The antimicrobial activity of the alcohols fromMusca domestica

Abstract

SUMMARYInformation on the stimulatory and inhibitory effects of cuticular alcohols on growth and virulence of insecticidal fungi is unavailable. Therefore, we set out to describe the content of cuticular and internal alcohols in the body of housefly larvae, pupae, males and females. The total cuticular alcohols in larvae, males and females of Musca domestica were detected in comparable amounts (4.59, 3.95 and 4.03gg -1 insect body, respectively), but occurred in smaller quantities in pupae (2.16gg -1). The major free alcohol in M. domestica larvae was C 12:0 (70.4%). Internal alcohols of M. domestica larvae were not found. Among cuticular pupae alcohols, C 12:0 (31.0%) was the most abundant. In the internal lipids of pupae, only five alcohols were identified in trace amounts. The most abundant alcohol in males was C 24:0 (57.5%). The percentage content of cuticular C 24:0 in males and females (57.5 and 36.5%, respectively) was significantly higher than that of cuticular lipids in larvae and pupae (0.9 and 5.6%, respectively). , respectively). For isolated alcohols, antimicrobial activity against 10 reference strains of bacteria and fungi was determined. Individual alcohols showed approximately equal activity against fungal strains. C 14:0 was effective against gram-positive bacteria, whereas gram-negative bacteria were resistant to all tested alcohols. Mixtures of alcohols found in cuticular lipids of larvae, pupae, males and females of M. domestica generally presented higher antimicrobial activity than individual alcohols. In contrast, crude extracts containing both cuticular and internal lipids showed no antifungal activity against the entomopathogenic fungus Conidiobolus coronatus, which efficiently kills adult house flies. Only two alcohols were present in the internal lipids of males in trace amounts

Effect of Conidiobolus coronatus on the Cuticular and Internal Lipid Composition of Tettigonia viridissima Males

“…Alcohols have been identified in the cuticular lipids of A. pisum Bemisia tabaci (adults), Trialeurodes vaporariorum (adults) , Locusta migratoria migratoriodes (adults), Schistocerca gregaria (adults) , L. sericata males and females , C. vomitoria , and Musca domestica .…”

Effect of exposure to chlorpyrifos on the cuticular and internal lipid composition of Blattella germanica males

“…However, the following acids occurred in high concentrations in both groups of males after exposure to the presence of the insecticide: C16:0 and C18:1, C16. Literature data indicate the presence of higher concentrations in the internal lipids than in the case of surface lipids (Gołębiowski, ; Gołębiowski, ; Gołębiowski, ). In the majority of the insects they accounted for over 90% of the total fatty acids.…”

Comparison of the organic compounds composition of Hylobius abietis males and females before and after exposure to Beauveria bassiana infection

“…Short‐chain free FA extracted from Heliotis zea larvae inhibit the growth of B. bassiana (Smith & Grula, ). Moreover, alcohols extracted from cuticule and fat body of M. domestica inhibit bacterial and fungal growth (Gołębiowski et al ., ).…”