The antimicrobial peptide MPX kills Actinobacillus pleuropneumoniae and reduces its pathogenicity in mice

Wang, Lei; Zhao, Xueqin; Zhu, Chun-Ling; Zhao, Yaya; Liu, Shuangshuang; Xia, Xiaojing; Liu, Xin; Zhang, Huihui; Xu, Yong; Hang, Bolin; Sun, Yi-Ming; Chen, Shijun; Jiang, Jinqing; Bai, Yueyu; Zhang, Gaiping; Lei, Liancheng; Richard, Langford Paul; Fotina, Hanna; Hu, Jianhe

doi:10.1016/j.vetmic.2020.108634

Cited by 19 publications

(17 citation statements)

References 36 publications

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“…Antimicrobial peptides, as an important part of the natural immune system, possess good anti-inflammatory activity ( Wang et al, 2020 ). Long-term and excessive production of proinflammatory cytokines may lead to intestinal damage and high energy requirements ( Lee et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“… Henriksen et al (2014) found that modulating peptide hydrophobicity by introducing an unnatural amino acid with an octyl side chain via amino acid substitution at positions 1, 8, and 14 increased the bactericidal potency of the antimicrobial peptide MPX ( Henriksen et al, 2014 ). We previously showed that MPX had good antimicrobial activity against Actinobacillus pleuropneumoniae infections, reduced the colonization of A. pleuropneumoniae in the lungs, alleviated the symptoms of pneumonia, and improved the survival rate of mice ( Wang et al, 2020 ). However, the effects of MPX on E. coli infections and on the regulation of E. coli -induced inflammation and intestinal disruption remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier

et al. 2021

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Escherichia coli can cause intestinal diseases in humans and livestock, destroy the intestinal barrier, exacerbate systemic inflammation, and seriously threaten human health and animal husbandry development. The aim of this study was to investigate whether the antimicrobial peptide mastoparan X (MPX) was effective against E. coli infection. BALB/c mice infected with E. coli by intraperitoneal injection, which represents a sepsis model. In this study, MPX exhibited no toxicity in IPEC-J2 cells and notably suppressed the levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), myeloperoxidase (MPO), and lactate dehydrogenase (LDH) released by E. coli. In addition, MPX improved the expression of ZO-1, occludin, and claudin and enhanced the wound healing of IPEC-J2 cells. The therapeutic effect of MPX was evaluated in a murine model, revealing that it protected mice from lethal E. coli infection. Furthermore, MPX increased the length of villi and reduced the infiltration of inflammatory cells into the jejunum. SEM and TEM analyses showed that MPX effectively ameliorated the jejunum damage caused by E. coli and increased the number and length of microvilli. In addition, MPX decreased the expression of IL-2, IL-6, TNF-α, p-p38, and p-p65 in the jejunum and colon. Moreover, MPX increased the expression of ZO-1, occludin, and MUC2 in the jejunum and colon, improved the function of the intestinal barrier, and promoted the absorption of nutrients. This study suggests that MPX is an effective therapeutic agent for E. coli infection and other intestinal diseases, laying the foundation for the development of new drugs for bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier

et al. 2021

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“…30%, 50%, 70%, 80%, 90%, 95%, 100% alcohol gradient dehydration respectively, each time interval of 15 min. After the slides are dry, observe the formation of biofilm under scanning electron microscope (Wang L. et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

The Mechanism of Antimicrobial Peptide MPX against Enterohemorrhagic Escherichia coli in Vitro

Дзяо¹,

Фотіна²,

Ванг³

et al. 2021

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Escherichia coli is a facultative anaerobic bacteria that exists in the gastrointestinal tract of humans and animals. It can cause diarrhea, enteritis, destruction of the host's intestinal barrier, and intestinal microecological disturbances. In recent years, due to the abuse of traditional antibiotics, a variety of drug-resistant strains and super bacteria have emerged in an endless stream. Therefore, there is an urgent need to find new alternatives to antibiotics. Antimicrobial peptides are a type of small peptides produced when organisms resist the invasion of foreign microorganisms. They are considered to be the best alternative to antibiotics which has become a research hotspot in recent years. The antimicrobial peptide MPX is extracted from wasp venom and has a good bactericidal effect on many bacteria. To explore the effect of MPX against E. coli. The function of MPX against E. coli was detected by MIC, plate count, propidium iodide, NPN and DiSC3(5) permeability testing, immunofluorescence microscope observation, and the impact of MPX stability by temperature, pH, ion. In this study, the results found that MPX has good antibacterial activity against E. coli, and the minimum inhibitory concentration (MIC) was 31.25 ug/mL. MPX bactericidal kinetics study found that MPX had good bactericidal activity within 6 hours. Bacterial permeability studies have shown that MPX could increase the permeability of bacteria, leading to an increase in the protein content of the bacterial supernatant. In addition, NPN, PI and DiSC3(5) results showed that the fluorescence value was positively correlated with MPX. The stability test of MPX found that salt ions, temperature, pH, etc. have a slight influence on its effect. In addition, scanning electron microscopy results showed that the bacteria became smaller and the contents leaked after the action of MPX. The above results showed that MPX has a good bactericidal activity in vitro, laying the foundation for the development of new drugs for the treatment of bacterial infections.

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“…Venoms and their peptides from different animals or organisms such as bees, snakes, wasps, and scorpions, represent promising antimicrobial agents against various microbial pathogenesis [7][8][9][10][11][12]. BV is the venomous cocktail secreted by honeybee workers' poison glands as a protection mechanism [13].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Properties of Apis mellifera’s Bee Venom

El-Seedi

El-Wahed

Yosri

et al. 2020

Toxins

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Bee venom (BV) is a rich source of secondary metabolites from honeybees (Apis mellifera L.). It contains a variety of bioactive ingredients including peptides, proteins, enzymes, and volatile metabolites. The compounds contribute to the venom’s observed biological functions as per its anti-inflammatory and anticancer effects. The antimicrobial action of BV has been shown in vitro and in vivo experiments against bacteria, viruses, and fungi. The synergistic therapeutic interactions of BV with antibiotics has been reported. The synergistic effect contributes to a decrease in the loading and maintenance dosage, a decrease in the side effects of chemotherapy, and a decrease in drug resistance. To our knowledge, there have been no reviews on the impact of BV and its antimicrobial constituents thus far. The purpose of this review is to address the antimicrobial properties of BV and its compounds.

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The antimicrobial peptide MPX kills Actinobacillus pleuropneumoniae and reduces its pathogenicity in mice

Cited by 19 publications

References 36 publications

The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier

The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier

The Mechanism of Antimicrobial Peptide MPX against Enterohemorrhagic Escherichia coli in Vitro

Antimicrobial Properties of Apis mellifera’s Bee Venom

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