of consumption [80]. Furthermore, grape skin/seed extracts and wine preparations [128], (recently reviewed by Friedman [127]), pomegranate fruit and ellagic acid-rich juice (recently reviewed by Colombo [52]), apple [126], and Rubus idaeus and R. occicentalis fruit [154], have all been shown to have antimicrobial activity against H. pylori in vitro . Whereas animal models have been used to demonstrate efficacy of some of these fruit products, no trials currently listed on the USA government website ClinicalTrials.gov, propose to evaluate effects on H. pylori colonization.…”

Potentilla and Rubus medicinal plants

“…Ellagic acid derivatives from R. ulmifolius inhibit S. aureus biofilm formation and improve response to antibiotics (Quave et al, 2012). Two human pathogens Corynebacterium diphtheriae and Moraxella catarrhalis were most sensitive to raspberry extracts (Krauze- Baranowska et al, 2014). All the isolated polyphenols as well as the R. ulmifolius leaf extract showed antibacterial activity against two H. pylori strains (Martini et al, 2009).…”